Paper · 1997 · Plant Disease

Influence of Inoculation Method on Systemic Fusarium moniliforme Infection of Maize Plants Grown from Infected Seeds

G. P. Munkvold; W. M. Carlton

7
claims
14
verbatim quotes
89
citations
1
research areas
—
Not open access

Abstract

Two greenhouse and two field experiments were conducted in 1994 and 1995 to quantify the incidence of maize kernel infection resulting from systemic infection of maize plants by Fusarium moniliforme. Seeds were infected by two methods: (i) spray-inoculation of maize silks during seed development (field-infected), and (ii) soak-inoculation of mature seeds in a spore suspension (laboratory-infected). Plants were grown from infected seeds and assayed for systemic infection by the seed-inoculated strain, determined by vegetative compatibility of recovered isolates with the original strain. The seed-inoculated strain was recovered from stalks and kernels of plants grown from both types of infected seed. Mature plants grown from field-infected seeds had a higher percentage of their kernels infected with the seed-inoculated strain compared with plants from laboratory-infected seed. Mean incidence of infection by the seed-inoculated strain was 9.9 to 29.4% of all kernels (33.0 to 55.9% of F. moniliforme-infected kernels) in the plants grown from field-infected seed. Some plants from infected seed were subsequently silk-inoculated, and the silk-inoculated strain was recovered from a much higher percentage of kernels (26.5 to 37.5% of all kernels or 77.9 to 78.4% of F. moniliforme-infected kernels) than was the seed-inoculated strain; furthermore, silk inoculation significantly reduced incidence of kernel infection by the seed-inoculated strain. Seed infection by F. moniliforme resulted in systemic infection of plants and kernels. However, local infection (via silks) was a more important pathway to kernels than was systemic infection, and strains infecting the silks competed successfully against those entering the kernels through systemic development.

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Claims 7

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Plants grown from field-infected seed had a higher percentage of kernels infected by the seed-inoculated strain than plants from laboratory-infected seed.

Taxon
maizeZea mayse0
Organ / stage
kernelse1
Treatment
field-infected seedse1
Interactor
Fusarium moniliformeFusarium verticillioidese0pathogen
Outcome
percentage of their kernels infected with the seed-inoculated straine1
Direction
highere1
Comparator
laboratory-infected seede1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗
Fusarium verticillioides
Fusarium verticillioides · InteractorPublic domain · Wikimedia Commons ↗

Verbatim

e0

“Two greenhouse and two field experiments were conducted in 1994 and 1995 to quantify the incidence of maize kernel infection resulting from systemic infection of maize plants by Fusarium moniliforme”

✓ located verbatim in the source · abstract, characters 0–198 · show in text ↑
e1

“Mature plants grown from field-infected seeds had a higher percentage of their kernels infected with the seed-inoculated strain compared with plants from laboratory-infected seed”

✓ located verbatim in the source · abstract, characters 708–886 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Incidence of infection by the seed-inoculated strain ranged from 9.9 to 29.4% of all kernels in plants grown from field-infected seed.

Taxon
maizeZea mayse0
Organ / stage
kernelse2
Interactor
F. moniliformeFusarium verticillioidese2pathogen
Outcome
incidence of infection by the seed-inoculated straine2
Value
9.9 to 29.4%e2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗
Fusarium verticillioides
Fusarium verticillioides · InteractorPublic domain · Wikimedia Commons ↗

Verbatim

e0

“Two greenhouse and two field experiments were conducted in 1994 and 1995 to quantify the incidence of maize kernel infection resulting from systemic infection of maize plants by Fusarium moniliforme”

✓ located verbatim in the source · abstract, characters 0–198 · show in text ↑
e2

“Mean incidence of infection by the seed-inoculated strain was 9.9 to 29.4% of all kernels (33.0 to 55.9% of F. moniliforme-infected kernels) in the plants grown from field-infected seed”

✓ located verbatim in the source · abstract, characters 888–1073 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Silk-inoculated strain was recovered from a much higher percentage of kernels than the seed-inoculated strain, at 26.5 to 37.5% of all kernels.

