Abstract
We have purified a cell wall protein from extracts of soybean (Glycine max) that was previously shown to be immunologically related to p33, a wound-induced carrot cell wall protein (Tierney ML, Wiechert J, Pluymers D [1988] Mol Gen Genet 211: 393-399). Amino acid composition analysis reveals that the protein is 36% proline and hydroxyproline and its amino-terminus was determined to be AsnTyrGluAsnProHypValTyrLysProHypThrGluLysProHypValTyr. In vivo [(3)H]proline labeling of soybean hypocotyl tissues and Western blot analysis indicate that the apical hook, a region characterized by active growth, is a predominant site for synthesis of this protein and that wounding does not significantly affect its synthesis or accumulation. Antibodies raised against the hook protein also cross-reacted with a higher molecular weight protein in cell wall extracts of wounded hook tissue. These data indicate that there are at least two related, proline-rich cell wall proteins in the hook region of soybean seedlings, one that appears to be developmentally regulated and another that is wound-inducible. RNA encoding the developmentally regulated seedling cell wall protein was detected in both the hook and elongating region of the hypocotyl. The presence of this RNA in the elongating region in the absence of detectable levels of the protein indicates either that the expression of this cell wall protein is subject to posttranscriptional control or that the rate of insolubilization of this protein increases in more mature regions of the hypocotyl.
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Claims 8
Claim · model-written summary
The purified soybean cell wall protein contains 36% proline and hydroxyproline.
- Taxon
- soybean
Glycine maxe1 - Outcome
- proline and hydroxyprolinee0
- Value
- 36%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the protein is 36% proline and hydroxyproline”
e1“soybean (Glycine max)”
Claim · model-written summary
The protein amino terminus was identified as AsnTyrGluAsnProHypValTyrLysProHypThrGluLysProHypValTyr.
- Taxon
- soybeanGlycine maxe1
- Outcome
- amino-terminuse0
Not reportedValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its amino-terminus was determined to be AsnTyrGluAsnProHypValTyrLysProHypThrGluLysProHypValTyr”
e1“soybean (Glycine max)”
Claim · model-written summary
The apical hook was the predominant synthesis site of this protein.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- apical hooke0
- Outcome
- synthesis of this proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the apical hook, a region characterized by active growth, is a predominant site for synthesis of this protein”
e1“soybean hypocotyl tissues”
Claim · model-written summary
Wounding did not significantly affect protein synthesis or accumulation.
- Taxon
- soybeanGlycine maxe1
- Treatment
- woundinge0
- Outcome
- synthesis or accumulatione0
- Direction
- does not significantly affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“wounding does not significantly affect its synthesis or accumulation”
e1“soybean hypocotyl tissues”
Claim · model-written summary
Hook protein antibodies cross reacted with a higher molecular weight protein in wounded hook tissue.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- wounded hook tissuee0
- Outcome
- higher molecular weight proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antibodies raised against the hook protein also cross-reacted with a higher molecular weight protein in cell wall extracts of wounded hook tissue”
e1“soybean hypocotyl tissues”
Claim · model-written summary
Two related proline rich proteins occur in the soybean seedling hook, one developmentally regulated and one wound inducible.
- Taxon
- soybeanGlycine maxe0
- Organ / stage
- hook regione0
- Outcome
- at least two related, proline-rich cell wall proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there are at least two related, proline-rich cell wall proteins in the hook region of soybean seedlings, one that appears to be developmentally regulated and another that is wound-inducible”
Claim · model-written summary
RNA encoding the developmentally regulated protein was detected in the hook and elongating hypocotyl.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- hook and elongating region of the hypocotyle0
- Outcome
- RNA encoding the developmentally regulated seedling cell wall proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA encoding the developmentally regulated seedling cell wall protein was detected in both the hook and elongating region of the hypocotyl”
e1“soybean hypocotyl tissues”
Claim · model-written summary
RNA was present without detectable protein in the elongating region, consistent with posttranscriptional control or faster protein insolubilization.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- elongating regione0
- Outcome
- absence of detectable levels of the proteine0
- Qualifier
- posttranscriptional controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The presence of this RNA in the elongating region in the absence of detectable levels of the protein indicates either that the expression of this cell wall protein is subject to posttranscriptional control or that the rate of insolubilization of this protein increases in more mature regions of the hypocotyl”
e1“soybean hypocotyl tissues”
The same questions in other papers
mechanical · molecular: metabolites · Glycine max (soybean)Positive and negative results
3 papers in the same direction as this paper · 5 in the opposite direction
- positive
- positive
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.