Abstract
ABSTRACT The phytase (EC 3.1.3.8.) of Sanilac Navy Beans was extracted with 2% CaCl 2 and purified by ammonium sulfate fractionation and DEAE‐cellulose chromatography. The enzyme showed an optimum pH of 5.3 and Km of 0.018 mM with phytic acid as substrate. The optimum temperature was 50° C. The activation energy of the enzymic hydrolysis of phytic acid was 11,500 Cal/mole and the inactivation energy of the enzyme 55,800 Cal/mole. The purified phytase showed broad specificity. This enzyme may be described as a nonspecific phosphomonoesterase with phytase and potent pyrophosphatase activities. It was inhibited by high concentrations of phytic acid. The activity was increased by about 35% in the presence of 1 mM Co ++ . Soaking of beans in distilled water did not affect their phytic acid content and phytase activity. Germination of the beans resulted in an increase in phytase activity and breakdown of phytic acid.
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Claims 12
Claim · model-written summary
Purified navy bean phytase had a pH optimum of 5.3.
Verbatim
e0“The enzyme showed an optimum pH of 5.3”
t0“NAVY BEANS”
Claim · model-written summary
Phytase had a Km of 0.018 mM with phytic acid.
Verbatim
e0“Km of 0.018 mM with phytic acid as substrate”
t0“NAVY BEANS”
Claim · model-written summary
Phytase had an optimum temperature of 50° C.
Verbatim
e0“The optimum temperature was 50° C”
t0“NAVY BEANS”
Claim · model-written summary
Phytic acid hydrolysis had activation energy of 11,500 Cal/mole.
Verbatim
e0“The activation energy of the enzymic hydrolysis of phytic acid was 11,500 Cal/mole”
t0“NAVY BEANS”
Claim · model-written summary
Phytase inactivation energy was 55,800 Cal/mole.
Verbatim
e0“the inactivation energy of the enzyme 55,800 Cal/mole”
t0“NAVY BEANS”
Claim · model-written summary
Purified phytase had broad specificity and pyrophosphatase activity.
Verbatim
e0“The purified phytase showed broad specificity. This enzyme may be described as a nonspecific phosphomonoesterase with phytase and potent pyrophosphatase activities”
t0“NAVY BEANS”
Claim · model-written summary
High phytic acid concentrations inhibited phytase.
Not reportedComparator
Verbatim
e0“The purified phytase showed broad specificity. This enzyme may be described as a nonspecific phosphomonoesterase with phytase and potent pyrophosphatase activities. It was inhibited by high concentrations of phytic acid”
t0“NAVY BEANS”
Claim · model-written summary
One millimolar cobalt increased phytase activity by about 35%.
Not reportedComparator
Verbatim
e0“The activity was increased by about 35% in the presence of 1 mM Co ++”
t0“NAVY BEANS”
Claim · model-written summary
Soaking beans in distilled water did not alter phytic acid content.
- Taxon
- beanse0
- Treatment
- Soaking of beans in distilled watere0
- Outcome
- phytic acid contente0
- Direction
- did not affecte0
Not reportedComparator
Verbatim
e0“Soaking of beans in distilled water did not affect their phytic acid content and phytase activity”
Claim · model-written summary
Soaking beans in distilled water did not alter phytase activity.
- Taxon
- beanse0
- Treatment
- Soaking of beans in distilled watere0
- Outcome
- phytase activitye0
- Direction
- did not affecte0
Not reportedComparator
Verbatim
e0“Soaking of beans in distilled water did not affect their phytic acid content and phytase activity”
Claim · model-written summary
Germination increased bean phytase activity.
Not reportedComparator
Verbatim
e0“Germination of the beans resulted in an increase in phytase activity and breakdown of phytic acid”
Claim · model-written summary
Germination broke down bean phytic acid.
Not reportedComparator
Verbatim
e0“Germination of the beans resulted in an increase in phytase activity and breakdown of phytic acid”
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