Taxon
maizeZea mayse0
Treatment
silk-inoculatede3
Interactor
F. moniliformeFusarium verticillioidese3pathogen
Outcome
percentage of kernelse3
Direction
highere3
Value
26.5 to 37.5%e3
Comparator
seed-inoculated straine3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗
Fusarium verticillioides
Fusarium verticillioides · InteractorPublic domain · Wikimedia Commons ↗

Verbatim

e0

“Two greenhouse and two field experiments were conducted in 1994 and 1995 to quantify the incidence of maize kernel infection resulting from systemic infection of maize plants by Fusarium moniliforme”

✓ located verbatim in the source · abstract, characters 0–198 · show in text ↑
e3

“Some plants from infected seed were subsequently silk-inoculated, and the silk-inoculated strain was recovered from a much higher percentage of kernels (26.5 to 37.5% of all kernels or 77.9 to 78.4% of F. moniliforme-infected kernels) than was the seed-inoculated strain”

✓ located verbatim in the source · abstract, characters 1075–1345 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Silk inoculation significantly reduced kernel infection by the seed-inoculated strain.

Taxon
maizeZea mayse0
Treatment
silk inoculatione4
Interactor
Fusarium moniliformeFusarium verticillioidese0pathogen
Outcome
incidence of kernel infection by the seed-inoculated straine4
Direction
reducede4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗
Fusarium verticillioides
Fusarium verticillioides · InteractorPublic domain · Wikimedia Commons ↗

Verbatim

e0

“Two greenhouse and two field experiments were conducted in 1994 and 1995 to quantify the incidence of maize kernel infection resulting from systemic infection of maize plants by Fusarium moniliforme”

✓ located verbatim in the source · abstract, characters 0–198 · show in text ↑
e4

“furthermore, silk inoculation significantly reduced incidence of kernel infection by the seed-inoculated strain”

✓ located verbatim in the source · abstract, characters 1347–1458 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Seed infection by Fusarium moniliforme resulted in systemic infection of plants and kernels.

Taxon
maizeZea mayse0
Treatment
Seed infectione5
Interactor
F. moniliformeFusarium verticillioidese5pathogen
Outcome
systemic infection of plants and kernelse5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗
Fusarium verticillioides
Fusarium verticillioides · InteractorPublic domain · Wikimedia Commons ↗

Verbatim

e0

“Two greenhouse and two field experiments were conducted in 1994 and 1995 to quantify the incidence of maize kernel infection resulting from systemic infection of maize plants by Fusarium moniliforme”

✓ located verbatim in the source · abstract, characters 0–198 · show in text ↑
e5

“Seed infection by F. moniliforme resulted in systemic infection of plants and kernels”

✓ located verbatim in the source · abstract, characters 1460–1545 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Local infection via silks was a more important pathway to kernels than systemic infection.

Taxon
maizeZea mayse0
Treatment
local infection (via silks)e6
Interactor
Fusarium moniliformeFusarium verticillioidese0pathogen
Outcome
pathway to kernelse6
Direction
more importante6
Comparator
systemic infectione6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗
Fusarium verticillioides
Fusarium verticillioides · InteractorPublic domain · Wikimedia Commons ↗

Verbatim

e0

“Two greenhouse and two field experiments were conducted in 1994 and 1995 to quantify the incidence of maize kernel infection resulting from systemic infection of maize plants by Fusarium moniliforme”

✓ located verbatim in the source · abstract, characters 0–198 · show in text ↑
e6

“However, local infection (via silks) was a more important pathway to kernels than was systemic infection”

✓ located verbatim in the source · abstract, characters 1547–1651 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Strains infecting the silks competed successfully against those entering the kernels through systemic development.

Taxon
maizeZea mayse0
Interactor
Fusarium moniliformeFusarium verticillioidese0pathogen
Outcome
competed successfullye7
Qualifier
against those entering the kernels through systemic developmente7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗
Fusarium verticillioides
Fusarium verticillioides · InteractorPublic domain · Wikimedia Commons ↗

Verbatim

e0

“Two greenhouse and two field experiments were conducted in 1994 and 1995 to quantify the incidence of maize kernel infection resulting from systemic infection of maize plants by Fusarium moniliforme”

✓ located verbatim in the source · abstract, characters 0–198 · show in text ↑
e7

“strains infecting the silks competed successfully against those entering the kernels through systemic development”

✓ located verbatim in the source · abstract, characters 1657–1770 · show in text ↑

inoculation · Fusarium verticillioides · Zea mays (maize)Positive and negative results

25 papers: 14 positive, 11 negative or no effect

13 papers in the same direction as this paper · 11 in the opposite direction

  1. negative
  2. negative
  3. negative

21 papers more in this group

See all 25 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.