Abstract
The "maize white spot" foliar disease is a problem of increasing importance to Brazilian maize crops. A bacterium isolated from water-soaked lesions from infected maize leaves was pathogenic in biological assays in vivo. It was identified as a Gram-negative, nonsporulating, facultative anaerobic bacterium, belonging to the genus Pantoea. Chemical study of the extracts from bacterial cultivation media allowed the identification of (S)-(-)-N-butanoyl-homoserine lactone and trace amounts of N-hexanoyl-homoserine lactone, widely recognized quorum-sensing signaling substances employed in cell-to-cell communication systems. The absolute configuration of natural (S)-(-)-N-butanoyl-homoserine lactone was determined by gas chromatography-flame ionization detection with a chiral stationary phase and by comparison of circular dichroism spectroscopic data with enantiopure synthetic substances. Biological evaluations with reporter Agrobacterium tumefaciens NTL4(pZLR4) were carried out with synthetic and natural products and also with extracts from maize leaves contaminated with the isolated bacterium, as well as from healthy leaves.
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Claims 5
Claim · model-written summary
A bacterium from infected maize leaves was pathogenic in in vivo assays.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A bacterium isolated from water-soaked lesions from infected maize leaves was pathogenic in biological assays in vivo.”
Claim · model-written summary
The maize lesion bacterium was identified as Pantoea.
- Outcome
- belonging to the genus Pantoeae0
.jpg?width=60){kind=small}
PantoeaOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
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Verbatim
e0“It was identified as a Gram-negative, nonsporulating, facultative anaerobic bacterium, belonging to the genus Pantoea.”
t0“Maize White Spot”
Claim · model-written summary
Cultures of the maize associated bacterium contained (S)-(-)-N-butanoyl-homoserine lactone.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chemical study of the extracts from bacterial cultivation media allowed the identification of (S)-(-)-N-butanoyl-homoserine lactone and trace amounts of N-hexanoyl-homoserine lactone”
t0“Maize White Spot”
Claim · model-written summary
Cultures of the maize associated bacterium contained trace N-hexanoyl-homoserine lactone.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chemical study of the extracts from bacterial cultivation media allowed the identification of (S)-(-)-N-butanoyl-homoserine lactone and trace amounts of N-hexanoyl-homoserine lactone”
t0“Maize White Spot”
Claim · model-written summary
The natural butanoyl homoserine lactone had the S configuration.
- Outcome
- absolute configuration of natural (S)-(-)-N-butanoyl-homoserine lactonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The absolute configuration of natural (S)-(-)-N-butanoyl-homoserine lactone was determined by gas chromatography-flame ionization detection with a chiral stationary phase and by comparison of circular dichroism spectroscopic data with enantiopure synthetic substances.”
t0“Maize White Spot”