Abstract
ABSTRACT Banana polyphenoloxidases oxidize specifically o ‐diphenols. The Km values were low for dopamine, epinephrine, and norepinephrine. It was lower for L‐dopa as compared with D‐dopa. The most effective inhibitor was ascorbic acid followed by cysteine and then sodium metabisulfite. Diethyldithiocarbamate, at pH 7.0, was not as effective as the above inhibitors. Ammonium ion and the oxidized β‐nicotine adenine dinucleotide acted as activators whereas Fe +2 , Fe+ 3 , Al +3 , Ca +2 and Zn +2 showed various degrees of inhibition. On the other hand, C +1 , Cu +2 , Mg +2 and Mn +2 did not affect the enzyme activity. Mercaptoethanol (17 mM) completely inactivated the enzymes. On dialysis 30% of the activity was restored with regeneration of two isozymes.
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Claims 19
Claim · model-written summary
Banana polyphenoloxidases oxidize o-diphenols specifically.
Not reportedDirection
Verbatim
t0“Banana”
e0“Banana polyphenoloxidases oxidize specifically o ‐diphenols”
Claim · model-written summary
Km values of banana polyphenoloxidase were low for dopamine, epinephrine and norepinephrine.
Verbatim
t0“Banana”
e0“The Km values were low for dopamine, epinephrine, and norepinephrine”
Claim · model-written summary
The Km of banana polyphenoloxidase was lower for L-dopa than for D-dopa.
Verbatim
t0“Banana”
e0“It was lower for L‐dopa as compared with D‐dopa”
Claim · model-written summary
The most effective inhibitor of banana polyphenoloxidase was ascorbic acid, followed by cysteine and then sodium metabisulfite.
Not reportedDirection
Verbatim
t0“Banana”
e0“The most effective inhibitor was ascorbic acid followed by cysteine and then sodium metabisulfite”
Claim · model-written summary
Diethyldithiocarbamate at pH 7.0 was not as effective an inhibitor of banana polyphenoloxidase as ascorbic acid, cysteine and sodium metabisulfite.
Verbatim
t0“Banana”
e0“Diethyldithiocarbamate, at pH 7.0, was not as effective as the above inhibitors”
Claim · model-written summary
Ammonium ion acted as an activator of banana polyphenoloxidase.
Not reportedComparator
Verbatim
t0“Banana”
e0“Ammonium ion and the oxidized β‐nicotine adenine dinucleotide acted as activators”
Claim · model-written summary
Oxidized β‐nicotine adenine dinucleotide acted as an activator of banana polyphenoloxidase.
- Taxon
- Bananat0
- Treatment
- oxidized β‐nicotine adenine dinucleotidee0
- Outcome
- acted as activatorse0
- Direction
- activatorse0
Not reportedComparator
Verbatim
t0“Banana”
e0“Ammonium ion and the oxidized β‐nicotine adenine dinucleotide acted as activators”
Claim · model-written summary
Fe +2 showed a degree of inhibition of banana polyphenoloxidase activity.
Not reportedComparator
Verbatim
t0“Banana”
e0“Fe +2 , Fe+ 3 , Al +3 , Ca +2 and Zn +2 showed various degrees of inhibition”
Claim · model-written summary
Fe+ 3 showed a degree of inhibition of banana polyphenoloxidase activity.
Not reportedComparator
Verbatim
t0“Banana”
e0“Fe +2 , Fe+ 3 , Al +3 , Ca +2 and Zn +2 showed various degrees of inhibition”
Claim · model-written summary
Al +3 showed a degree of inhibition of banana polyphenoloxidase activity.
Not reportedComparator
Verbatim
t0“Banana”
e0“Fe +2 , Fe+ 3 , Al +3 , Ca +2 and Zn +2 showed various degrees of inhibition”
Claim · model-written summary
Ca +2 showed a degree of inhibition of banana polyphenoloxidase activity.
Not reportedComparator
Verbatim
t0“Banana”
e0“Fe +2 , Fe+ 3 , Al +3 , Ca +2 and Zn +2 showed various degrees of inhibition”
Claim · model-written summary
Zn +2 showed a degree of inhibition of banana polyphenoloxidase activity.
Not reportedComparator
Verbatim
t0“Banana”
e0“Fe +2 , Fe+ 3 , Al +3 , Ca +2 and Zn +2 showed various degrees of inhibition”
Claim · model-written summary
C +1 did not affect banana polyphenoloxidase activity.
Not reportedComparator
Verbatim
t0“Banana”
e0“C +1 , Cu +2 , Mg +2 and Mn +2 did not affect the enzyme activity”
Claim · model-written summary
Cu +2 did not affect banana polyphenoloxidase activity.
Not reportedComparator
Verbatim
t0“Banana”
e0“C +1 , Cu +2 , Mg +2 and Mn +2 did not affect the enzyme activity”
Claim · model-written summary
Mg +2 did not affect banana polyphenoloxidase activity.
Not reportedComparator
Verbatim
t0“Banana”
e0“C +1 , Cu +2 , Mg +2 and Mn +2 did not affect the enzyme activity”
Claim · model-written summary
Mn +2 did not affect banana polyphenoloxidase activity.
Not reportedComparator
Verbatim
t0“Banana”
e0“C +1 , Cu +2 , Mg +2 and Mn +2 did not affect the enzyme activity”
Claim · model-written summary
Mercaptoethanol at 17 mM completely inactivated the banana polyphenoloxidases.
- Taxon
- Bananat0
- Treatment
- Mercaptoethanol (17 mM)e0
- Outcome
- the enzymese0
- Direction
- completely inactivatede0
Not reportedComparator
Verbatim
t0“Banana”
e0“Mercaptoethanol (17 mM) completely inactivated the enzymes”
Claim · model-written summary
On dialysis, 30% of the polyphenoloxidase activity was restored.
Not reportedComparator
Verbatim
t0“Banana”
e0“On dialysis 30% of the activity was restored”
Claim · model-written summary
Dialysis was accompanied by regeneration of two polyphenoloxidase isozymes.
Not reportedDirection
Verbatim
t0“Banana”
e0“On dialysis 30% of the activity was restored with regeneration of two isozymes”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.