Paper · 1986 · Journal of Experimental Botany

Changing Kinetic Properties of Barley Alcohol Dehydrogenase during Hypoxic Conditions

K. V. FAGERSTEDT; R. M. M. CRAWFORD

6
claims
13
verbatim quotes
11
citations
2
research areas
—
Not open access

Abstract

The Finnish barley cultivar (Hordeum vulgare L, cv. Hankkija-673) was grown in solution culture for periods of one and two months. The one month old plants had seminal roots, whereas the two month old plants grew adventitious roots from the lower nodes. The roots were tested for ADH activity during aeration, during 3 d of hypoxic treatment (brought about by passing nitrogen through the nutrient solution), and during a 4 d recovery period. The ADH activity, calculated on a protein basis, rose about 4-fold during the nitrogen treatment, the greatest increase occurring in the adventitious roots. Differences in the kinetic properties of ADH during the hypoxic period were also found between seminal and adventitious roots. The Km for acetaldehyde changed little in the seminal roots during the hypoxic treatment, but in the adventitious roots it decreased considerably. The physiological significance of these changes is discussed.

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Claims 6

Observationobservedoriginal resultmeasured

Claim · model-written summary

Two month old barley developed adventitious roots from lower nodes.

Organ / stage
two month old plantse0
Outcome
grew adventitious roots from the lower nodese0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the two month old plants grew adventitious roots from the lower nodes”

✓ located verbatim in the source · abstract, characters 183–252 · show in text ↑
t0

“Barley”

✓ located verbatim in the source · title, characters 31–37 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Nitrogen treatment increased root ADH activity about fourfold.

Treatment
nitrogen treatmente0
Gene
ADHe0
Outcome
ADH activitye0
Direction
rosee0
Value
about 4-folde0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The ADH activity, calculated on a protein basis, rose about 4-fold during the nitrogen treatment, the greatest increase occurring in the adventitious roots.”

✓ located verbatim in the source · abstract, characters 443–599 · show in text ↑
t0

“Barley”

✓ located verbatim in the source · title, characters 31–37 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

The largest ADH activity increase occurred in adventitious roots.

Organ / stage
adventitious rootse0
Outcome
increasee0
Direction
greatest increasee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the greatest increase occurring in the adventitious roots”

✓ located verbatim in the source · abstract, characters 541–598 · show in text ↑
t0

“Barley”

✓ located verbatim in the source · title, characters 31–37 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

ADH kinetic properties differed between seminal and adventitious roots during hypoxia.

Organ / stage
seminal and adventitious rootse0
Gene
ADHe0
Outcome
kinetic propertiese0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Differences in the kinetic properties of ADH during the hypoxic period were also found between seminal and adventitious roots.”

✓ located verbatim in the source · abstract, characters 600–726 · show in text ↑
t0

“Barley”

✓ located verbatim in the source · title, characters 31–37 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Acetaldehyde Km changed little in seminal roots during hypoxia.

Organ / stage
seminal rootse0
Treatment
hypoxic treatmente0
Outcome
Km for acetaldehydee0
Direction
changed littlee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The Km for acetaldehyde changed little in the seminal roots during the hypoxic treatment”

✓ located verbatim in the source · abstract, characters 727–815 · show in text ↑
t0

“Barley”

✓ located verbatim in the source · title, characters 31–37 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Acetaldehyde Km decreased considerably in adventitious roots during hypoxia.

Organ / stage
adventitious rootse0
Treatment
hypoxic treatmente1
Outcome
acetaldehydee1
Direction
decreasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“but in the adventitious roots it decreased considerably”

✓ located verbatim in the source · abstract, characters 817–872 · show in text ↑
t0

“Barley”

✓ located verbatim in the source · title, characters 31–37 · show in text ↑
e1

“The Km for acetaldehyde changed little in the seminal roots during the hypoxic treatment”

✓ located verbatim in the source · abstract, characters 727–815 · show in text ↑

flooding · molecular: metabolites · Hordeum vulgare (barley)Positive and negative results

12 papers: 4 positive, 8 negative or no effect

7 papers in the same direction as this paper · 4 in the opposite direction

  1. positive
  2. positive
  3. positive

8 papers more in this group

See all 12 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.