Abstract
Infection by Spongospora subterranea of roots of two potato ( Solanum tuberosum ) cultivars, either very resistant or very susceptible to powdery scab on their tubers, was studied in a glasshouse experiment. Plants grown in sand/nutrient solution culture were inoculated with S. subterranea sporosori 2 weeks after planting. Plant parameters, the intensity of zoosporangium infection in roots, numbers of Spongospora root galls and amounts of Spongospora DNA in roots, measured using quantitative PCR ( qPCR ), were assessed at sequential harvests. Inoculation with S. subterranea reduced water use (56 days after planting) by 26% in the tuber resistant cultivar compared with uninoculated plants, and by 60% in the susceptible cultivar. Inoculation did not affect growth of the resistant cultivar, nor shoot mass of the susceptible cultivar, but caused a 38% reduction in root mass of the susceptible cultivar. The intensities of zoosporangium development in both cultivars were similar. The susceptible cultivar had approximately four times more Spongospora root galls g −1 root mass than the resistant cultivar. Quantitative PCR detected S. subterranea DNA in roots 1 week after inoculation, and indicated a twofold greater amount of pathogen DNA in roots of the susceptible than the resistant cultivar. This study suggests that the S. subterranea zoosporangium stage in host roots is affected differently by host resistance factors than the sporosorus (root gall and tuber scab) stages. The study has also demonstrated the usefulness of qPCR for sensitive and consistent detection of S. subterranea across the duration of potato root infection.
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Claims 11
Claim · model-written summary
Inoculation reduced water use by 26% in the tuber resistant cultivar.
- Taxon
- potato
Solanum tuberosumt0 - Material
- tuber resistant cultivare0
- Treatment
- Inoculation with S. subterraneae0
- Interactor
- S. subterranea
Spongospora subterraneae0pathogen - Outcome
- water usee0
- Direction
- reducede0
- Value
- 26%e0
- Comparator
- uninoculated plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inoculation with S. subterranea reduced water use (56 days after planting) by 26% in the tuber resistant cultivar compared with uninoculated plants”
t0“potato”
Claim · model-written summary
Inoculation reduced water use by 60% in the susceptible cultivar.
- Taxon
- potatoSolanum tuberosumt0
- Material
- susceptible cultivare0
- Treatment
- Inoculation with S. subterraneae0
- Interactor
- S. subterraneaSpongospora subterraneae0pathogen
- Outcome
- water usee0
- Direction
- reducede0
- Value
- 60%e0
- Comparator
- uninoculated plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inoculation with S. subterranea reduced water use (56 days after planting) by 26% in the tuber resistant cultivar compared with uninoculated plants, and by 60% in the susceptible cultivar”
t0“potato”
Claim · model-written summary
Inoculation did not affect growth of the resistant cultivar.
- Taxon
- potatoSolanum tuberosumt0
- Material
- resistant cultivare0
- Treatment
- Inoculatione0
- Outcome
- growthe0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inoculation did not affect growth of the resistant cultivar”
t0“potato”
Claim · model-written summary
Inoculation did not affect shoot mass of the susceptible cultivar.
- Taxon
- potatoSolanum tuberosumt0
- Material
- susceptible cultivare0
- Treatment
- Inoculatione0
- Outcome
- shoot masse0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inoculation did not affect growth of the resistant cultivar, nor shoot mass of the susceptible cultivar”
t0“potato”
Claim · model-written summary
Inoculation reduced root mass of the susceptible cultivar by 38%.
- Taxon
- potatoSolanum tuberosumt0
- Material
- susceptible cultivare0
- Treatment
- Inoculatione0
- Outcome
- root masse0
- Direction
- reductione0
- Value
- 38%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inoculation did not affect growth of the resistant cultivar, nor shoot mass of the susceptible cultivar, but caused a 38% reduction in root mass of the susceptible cultivar”
t0“potato”
Claim · model-written summary
Zoosporangium development intensity was similar in both cultivars.
- Taxon
- potatoSolanum tuberosumt0
- Interactor
- zoosporangiume0pathogen
- Outcome
- intensities of zoosporangium developmente0
- Direction
- similare0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The intensities of zoosporangium development in both cultivars were similar”
t0“potato”
Claim · model-written summary
The susceptible cultivar had about four times as many root galls per root mass as the resistant cultivar.
- Taxon
- potatoSolanum tuberosumt0
- Material
- susceptible cultivare0
- Interactor
- SpongosporaSpongosporae0pathogen
- Outcome
- Spongospora root galls g −1 root masse0
- Direction
- moree0
- Value
- approximately four timese0
- Comparator
- resistant cultivare0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The susceptible cultivar had approximately four times more Spongospora root galls g −1 root mass than the resistant cultivar”
t0“potato”
Claim · model-written summary
qPCR detected pathogen DNA in roots one week after inoculation.
- Taxon
- potatoSolanum tuberosumt0
- Interactor
- S. subterraneaSpongospora subterraneae0pathogen
- Outcome
- S. subterranea DNA in rootse0
- Value
- 1 weeke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Quantitative PCR detected S. subterranea DNA in roots 1 week after inoculation”
t0“potato”
Claim · model-written summary
Susceptible cultivar roots contained twice as much pathogen DNA as resistant cultivar roots.
- Taxon
- potatoSolanum tuberosumt0
- Material
- susceptiblee0
- Outcome
- pathogen DNA in rootse0
- Direction
- greatere0
- Value
- twofolde0
- Comparator
- resistant cultivare0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a twofold greater amount of pathogen DNA in roots of the susceptible than the resistant cultivar”
t0“potato”
Claim · model-written summary
The zoosporangium stage appears to respond differently to host resistance factors than sporosorus stages.
- Taxon
- potatoSolanum tuberosumt0
- Interactor
- S. subterraneaSpongospora subterraneae0pathogen
- Outcome
- zoosporangium stage in host rootse0
- Qualifier
- host resistance factorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the S. subterranea zoosporangium stage in host roots is affected differently by host resistance factors than the sporosorus (root gall and tuber scab) stages”
t0“potato”
Claim · model-written summary
qPCR was useful for sensitive and consistent detection through potato root infection.
- Taxon
- potatoSolanum tuberosume0
- Interactor
- S. subterraneaSpongospora subterraneae0pathogen
- Outcome
- sensitive and consistent detection of S. subterraneae0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“usefulness of qPCR for sensitive and consistent detection of S. subterranea across the duration of potato root infection”
t0“potato”
The same questions in other papers
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1 paper in the same direction as this paper
- negative
inoculation · physiology: water · Solanum tuberosum (potato)Positive and negative results
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21 papers in the same direction as this paper · 82 in the opposite direction
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100 papers more in this group
See all 104 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.