Abstract
Previous data in the literature have indicated that phytochrome could alter the rate of reduction of exogenously added NADP by a pea mitochondrial preparation in vitro. These results could not be duplicated using a mitochondrial preparation isolated from etiolated oat seedlings. Further experimentation demonstrated that the addition of Pr to the preparation, in combination with a far red light illumination, could significantly reduce the rate of oxidation of NADH by the external dehydrogenases of oat mitochondria. This response was characterized by a 15% decrease in reaction velocity at saturating substrate concentrations and a 2-fold increase in apparent K(m) as compared to values obtained after Pfr plus red light treatment. The response was photoreversible, the rate of oxidation of exogenous NADH being determined by the last light illumination given to the mitochondrial preparation. The interaction between phytochrome and the mitochondria was apparently occurring at the level of the inner mitochondrial membrane. A requirement for these results was that the mitochondria be isolated from plants that were illuminated with white or red light before extraction; mitochondria from unirradiated plants showed no dehydrogenase response to treatments with phytochrome plus actinic light.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
The earlier pea mitochondrial NADP reduction result could not be reproduced with oat mitochondria.
- Taxon
- oat seedlings
Avena sativae0 - Outcome
- results could not be duplicatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results could not be duplicated using a mitochondrial preparation isolated from etiolated oat seedlings.”
Claim · model-written summary
Pr and far red light significantly lowered oat mitochondrial NADH oxidation.
- Taxon
- oatAvena sativae0
- Treatment
- addition of Pr to the preparation, in combination with a far red light illuminatione0
- Gene
- external dehydrogenasese0
- Outcome
- rate of oxidation of NADHe0
- Direction
- reducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the addition of Pr to the preparation, in combination with a far red light illumination, could significantly reduce the rate of oxidation of NADH by the external dehydrogenases of oat mitochondria.”
Claim · model-written summary
The treatment reduced reaction velocity by 15% versus Pfr plus red light.
- Taxon
- oatAvena sativat0
- Treatment
- Pre1
- Outcome
- reaction velocitye0
- Direction
- decreasee0
- Value
- 15%e0
- Qualifier
- saturating substrate concentrationse0
- Comparator
- Pfr plus red light treatmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a 15% decrease in reaction velocity at saturating substrate concentrations and a 2-fold increase in apparent K(m) as compared to values obtained after Pfr plus red light treatment.”
t0“Oat”
e1“the addition of Pr to the preparation, in combination with a far red light illumination, could significantly reduce the rate of oxidation of NADH by the external dehydrogenases of oat mitochondria.”
Claim · model-written summary
The treatment doubled apparent K(m) versus Pfr plus red light.
- Taxon
- oatAvena sativat0
- Treatment
- Pre1
- Outcome
- apparent K(m)e0
- Direction
- increasee0
- Value
- 2-folde0
- Comparator
- Pfr plus red light treatmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a 15% decrease in reaction velocity at saturating substrate concentrations and a 2-fold increase in apparent K(m) as compared to values obtained after Pfr plus red light treatment.”
t0“Oat”
e1“the addition of Pr to the preparation, in combination with a far red light illumination, could significantly reduce the rate of oxidation of NADH by the external dehydrogenases of oat mitochondria.”
Claim · model-written summary
The mitochondrial NADH oxidation response was photoreversible.
- Taxon
- oatAvena sativat0
- Outcome
- response was photoreversiblee0
- Qualifier
- last light illuminatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The response was photoreversible, the rate of oxidation of exogenous NADH being determined by the last light illumination given to the mitochondrial preparation.”
t0“Oat”
Claim · model-written summary
Phytochrome appeared to interact with the inner mitochondrial membrane.
- Taxon
- oatAvena sativat0
- Outcome
- interaction between phytochrome and the mitochondriae0
- Qualifier
- inner mitochondrial membranee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The interaction between phytochrome and the mitochondria was apparently occurring at the level of the inner mitochondrial membrane.”
t0“Oat”
Claim · model-written summary
Mitochondria from unirradiated plants lacked a dehydrogenase response to phytochrome and light.
- Taxon
- oatAvena sativat0
- Outcome
- no dehydrogenase responsee0
- Qualifier
- unirradiated plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mitochondria from unirradiated plants showed no dehydrogenase response to treatments with phytochrome plus actinic light.”
t0“Oat”
The same questions in other papers
light · molecular: metabolites · Avena sativa (oats)Positive and negative results
4 papers in the same direction as this paper · 6 in the opposite direction
- positive
- positive
- positive
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.