Paper · 2005

Practical use of PCR-based method for detection of tumorigenic Agrobacterium in soil.

P. Sobiczewski; Joanna Puławska; S. Berczyński

5
claims
8
verbatim quotes
4
citations
2
research areas
—
Not open access

Abstract

The presence of tumorigenic Agrobacterium was studied in soil of three separate fields used for cultivation of cereals, grass and fruit nursery, respectively. Bacteria were detected with semi-nested PCR-based method and sunflower biotest. They were found in 54–71% of samples originating from stone fruit trees nursery (with crown gall problem), whereas only in 1.4–8.3% of those collected from meadow. The higher percentage of contaminated samples was found when analyses were performed later in spring. Of 168 rye field soil samples only one contained pathogenic Agrobacterium. None of the sunflower seedlings inoculated with bacteria, which were grown on agar medium as a result of introduction water suspension of tested soil samples, showed crown gall symptoms after 30 days from inoculation.

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Claims 5

Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Tumorigenic Agrobacterium was detected in 54–71% of stone fruit nursery soil samples.

Taxon
stone fruit treese0
Interactor
AgrobacteriumAgrobacteriumt0pathogen
Outcome
They were founde0
Value
54–71%e0
Setting
stone fruit trees nurserye0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Agrobacterium
Agrobacterium · Interactor · picture from its Wikimedia Commons categoryDidierFy · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“They were found in 54–71% of samples originating from stone fruit trees nursery (with crown gall problem)”

✓ located verbatim in the source · abstract, characters 239–344 · show in text ↑
t0

“Agrobacterium”

✓ located verbatim in the source · title, characters 63–76 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Tumorigenic Agrobacterium was detected in 1.4–8.3% of meadow soil samples.

Interactor
AgrobacteriumAgrobacteriumt0pathogen
Outcome
those collected from meadowe0
Value
1.4–8.3%e0
Setting
meadowe0

Not reportedTaxon

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Agrobacterium
Agrobacterium · Interactor · picture from its Wikimedia Commons categoryDidierFy · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“whereas only in 1.4–8.3% of those collected from meadow”

✓ located verbatim in the source · abstract, characters 346–401 · show in text ↑
t0

“Agrobacterium in soil”

✓ located verbatim in the source · title, characters 63–84 · show in text ↑
Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

The fraction of contaminated samples was higher later in spring.

Interactor
AgrobacteriumAgrobacteriumt0pathogen
Outcome
percentage of contaminated samplese0
Direction
highere0
Setting
later in springe0

Not reportedTaxon

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Agrobacterium
Agrobacterium · Interactor · picture from its Wikimedia Commons categoryDidierFy · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The higher percentage of contaminated samples was found when analyses were performed later in spring.”

✓ located verbatim in the source · abstract, characters 403–504 · show in text ↑
t0

“Agrobacterium in soil.”

✓ located verbatim in the source · title, characters 63–85 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

One of 168 rye field soil samples contained pathogenic Agrobacterium.

Interactor
AgrobacteriumAgrobacteriume0pathogen
Outcome
contained pathogenic Agrobacteriume0
Value
onee0
Setting
rye fielde0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Secale cereale
Secale cereale · TaxonLSDSL · CC BY-SA 3.0 · Wikimedia Commons ↗
Agrobacterium
Agrobacterium · Interactor · picture from its Wikimedia Commons categoryDidierFy · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Of 168 rye field soil samples only one contained pathogenic Agrobacterium.”

✓ located verbatim in the source · abstract, characters 505–579 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

No inoculated sunflower seedlings showed crown gall symptoms after 30 days.

Taxon
sunflowerHelianthus annuuse0
Treatment
inoculated with bacteriae0
Interactor
bacteriaBacteriae0pathogen
Outcome
crown gall symptomse0
Direction
Nonee0
Qualifier
after 30 days from inoculatione0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Helianthus annuus
Helianthus annuus · TaxonJon Sullivan · Public domain · Wikimedia Commons ↗

Verbatim

e0

“None of the sunflower seedlings inoculated with bacteria, which were grown on agar medium as a result of introduction water suspension of tested soil samples, showed crown gall symptoms after 30 days from inoculation.”

✓ located verbatim in the source · abstract, characters 580–797 · show in text ↑

inoculation · Bacteria · Helianthus annuus (common sunflower)Positive and negative results

6 papers: 5 positive, 1 negative or no effect

5 in the opposite direction

  1. positive
  2. positive
  3. positive

2 papers more in this group

See all 6 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.