Abstract
A 2-step, 1-dimensional thin-layer chromatographic procedure for isolating indoleacetic acid (IAA) was developed and utilized in investigations of the biosynthesis of IAA from tryptophan-(14)C in cell-free extracts of pea (Pisum sativum L.) shoot tips. Identification of a (14)C-product as IAA was by (a) co-chromatography of authentic IAA and (14)C-product on thin-layer chromatography, and (b) gas-liquid and thin-layer chromatography of authentic and presumptive IAA methyl esters. Dialysis of enzyme extracts and addition of alpha-ketoglutaric acid and pyridoxal phosphate to reaction mixtures resulted in approximately 2- to 3-fold increases in net yields of IAA over yields in non-dialyzed reaction mixtures which did not contain additives essential to a transaminase reaction of tryptophan. Addition of thiamine pyrophosphate to reaction mixtures further enhanced net biosynthesis of IAA. It is concluded that the formation of indolepyruvic acid and its subsequent decarboxylation probably are sequential reactions in the major pathway of IAA biosynthesis from tryptophan in cell-free extracts of Pisum shoot tips. Comparison of maximum net IAA biosynthesis in extracts of shoot tips of etiolated and light-grown dwarf and tall pea seedlings revealed an order, on a unit protein N basis, of: light-grown tall > light-grown dwarf > etiolated tall congruent with etiolated dwarf. It is concluded that the different rates of stem elongation among etiolated and light-grown dwarf and tall pea seedlings are correlated, in general, with differences in net IAA biosynthesis and sensitivity of the tissues to IAA.
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Claims 7
Claim · model-written summary
A two-step one-dimensional thin-layer chromatographic procedure was developed to isolate IAA for pea shoot-tip biosynthesis studies.
- Taxon
- Pisum sativum L.
Lathyrus oleraceuse0 - Outcome
- procedure for isolating indoleacetic acid (IAA)e0
- Qualifier
- 2-step, 1-dimensional thin-layer chromatographice0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A 2-step, 1-dimensional thin-layer chromatographic procedure for isolating indoleacetic acid (IAA) was developed and utilized in investigations of the biosynthesis of IAA from tryptophan-(14)C in cell-free extracts of pea (Pisum sativum L.) shoot tips.”
Claim · model-written summary
A labelled product from pea extracts was identified as IAA by chromatographic comparisons.
- Taxon
- Pisum sativum L.Lathyrus oleraceuse1
- Outcome
- (14)C-product as IAAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Identification of a (14)C-product as IAA was by (a) co-chromatography of authentic IAA and (14)C-product on thin-layer chromatography, and (b) gas-liquid and thin-layer chromatography of authentic and presumptive IAA methyl esters.”
e1“pea (Pisum sativum L.) shoot tips”
Claim · model-written summary
Dialysis plus alpha-ketoglutaric acid and pyridoxal phosphate increased net IAA yield about two- to threefold over non-dialyzed mixtures without additives.
- Taxon
- Pisum sativum L.Lathyrus oleraceuse1
- Treatment
- Dialysis of enzyme extracts and addition of alpha-ketoglutaric acid and pyridoxal phosphatee0
- Outcome
- net yields of IAAe0
- Direction
- increasese0
- Value
- approximately 2- to 3-folde0
- Qualifier
- which did not contain additives essential to a transaminase reaction of tryptophane0
- Comparator
- non-dialyzed reaction mixturese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Dialysis of enzyme extracts and addition of alpha-ketoglutaric acid and pyridoxal phosphate to reaction mixtures resulted in approximately 2- to 3-fold increases in net yields of IAA over yields in non-dialyzed reaction mixtures which did not contain additives essential to a transaminase reaction of tryptophan.”
e1“pea (Pisum sativum L.) shoot tips”
Claim · model-written summary
Thiamine pyrophosphate further enhanced net IAA biosynthesis in pea extracts.
- Taxon
- Pisum sativum L.Lathyrus oleraceuse1
- Treatment
- Addition of thiamine pyrophosphatee0
- Outcome
- net biosynthesis of IAAe0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of thiamine pyrophosphate to reaction mixtures further enhanced net biosynthesis of IAA.”
e1“pea (Pisum sativum L.) shoot tips”
Claim · model-written summary
Indolepyruvic acid formation and subsequent decarboxylation probably form sequential reactions in the major IAA biosynthetic pathway from tryptophan in pea extracts.
- Organ / stage
- shoot tipse0
- Compound
- indolepyruvic acide0
- Outcome
- IAA biosynthesis from tryptophane0
- Qualifier
- probably are sequential reactionse0
LathyrusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It is concluded that the formation of indolepyruvic acid and its subsequent decarboxylation probably are sequential reactions in the major pathway of IAA biosynthesis from tryptophan in cell-free extracts of Pisum shoot tips.”
Claim · model-written summary
Maximum net IAA biosynthesis per unit protein N ranked light-grown tall above light-grown dwarf above approximately equal etiolated tall and dwarf pea seedlings.
- Taxon
- peaLathyrus oleraceuse0
- Outcome
- maximum net IAA biosynthesise0
- Direction
- higherinferrede0
- Qualifier
- light-grown tall > light-grown dwarf > etiolated tall congruent with etiolated dwarfe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Comparison of maximum net IAA biosynthesis in extracts of shoot tips of etiolated and light-grown dwarf and tall pea seedlings revealed an order, on a unit protein N basis, of: light-grown tall > light-grown dwarf > etiolated tall congruent with etiolated dwarf.”
Claim · model-written summary
Stem elongation differences among pea seedlings generally correlated with net IAA biosynthesis and tissue sensitivity to IAA.
- Taxon
- peaLathyrus oleraceuse0
- Compound
- IAAe0
- Outcome
- rates of stem elongatione0
- Qualifier
- in generale0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It is concluded that the different rates of stem elongation among etiolated and light-grown dwarf and tall pea seedlings are correlated, in general, with differences in net IAA biosynthesis and sensitivity of the tissues to IAA.”
The same questions in other papers
botanical · yield · Lathyrus oleraceus (pea)Positive and negative results
24 papers in the same direction as this paper · 1 reporting no difference from an alternative
- no effect
- positive
- positive
22 papers more in this group
See all 26 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.