Abstract
Callus cultures ofArachis hypogaea L. cv. JL-24 adapted to 200 mM NaCl (otherwise lethal to cells) were used for the study. Calli grew slowly when transferred to 250 mM NaCl, but the growth was enhanced when ABA was included in the medium. ABA induced increase in growth of callus was not accompanied by corresponding increase in internal free proline levels. 0.5 mM of CaCl2 ameliorated the negative effect of NaCl indicating that cells require a specific Ca2+/Na+ ratio for their growth. Proline content also increased at this ratio thereby suggesting that increase in growth at 0.5 mM Ca2+ may be due to an increase in proline content. However, exogenous proline did not increase the growth of callus (adapted to 200 mM), and higher concentrations even inhibited the growth. This shows that proline is not required for growth or adaptation of cells to salt stress, but is produced as a consequence of stress.
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Claims 10
Claim · model-written summary
Calli grew slowly in 250 mM NaCl.
- Taxon
- Arachis hypogaea L.
Arachis hypogaeae1 - Treatment
- 250 mM NaCle2
- Outcome
- growthe2
- Direction
- grew slowlye2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Callus cultures ofArachis hypogaea L. cv. JL-24 adapted to 200 mM NaCl (otherwise lethal to cells) were used for the study”
e2“Calli grew slowly when transferred to 250 mM NaCl, but the growth was enhanced when ABA was included in the medium”
Claim · model-written summary
ABA enhanced callus growth at 250 mM NaCl.
- Taxon
- Arachis hypogaea L.Arachis hypogaeae1
- Treatment
- ABAe2
- Outcome
- growthe2
- Direction
- enhancede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Callus cultures ofArachis hypogaea L. cv. JL-24 adapted to 200 mM NaCl (otherwise lethal to cells) were used for the study”
e2“Calli grew slowly when transferred to 250 mM NaCl, but the growth was enhanced when ABA was included in the medium”
Claim · model-written summary
0.5 mM CaCl2 ameliorated the negative effect of NaCl on callus growth.
- Taxon
- Arachis hypogaea L.Arachis hypogaeae1
- Treatment
- 0.5 mM of CaCl2e3
- Outcome
- the negative effect of NaCle3
- Direction
- amelioratede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Callus cultures ofArachis hypogaea L. cv. JL-24 adapted to 200 mM NaCl (otherwise lethal to cells) were used for the study”
e3“0.5 mM of CaCl2 ameliorated the negative effect of NaCl indicating that cells require a specific Ca2+/Na+ ratio for their growth”
Claim · model-written summary
The authors suggest that cells require a specific Ca2+/Na+ ratio for growth.
- Taxon
- Arachis hypogaea L.Arachis hypogaeae1
- Compound
- Ca2+/Na+ ratioe3
- Outcome
- growthe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Callus cultures ofArachis hypogaea L. cv. JL-24 adapted to 200 mM NaCl (otherwise lethal to cells) were used for the study”
e3“0.5 mM of CaCl2 ameliorated the negative effect of NaCl indicating that cells require a specific Ca2+/Na+ ratio for their growth”
Claim · model-written summary
Proline content increased at the 0.5 mM CaCl2 ratio.
- Taxon
- Arachis hypogaea L.Arachis hypogaeae1
- Treatment
- 0.5 mM of CaCl2e3
- Outcome
- Proline contente4
- Direction
- increasede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Callus cultures ofArachis hypogaea L. cv. JL-24 adapted to 200 mM NaCl (otherwise lethal to cells) were used for the study”
e3“0.5 mM of CaCl2 ameliorated the negative effect of NaCl indicating that cells require a specific Ca2+/Na+ ratio for their growth”
e4“Proline content also increased at this ratio”
Claim · model-written summary
The growth increase at 0.5 mM Ca2+ may be due to increased proline content.
- Taxon
- Arachis hypogaea L.Arachis hypogaeae1
- Compound
- prolinee5
- Outcome
- increase in growth at 0.5 mM Ca2+e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Callus cultures ofArachis hypogaea L. cv. JL-24 adapted to 200 mM NaCl (otherwise lethal to cells) were used for the study”
e5“increase in growth at 0.5 mM Ca2+ may be due to an increase in proline content”
Claim · model-written summary
Exogenous proline did not increase callus growth at 200 mM NaCl.
- Taxon
- Arachis hypogaea L.Arachis hypogaeae1
- Treatment
- exogenous prolinee6
- Outcome
- growth of calluse6
- Direction
- did not increasee6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Callus cultures ofArachis hypogaea L. cv. JL-24 adapted to 200 mM NaCl (otherwise lethal to cells) were used for the study”
e6“exogenous proline did not increase the growth of callus (adapted to 200 mM), and higher concentrations even inhibited the growth”
Claim · model-written summary
Higher concentrations of exogenous proline inhibited callus growth.
- Taxon
- Arachis hypogaea L.Arachis hypogaeae1
- Treatment
- higher concentrationse6
- Outcome
- growthe6
- Direction
- inhibitede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Callus cultures ofArachis hypogaea L. cv. JL-24 adapted to 200 mM NaCl (otherwise lethal to cells) were used for the study”
e6“exogenous proline did not increase the growth of callus (adapted to 200 mM), and higher concentrations even inhibited the growth”
Claim · model-written summary
Proline is not required for growth or adaptation of callus cells to salt stress.
- Taxon
- Arachis hypogaea L.Arachis hypogaeae1
- Compound
- prolinee7
- Outcome
- growth or adaptation of cells to salt stresse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Callus cultures ofArachis hypogaea L. cv. JL-24 adapted to 200 mM NaCl (otherwise lethal to cells) were used for the study”
e7“proline is not required for growth or adaptation of cells to salt stress, but is produced as a consequence of stress”
Claim · model-written summary
Proline is produced as a consequence of salt stress in callus.
- Taxon
- Arachis hypogaea L.Arachis hypogaeae1
- Compound
- prolinee7
- Outcome
- produced as a consequence of stresse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Callus cultures ofArachis hypogaea L. cv. JL-24 adapted to 200 mM NaCl (otherwise lethal to cells) were used for the study”
e7“proline is not required for growth or adaptation of cells to salt stress, but is produced as a consequence of stress”
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1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.