Abstract
In the present experiment, we studied the interaction between copper (Cu) and iron (Fe) in strawberry plants grown in nutrient solutions containing different concentrations of Fe. Plants grown in the absence of iron (Fe0) had the characteristic symptoms of Fe deficiency, with smaller chlorotic leaves, less biomass, acidification of the nutrient solution, and roots that were smaller and less ramified, while no symptoms of Fe deficiency were observed in plants grown with Fe. A greater amount of Cu was found in roots of chlorotic plants than in those grown with Fe, while plants grown with 20 μM of Fe (Fe20) in the nutrient solution had a greater amount of Fe compared with plants from the other treatments. Chlorotic plants (Fe0) and plants grown with the greatest level of Fe (Fe20) had a greater root ferric chelate reductase (FC-R; EC 1.16.1.17) activity compared with the other treatments with 5 or 10 μM Fe in the nutrient solution. The same pattern was obtained for relative FC-R mRNA concentration and for the sum of Fe and Cu contents in shoots (leaves plus crowns). The DNA obtained from amplification of the FC-R mRNA was cloned and several of the inserts analysed by single strand confirmation polymorphism (SSCP). Although there were different SSCP patterns in the Fe20 treatment, all the inserts that were sequenced were very similar, excluding the hypothesis of more than one FC-R mRNA species being present. The results suggest that Cu as well as Fe is involved in FC-R expression and activity, although the mechanism involved in this regulation is unknown so far. Both small contents of Fe and Cu in plants led to an over-expression of the FC-R gene and enhanced FC-R activity in strawberry roots.
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Claims 9
Claim · model-written summary
Iron deprivation produced chlorotic leaves and reduced biomass and root development.
Verbatim
e0“Plants grown in the absence of iron (Fe0) had the characteristic symptoms of Fe deficiency, with smaller chlorotic leaves, less biomass, acidification of the nutrient solution, and roots that were smaller and less ramified, while no symptoms of Fe deficiency were observed in plants grown with Fe”
Claim · model-written summary
Iron deprivation acidified the nutrient solution.
Verbatim
e0“Plants grown in the absence of iron (Fe0) had the characteristic symptoms of Fe deficiency, with smaller chlorotic leaves, less biomass, acidification of the nutrient solution, and roots that were smaller and less ramified, while no symptoms of Fe deficiency were observed in plants grown with Fe”
Claim · model-written summary
Chlorotic roots contained more copper than roots grown with iron.
- Taxon
- plantsViridiplantaee0
- Organ / stage
- rootse0
- Outcome
- amount of Cue0
- Direction
- greatere0
Verbatim
e0“A greater amount of Cu was found in roots of chlorotic plants than in those grown with Fe”
Claim · model-written summary
Plants given 20 μM iron contained more iron than other treatments.
Verbatim
e0“plants grown with 20 μM of Fe (Fe20) in the nutrient solution had a greater amount of Fe compared with plants from the other treatments”
Claim · model-written summary
Fe0 and Fe20 plants had greater root ferric chelate reductase activity than Fe5 or Fe10 plants.
Verbatim
e0“Chlorotic plants (Fe0) and plants grown with the greatest level of Fe (Fe20) had a greater root ferric chelate reductase (FC-R; EC 1.16.1.17) activity compared with the other treatments with 5 or 10 μM Fe in the nutrient solution”
Claim · model-written summary
Relative FC-R mRNA and shoot Fe plus Cu followed the same treatment pattern.
Verbatim
e0“The same pattern was obtained for relative FC-R mRNA concentration and for the sum of Fe and Cu contents in shoots (leaves plus crowns)”
Claim · model-written summary
Sequenced FC-R inserts were very similar despite different Fe20 SSCP patterns.
Verbatim
e0“Although there were different SSCP patterns in the Fe20 treatment, all the inserts that were sequenced were very similar”
Claim · model-written summary
The authors infer copper and iron regulate FC-R expression and activity.
Verbatim
e0“Cu as well as Fe is involved in FC-R expression and activity”
Claim · model-written summary
Low iron and copper contents led to FC-R gene overexpression and increased activity.
- Taxon
- strawberry
_single2.jpg?width=60)
Fragaria ×ananassae0 - Gene
- FC-R genee0
- Outcome
- over-expression of the FC-R gene and enhanced FC-R activitye0
- Qualifier
- small contents of Fe and Cue0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“Both small contents of Fe and Cu in plants led to an over-expression of the FC-R gene and enhanced FC-R activity in strawberry roots”
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This paper is about lacking the nutrient, so it counts the other way round here.
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See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.