Paper · 1967 · Canadian Journal of Botany

GROWTH AND DEVELOPMENT OF POTATO CALLUS IN SUSPENSION CULTURES

Y. P. S. Bajaj; L. A. Dionne

6
claims
14
verbatim quotes
12
citations
1
research areas
—
Not open access

Abstract

Potato callus raised from placental tissue was continuously grown in agitated liquid cultures. Best growth of the callus and its dissociation into single cells and clumps of cells was obtained in a medium containing Murashige and Skoog's minerals +2,4-dichlorophenoxyacetic acid (0.2 p.p.m.) +α-naphthaleneacetic acid (0.2 p.p.m.) + yeast extract (1000 p.p.m.). When a suspension of cells from this medium was transferred to fresh medium of the same composition, the clumps of cells proliferated further to form masses of callus. Occasionally, differentiation of roots from the callus was also observed. In media containing kinetin (0.2 p.p.m.) or 6(γ,γ-dimethylallylamino)-purine, large numbers of shiny, rounded pearl-like callus masses were obtained. The development of these callus bodies and their subsequent growth proved better when the kinin used in the medium was 6(γ,γ-dimethylallylamino)-purine.

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The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 6

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Best growth of potato callus and its dissociation into single cells and clumps was obtained in the medium with yeast extract (1000 p.p.m.).

Treatment
yeast extract (1000 p.p.m.)e3
Outcome
growth of the calluse2
Qualifier
dissociation into single cells and clumps of cellse2

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e1

“Potato callus raised from placental tissue was continuously grown in agitated liquid cultures”

✓ located verbatim in the source · abstract, characters 0–93 · show in text ↑
e2

“Best growth of the callus and its dissociation into single cells and clumps of cells was obtained”

✓ located verbatim in the source · abstract, characters 95–192 · show in text ↑
e3

“yeast extract (1000 p.p.m.)”

✓ located verbatim in the source · abstract, characters 333–360 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Clumps of potato callus cells proliferated further to form masses of callus after transfer to fresh medium.

Outcome
clumps of cells proliferated furthere4

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e1

“Potato callus raised from placental tissue was continuously grown in agitated liquid cultures”

✓ located verbatim in the source · abstract, characters 0–93 · show in text ↑
e4

“the clumps of cells proliferated further to form masses of callus”

✓ located verbatim in the source · abstract, characters 463–528 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Roots occasionally differentiated from the potato callus.

Organ / stage
rootse5
Outcome
differentiation of roots from the calluse5

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e1

“Potato callus raised from placental tissue was continuously grown in agitated liquid cultures”

✓ located verbatim in the source · abstract, characters 0–93 · show in text ↑
e5

“Occasionally, differentiation of roots from the callus was also observed”

✓ located verbatim in the source · abstract, characters 530–602 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Kinetin (0.2 p.p.m.) gave large numbers of shiny, rounded pearl-like potato callus masses.

Treatment
kinetin (0.2 p.p.m.)e6
Outcome
shiny, rounded pearl-like callus massese6

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e1

“Potato callus raised from placental tissue was continuously grown in agitated liquid cultures”

✓ located verbatim in the source · abstract, characters 0–93 · show in text ↑
e6

“In media containing kinetin (0.2 p.p.m.) or 6(γ,γ-dimethylallylamino)-purine, large numbers of shiny, rounded pearl-like callus masses were obtained”

✓ located verbatim in the source · abstract, characters 604–752 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

6(γ,γ-dimethylallylamino)-purine gave large numbers of shiny, rounded pearl-like potato callus masses.

Treatment
6(γ,γ-dimethylallylamino)-purinee6
Outcome
shiny, rounded pearl-like callus massese6

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e1

“Potato callus raised from placental tissue was continuously grown in agitated liquid cultures”

✓ located verbatim in the source · abstract, characters 0–93 · show in text ↑
e6

“In media containing kinetin (0.2 p.p.m.) or 6(γ,γ-dimethylallylamino)-purine, large numbers of shiny, rounded pearl-like callus masses were obtained”

✓ located verbatim in the source · abstract, characters 604–752 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Development and subsequent growth of potato callus bodies were better with 6(γ,γ-dimethylallylamino)-purine than with kinetin.

Treatment
6(γ,γ-dimethylallylamino)-purinee7
Outcome
development of these callus bodiese7
Direction
bettere7
Comparator
kinetine6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e1

“Potato callus raised from placental tissue was continuously grown in agitated liquid cultures”

✓ located verbatim in the source · abstract, characters 0–93 · show in text ↑
e6

“In media containing kinetin (0.2 p.p.m.) or 6(γ,γ-dimethylallylamino)-purine, large numbers of shiny, rounded pearl-like callus masses were obtained”

✓ located verbatim in the source · abstract, characters 604–752 · show in text ↑
e7

“The development of these callus bodies and their subsequent growth proved better when the kinin used in the medium was 6(γ,γ-dimethylallylamino)-purine”

✓ located verbatim in the source · abstract, characters 754–905 · show in text ↑

kinetin · growth · Solanum tuberosum (potato)Positive and negative results

15 papers: 12 positive, 3 negative or no effect

11 papers in the same direction as this paper · 3 in the opposite direction

  1. negative
  2. no effect
  3. no effect

11 papers more in this group

See all 15 papers →

botanical · growth · Solanum tuberosum (potato)Positive and negative results

35 papers: 30 positive, 5 negative or no effect

29 papers in the same direction as this paper · 5 in the opposite direction

  1. negative
  2. no effect
  3. negative

31 papers more in this group

See all 35 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.