Abstract
Boron (B) is an essential element for plants, and B deficiency is a worldwide agricultural problem. In B-deficient areas, B is often supplied as fertilizer, but excess B can be toxic to both plants and animals. Generation of B deficiency-tolerant plants could reduce B fertilizer use. Improved fertility under B-limiting conditions in Arabidopsis thaliana by overexpression of BOR1, a B transporter, has been reported, but the root growth was not improved by the BOR1 overexpression. In this study, we report that enhanced expression of NIP5;1, a boric acid channel for efficient B uptake, resulted in improved root elongation under B-limiting conditions in A. thaliana. An NIP5;1 activation tag line, which has a T-DNA insertion with enhancer sequences near the NIP5;1 gene, showed improved root elongation under B limitation. We generated a construct which mimics the tag line: the cauliflower mosaic virus 35S RNA promoter was inserted at 1,357 bp upstream of the NIP5;1 transcription initiation site. Introduction of this construct into the nip5;1-1 mutant and the BOR1 overexpresser resulted in enhanced expression of NIP5;1 and improved root elongation under low B supply. Furthermore, one of the transgenic lines exhibited improved fertility and short-term B uptake. Our results demonstrate successful improvement of B deficiency tolerance and the potential of enhancing expression of a mineral nutrient channel gene to improve growth under nutrient-limiting conditions.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
NIP5;1 enhanced expression improved Arabidopsis root elongation under boron limitation.
- Taxon
- A. thaliana
Arabidopsis thalianae0e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enhanced expression of NIP5;1, a boric acid channel for efficient B uptake, resulted in improved root elongation under B-limiting conditions in A. thaliana”
e1“In this study, we report that enhanced expression of NIP5;1, a boric acid channel for efficient B uptake, resulted in improved root elongation under B-limiting conditions in A. thaliana.”
Claim · model-written summary
The NIP5;1 activation tag line had better root elongation under B limitation.
- Taxon
- A. thalianaArabidopsis thalianae0e1t0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An NIP5;1 activation tag line, which has a T-DNA insertion with enhancer sequences near the NIP5;1 gene, showed improved root elongation under B limitation.”
t0“under B-limiting conditions in A. thaliana.”
e1“In this study, we report that enhanced expression of NIP5;1, a boric acid channel for efficient B uptake, resulted in improved root elongation under B-limiting conditions in A. thaliana.”
Claim · model-written summary
Introducing the construct into nip5;1-1 enhanced NIP5;1 expression.
- Taxon
- A. thalianaArabidopsis thalianae0e1t0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Introduction of this construct into the nip5;1-1 mutant and the BOR1 overexpresser resulted in enhanced expression of NIP5;1 and improved root elongation under low B supply.”
t0“under B-limiting conditions in A. thaliana.”
e1“In this study, we report that enhanced expression of NIP5;1, a boric acid channel for efficient B uptake, resulted in improved root elongation under B-limiting conditions in A. thaliana.”
Claim · model-written summary
Introducing the construct into the BOR1 overexpresser enhanced NIP5;1 expression.
- Taxon
- A. thalianaArabidopsis thalianae0e1t0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Introduction of this construct into the nip5;1-1 mutant and the BOR1 overexpresser resulted in enhanced expression of NIP5;1 and improved root elongation under low B supply.”
t0“under B-limiting conditions in A. thaliana.”
e1“In this study, we report that enhanced expression of NIP5;1, a boric acid channel for efficient B uptake, resulted in improved root elongation under B-limiting conditions in A. thaliana.”
Claim · model-written summary
Introducing the construct into nip5;1-1 improved root elongation under low B.
- Taxon
- A. thalianaArabidopsis thalianae0e1t0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Introduction of this construct into the nip5;1-1 mutant and the BOR1 overexpresser resulted in enhanced expression of NIP5;1 and improved root elongation under low B supply.”
t0“under B-limiting conditions in A. thaliana.”
e1“In this study, we report that enhanced expression of NIP5;1, a boric acid channel for efficient B uptake, resulted in improved root elongation under B-limiting conditions in A. thaliana.”
Claim · model-written summary
Introducing the construct into the BOR1 overexpresser improved root elongation under low B.
- Taxon
- A. thalianaArabidopsis thalianae0e1t0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Introduction of this construct into the nip5;1-1 mutant and the BOR1 overexpresser resulted in enhanced expression of NIP5;1 and improved root elongation under low B supply.”
t0“under B-limiting conditions in A. thaliana.”
e1“In this study, we report that enhanced expression of NIP5;1, a boric acid channel for efficient B uptake, resulted in improved root elongation under B-limiting conditions in A. thaliana.”
Claim · model-written summary
One transgenic line had improved fertility.
- Taxon
- A. thalianaArabidopsis thalianae0e1t0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Furthermore, one of the transgenic lines exhibited improved fertility and short-term B uptake.”
t0“under B-limiting conditions in A. thaliana.”
e1“In this study, we report that enhanced expression of NIP5;1, a boric acid channel for efficient B uptake, resulted in improved root elongation under B-limiting conditions in A. thaliana.”
Claim · model-written summary
One transgenic line had improved short-term boron uptake.
- Taxon
- A. thalianaArabidopsis thalianae0e1t0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Furthermore, one of the transgenic lines exhibited improved fertility and short-term B uptake.”
t0“under B-limiting conditions in A. thaliana.”
e1“In this study, we report that enhanced expression of NIP5;1, a boric acid channel for efficient B uptake, resulted in improved root elongation under B-limiting conditions in A. thaliana.”
The same questions in other papers
transgenic · growth · Arabidopsis thaliana (thale cress)Positive and negative results
19 papers in the same direction as this paper · 16 in the opposite direction
- negative
- no effect
- negative
32 papers more in this group
See all 36 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.