Abstract
The hypersensitive reaction (HR) is an inducible plant response that is associated with disease resistance. It is characterized by rapid, localized cell death at the site of infection and is believed to inhibit the spread of invading pathogens. We have isolated a cDNA clone, designated Hra32 (for hypersensitive reaction associated), corresponding to an RNA transcript that accumulates in bean during an HR. The predicted protein product of the Hra32 cDNA is an approximately 17 kDa protein of 161 amino acids, with four putative EF-hand calcium-binding domains. The temporal pattern of Hra32 transcript accumulation correlated closely with the onset of the HR in bean after inoculation with incompatible Pseudomonas syringae pv. tabaci and pv. tomato and with tobacco necrosis virus. Hra32 transcript also accumulated in bean in response to compatible P. syringae pv. phaseolicola and was correlated with necrotic cell death associated with disease lesion formation. A more transient pattern of Hra32 transcript accumulation occurred in bean in response to general stimuli that did not result in the HR or host cell death. These treatments included infiltration with a P. syringae pv. tabaci Hrp- mutant, P. syringae pv. tabaci cells treated with kanamycin, Escherichia coli, P. fluorescens, or glutathione, and in response to wounding. Thus, there was differential accumulation of the Hra32 transcript in response to specific stimuli resulting in the HR, compared with general stimuli that did not result in cell death. We hypothesize that the Hra32 product may be a component of the pathway that leads to hypersensitive cell death.
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Claims 7
Claim · model-written summary
A cDNA clone Hra32 was isolated corresponding to a transcript that accumulates in bean during HR.
Verbatim
e0“We have isolated a cDNA clone, designated Hra32 (for hypersensitive reaction associated), corresponding to an RNA transcript that accumulates in bean during an HR.”
Claim · model-written summary
The predicted Hra32 product is approximately 17 kDa and 161 amino acids long, with four putative EF-hand calcium-binding domains.
Verbatim
e0“The predicted protein product of the Hra32 cDNA is an approximately 17 kDa protein of 161 amino acids, with four putative EF-hand calcium-binding domains.”
t0“Bean”
Claim · model-written summary
Hra32 transcript accumulation closely correlated with HR onset after incompatible Pseudomonas syringae inoculation.
- Taxon
- beane0
- Treatment
- inoculation with incompatible Pseudomonas syringae pv. tabaci and pv. tomatoe0
- Interactor
- Pseudomonas syringae pv. tabaci and pv. tomatoPseudomonas amygdali pv. tabacie0pathogen
- Gene
- Hra32e0
- Outcome
- onset of the HRe0
Not reportedDirection
Verbatim
e0“The temporal pattern of Hra32 transcript accumulation correlated closely with the onset of the HR in bean after inoculation with incompatible Pseudomonas syringae pv. tabaci and pv. tomato”
Claim · model-written summary
Hra32 transcript accumulation closely correlated with HR onset after tobacco necrosis virus inoculation.
- Taxon
- beane0
- Treatment
- tobacco necrosis viruse0
- Interactor
- tobacco necrosis virusunidentified tobacco necrosis viruse0pathogen
- Gene
- Hra32e0
- Outcome
- onset of the HRe0
Not reportedDirection
Verbatim
e0“The temporal pattern of Hra32 transcript accumulation correlated closely with the onset of the HR in bean after inoculation with incompatible Pseudomonas syringae pv. tabaci and pv. tomato and with tobacco necrosis virus.”
Claim · model-written summary
Hra32 transcript accumulated in bean after compatible P. syringae pv. phaseolicola and correlated with lesion-associated necrotic cell death.
- Taxon
- beane0
- Treatment
- compatible P. syringae pv. phaseolicolae0
- Interactor
- P. syringae pv. phaseolicolaPseudomonas savastanoi pv. phaseolicolae0pathogen
- Gene
- Hra32e0
- Outcome
- necrotic cell death associated with disease lesion formatione0
Not reportedDirection
Verbatim
e0“Hra32 transcript also accumulated in bean in response to compatible P. syringae pv. phaseolicola and was correlated with necrotic cell death associated with disease lesion formation.”
Claim · model-written summary
General stimuli without HR or cell death produced a more transient Hra32 transcript accumulation pattern.
Verbatim
e0“A more transient pattern of Hra32 transcript accumulation occurred in bean in response to general stimuli that did not result in the HR or host cell death.”
Claim · model-written summary
The authors hypothesize Hra32 may contribute to the pathway leading to hypersensitive cell death.
Verbatim
e0“We hypothesize that the Hra32 product may be a component of the pathway that leads to hypersensitive cell death.”
t0“Bean”