Abstract
The viability of tobacco ( N. tabacum – L. cv. Xanthi‐nc) protoplasts isolated during the winter months is affected by membrane calcium, total calcium, plant age and supplementary lighting. Feeding calcium nitrate and calcium chloride helps to increase protoplast stability. Feeding is important because it increases membrane calcium, but it also has other beneficial effects which may be connected with increased nitrate and chloride (anion) uptake and cation/anion interaction. Whatever optimal feeding regime is chosen, it must be used in conjunction with the correct harvesting practice, which depends on whether or not supplementary light is given. Plants grown without supplementary light are best harvested between 8–9 weeks. If supplementary light is given, older plants (with and without supplementary calcium feeding) of approx. ten weeks are better for protoplast isolation. Plants grown under supplementary light have low leaf shape index (LSI) values (i.e. wider leaves) compared to plants in low light. Spring and summer calcium feeding treatments had no significant effect on increasing total or membrane calcium. Likewise, there was no significant correlation between total/membrane calcium and percentage protoplast viability from days 0–5; and protoplasts isolated from plants grown in shade and fed with calcium salts, were no more stable than protoplasts from control plants. Irrespective of the feed treatments given, spring and summer plants older than about fifty days and grown under shaded and normal light conditions, produced poor protoplasts despite the fact that there was more calcium present in the membranes compared to protoplasts of younger plants. This decrease in percentage protoplast viability appears to be associated with changes in leaf shape. As plants age from 49–63 days, the leaf shape index (LSI) values increase (i.e. leaves are becoming narrower) and % protoplast viability decreases.
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Claims 10
Claim · model-written summary
Feeding calcium nitrate and calcium chloride helps increase tobacco protoplast stability.
- Taxon
- tobacco
Nicotiana tabacumt0 - Treatment
- Feeding calcium nitrate and calcium chloridee1
- Outcome
- protoplast stabilitye1
- Direction
- increasee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Seasonal and physiological aspects of the isolation of tobacco protoplasts”
e1“Feeding calcium nitrate and calcium chloride helps to increase protoplast stability”
Claim · model-written summary
Tobacco plants grown without supplementary light are best harvested at 8–9 weeks.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- Plants grown without supplementary lighte2
- Outcome
- best harvestede2
- Value
- between 8–9 weekse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Seasonal and physiological aspects of the isolation of tobacco protoplasts”
e2“Plants grown without supplementary light are best harvested between 8–9 weeks”
Claim · model-written summary
Tobacco plants older, about ten weeks, are better for protoplast isolation when supplementary light is given.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- supplementary lighte3
- Outcome
- better for protoplast isolatione3
- Value
- approx. ten weekse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Seasonal and physiological aspects of the isolation of tobacco protoplasts”
e3“If supplementary light is given, older plants (with and without supplementary calcium feeding) of approx. ten weeks are better for protoplast isolation”
Claim · model-written summary
Tobacco plants grown under supplementary light have low leaf shape index values compared with plants in low light.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- supplementary lighte4
- Outcome
- leaf shape index (LSI) valuese4
- Direction
- lowe4
- Comparator
- plants in low lighte4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Seasonal and physiological aspects of the isolation of tobacco protoplasts”
e4“Plants grown under supplementary light have low leaf shape index (LSI) values (i.e. wider leaves) compared to plants in low light”
Claim · model-written summary
Spring and summer calcium feeding had no significant effect on total or membrane calcium in tobacco.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- Spring and summer calcium feeding treatmentse5
- Outcome
- total or membrane calciume5
- Direction
- no_changeinferrede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Seasonal and physiological aspects of the isolation of tobacco protoplasts”
e5“Spring and summer calcium feeding treatments had no significant effect on increasing total or membrane calcium”
Claim · model-written summary
Total or membrane calcium was not significantly correlated with percentage protoplast viability from day 0 to day 5.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- total/membrane calciume6
- Outcome
- percentage protoplast viabilitye6
- Direction
- no_changeinferrede6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Seasonal and physiological aspects of the isolation of tobacco protoplasts”
e6“there was no significant correlation between total/membrane calcium and percentage protoplast viability from days 0–5”
Claim · model-written summary
Protoplasts from shaded plants fed calcium salts were no more stable than those from control plants.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- fed with calcium saltse7
- Outcome
- stablee7
- Direction
- no_changeinferrede7
- Comparator
- control plantse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Seasonal and physiological aspects of the isolation of tobacco protoplasts”
e7“protoplasts isolated from plants grown in shade and fed with calcium salts, were no more stable than protoplasts from control plants”
Claim · model-written summary
Tobacco plants older than about fifty days produced poor protoplasts.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- plants older than about fifty dayse6
- Outcome
- poor protoplastse6
- Direction
- decreaseinferrede6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Seasonal and physiological aspects of the isolation of tobacco protoplasts”
e6“spring and summer plants older than about fifty days and grown under shaded and normal light conditions, produced poor protoplasts”
Claim · model-written summary
As tobacco plants aged from 49 to 63 days, protoplast viability decreased.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- plants age from 49–63 dayse7
- Outcome
- % protoplast viabilitye7
- Direction
- decreasese7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Seasonal and physiological aspects of the isolation of tobacco protoplasts”
e7“As plants age from 49–63 days, the leaf shape index (LSI) values increase (i.e. leaves are becoming narrower) and % protoplast viability decreases”
Claim · model-written summary
As tobacco plants aged from 49 to 63 days, leaf shape index values increased.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- plants age from 49–63 dayse7
- Outcome
- leaf shape index (LSI) valuese7
- Direction
- increasee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Seasonal and physiological aspects of the isolation of tobacco protoplasts”
e7“As plants age from 49–63 days, the leaf shape index (LSI) values increase (i.e. leaves are becoming narrower) and % protoplast viability decreases”
The same questions in other papers
calcium atom · nutrient: content · Nicotiana tabacum (tobacco)Positive and negative results
9 in the opposite direction
- positive
- positive
- positive
6 papers more in this group
See all 10 papers →infection · nutrient: content · Nicotiana tabacum (tobacco)Results agree
3 papers in the same direction as this paper
- negative
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.