Abstract
An efficient in vitro propagation protocol for rapidly producing Cannabis sativa plantlets from young leaf tissue was developed. Using gas chromatography-flame ionization detection (GC-FID), high THC yielding elite female clone of a drug-type CANNABIS variety (MX) was screened and its vegetatively propagated clones were used for micropropagation. Calli were induced from leaf explant on Murashige and Skoog medium supplemented with different concentrations (0.5, 1.0, 1.5, and 2.0 µM) of indole- 3-acetic acid (IAA), indole- 3- butyric acid (IBA), naphthalene acetic acid (NAA), and 2,4-dichlorophenoxy-acetic acid (2,4-D) in combination with 1.0 µM of thidiazuron (TDZ) for the production of callus. The optimum callus growth and maintenance was in 0.5 µM NAA plus 1.0 µM TDZ. The two-month-old calli were subcultured to MS media containing different concentrations of cytokinins (BAP, KN, TDZ). The rate of shoot induction and proliferation was highest in 0.5 µM TDZ. Of the various auxins (IAA, IBA, and NAA) tested, regenerated shoots rooted best on half strength MS medium (1/2 - MS) supplemented with 2.5 µM IBA. The rooted plantlets were successfully established in soil and grown to maturity with no gross variations in morphology and cannabinoids content at a survival rate of 95 % in the indoor growroom.
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Claims 5
Claim · model-written summary
Callus growth and maintenance of Cannabis sativa leaf explants was optimum in 0.5 µM NAA plus 1.0 µM TDZ.
- Taxon
- Cannabis sativa
Cannabis sativae0 - Organ / stage
- leaf explante1
- Treatment
- 0.5 µM NAA plus 1.0 µM TDZe2
- Outcome
- callus growth and maintenancee2
- Direction
- optimume2
- Comparator
- different concentrations (0.5, 1.0, 1.5, and 2.0 µM)e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“producing Cannabis sativa plantlets from young leaf tissue”
e1“Calli were induced from leaf explant on Murashige and Skoog medium supplemented with different concentrations (0.5, 1.0, 1.5, and 2.0 µM) of indole- 3-acetic acid (IAA)”
e2“The optimum callus growth and maintenance was in 0.5 µM NAA plus 1.0 µM TDZ”
Claim · model-written summary
The rate of Cannabis sativa shoot induction and proliferation was highest in 0.5 µM TDZ.
- Taxon
- Cannabis sativaCannabis sativae0
- Organ / stage
- two-month-old callie3
- Treatment
- 0.5 µM TDZe4
- Outcome
- rate of shoot induction and proliferatione4
- Direction
- higheste4
- Comparator
- different concentrations of cytokinins (BAP, KN, TDZ)e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“producing Cannabis sativa plantlets from young leaf tissue”
e3“The two-month-old calli were subcultured to MS media containing different concentrations of cytokinins (BAP, KN, TDZ)”
e4“The rate of shoot induction and proliferation was highest in 0.5 µM TDZ”
Claim · model-written summary
Regenerated Cannabis sativa shoots rooted best on half strength MS medium with 2.5 µM IBA among the auxins tested.
- Taxon
- Cannabis sativaCannabis sativae0
- Organ / stage
- regenerated shootse5
- Treatment
- 2.5 µM IBAe5
- Outcome
- rootede5
- Direction
- beste5
- Comparator
- various auxins (IAA, IBA, and NAA)e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“producing Cannabis sativa plantlets from young leaf tissue”
e5“Of the various auxins (IAA, IBA, and NAA) tested, regenerated shoots rooted best on half strength MS medium (1/2 - MS) supplemented with 2.5 µM IBA”
Claim · model-written summary
Rooted Cannabis sativa plantlets established in soil had a survival rate of 95 % in the indoor growroom.
- Taxon
- Cannabis sativaCannabis sativae0
- Organ / stage
- rooted plantletse6
- Outcome
- survival ratee6
- Value
- 95 %e6
- Setting
- indoor growroome6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“producing Cannabis sativa plantlets from young leaf tissue”
e6“The rooted plantlets were successfully established in soil and grown to maturity with no gross variations in morphology and cannabinoids content at a survival rate of 95 % in the indoor growroom”
Claim · model-written summary
Rooted Cannabis sativa plantlets grown to maturity showed no gross variation in morphology or cannabinoids content.
- Taxon
- Cannabis sativaCannabis sativae0
- Outcome
- morphology and cannabinoids contente6
- Qualifier
- no gross variationse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“producing Cannabis sativa plantlets from young leaf tissue”
e6“The rooted plantlets were successfully established in soil and grown to maturity with no gross variations in morphology and cannabinoids content at a survival rate of 95 % in the indoor growroom”
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See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.