Abstract
Embryos were excised from the seeds of naked barley after 24 hours soaking and were irradiated with 1, 2 and 3 kR of 137Cs γ-rays. Microsomal fractions of these embryos were separated by sucrose gradient centrifugation. Cells of germinating seed embryo have contained both free and bound ribosomes. The ratio of bound to free ribosomes decreased with an increase in dose. There was no significant difference in the yield of microsomal fraction between the nonirradiated and the irradiated except 3 kR. Thus, it was concluded that the yield of free ribosomes increased at the expense of the bound ribosomes by 1 and 2 kR irradiation, and that at 3 kR the degradation of ribosomes per se, in addition to detachment of bound ribosomes from the membranes, occurred. For only irradiated samples, membrane-like substance was found visibly floating in ethanol solution during the process of extracting RNA from the 105, 000 × g supernatant.オオムギ種子を24時間浸漬したのち, 1, 2, 3kRの137Csγ線を照射し, ただちにミクロゾーム画分を分離した。オオムギ種子胚の細胞には遊離型と膜付着型との2種類のリボゾームが存在し, 膜付着型/遊離型の量比は照射線量の増加とともに減少した。ミクロゾーム量の回収率は3kR照射の場合を除いて照射と無照射との間に有意な差異が認められなかった。膜付着型/遊離型の量比の減少は膜付着型リボゾームが照射によって遊離型リボゾームに変化したためと思われる。3kR照射ではこのような変化のほかにリボゾームそのものも分解することが示唆された。照射試料由来の105, 000×g上澄み画分からRNAを抽出する過程でエタノール中に膜様物質の浮遊が可視的に認められた。
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
Germinating barley embryo cells contained free and bound ribosomes.
- Taxon
- Barley
.jpg?width=60)
Hordeum vulgaret0 - Organ / stage
- germinating seed embryoe0
- Outcome
- both free and bound ribosomese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Cells of germinating seed embryo have contained both free and bound ribosomes.”
t0“Barley Seeds”
Claim · model-written summary
The bound-to-free ribosome ratio decreased as irradiation dose increased.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- dosee0
- Outcome
- ratio of bound to free ribosomese0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ratio of bound to free ribosomes decreased with an increase in dose.”
t0“Barley Seeds”
Claim · model-written summary
Microsomal fraction yield did not differ significantly between nonirradiated and irradiated embryos except at 3 kR.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- irradiatede0
- Outcome
- yield of microsomal fractione0
- Direction
- no significant differencee0
- Qualifier
- except 3 kRe0
- Comparator
- nonirradiatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There was no significant difference in the yield of microsomal fraction between the nonirradiated and the irradiated except 3 kR.”
t0“Barley Seeds”
Claim · model-written summary
The authors concluded that 1 and 2 kR irradiation increased free ribosomes at the expense of bound ribosomes.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- 1 and 2 kR irradiatione0
- Outcome
- yield of free ribosomese0
- Direction
- increasede0
- Qualifier
- at the expense of the bound ribosomese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the yield of free ribosomes increased at the expense of the bound ribosomes by 1 and 2 kR irradiation”
t0“Barley Seeds”
Claim · model-written summary
At 3 kR, the authors inferred ribosome degradation alongside detachment from membranes.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- 3 kRe0
- Outcome
- degradation of ribosomes per see0
- Qualifier
- detachment of bound ribosomes from the membranese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“at 3 kR the degradation of ribosomes per se, in addition to detachment of bound ribosomes from the membranes, occurred”
t0“Barley Seeds”
Claim · model-written summary
Membrane-like substance was visible in ethanol during RNA extraction only for irradiated samples.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- irradiated samplese0
- Outcome
- membrane-like substance was found visibly floating in ethanol solutione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“For only irradiated samples, membrane-like substance was found visibly floating in ethanol solution during the process of extracting RNA from the 105, 000 × g supernatant.”
t0“Barley Seeds”
The same questions in other papers
radiation · yield · Hordeum vulgare (barley)Positive and negative results
2 papers in the same direction as this paper · 9 in the opposite direction
- positive
- positive
- positive
8 papers more in this group
See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.