Abstract
ABSTRACT In the pea plant ( Pisum sativum ), compounds that intercalate into DNA induce the production of ∼20 major proteins similar to the pattern induced during nonhost disease resistance to the bean fungal pathogen, Fusarium solani f.sp. phaseoli . The pea phytoalexin, pisatin, as well as RNA homologous to several disease‐resistance response (DRR) genes accumulate following treatment with these compounds. Psoralen was chosen to characterize this interaction further because it intercalates into DNA and, following irradiation with 365 nm UV light (UV 365 ), forms covalent bonds with pyrimidines on either or both strands of DNA. This produces monoadducts or cross‐links, respectively. Dose experiments showed that 60 μg/mL 4′‐aminomethyl‐4,5′,8‐trimethylpsoralen followed by 18 J/cm 2 UV 365 was sufficient to produce an accumulation of pisatin similar to that produced in response to the fungus. Under these inducing conditions, there was an average of 0.19 adducts per kb of pea genomic DNA. The accumulation of pisatin and the RNA of several DRR genes by psoralen required photoactivation, which suggests that covalent binding to DNA was necessary for induction. As the promoters of several putative fungal‐induced pea genes contain long stretches of d(AT) n , which is the preferred psoralen photobinding site, restriction fragments spanning DRR genes were examined after in vivo psoralen treatment. The rate of crosslinking was compared between fungal‐induced and noninduced genes using a modified Southern blot analysis. Implications of the induction of the DRR due to psoralen binding are discussed.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
DNA-intercalating compounds induce about 20 major proteins in pea, similar to the pattern of nonhost disease resistance.
- Taxon
- pea plant
Lathyrus oleraceuse1 - Treatment
- compounds that intercalate into DNAe1
- Interactor
- Fusarium solani f.sp. phaseoliFusarium phaseolie1pathogen
- Outcome
- major proteinse1
- Direction
- inducee1
- Comparator
- nonhost disease resistancee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In the pea plant ( Pisum sativum ), compounds that intercalate into DNA induce the production of ∼20 major proteins similar to the pattern induced during nonhost disease resistance to the bean fungal pathogen, Fusarium solani f.sp. phaseoli”
Claim · model-written summary
A psoralen dose with UV 365 nm exposure produced a pisatin accumulation similar to that after fungal challenge.
- Taxon
- pea plantLathyrus oleraceuse1
- Treatment
- 60 μg/mL 4′‐aminomethyl‐4,5′,8‐trimethylpsoralen followed by 18 J/cm 2 UV 365e2
- Outcome
- accumulation of pisatine2
- Direction
- similare2
- Comparator
- in response to the funguse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In the pea plant ( Pisum sativum ), compounds that intercalate into DNA induce the production of ∼20 major proteins similar to the pattern induced during nonhost disease resistance to the bean fungal pathogen, Fusarium solani f.sp. phaseoli”
e2“Dose experiments showed that 60 μg/mL 4′‐aminomethyl‐4,5′,8‐trimethylpsoralen followed by 18 J/cm 2 UV 365 was sufficient to produce an accumulation of pisatin similar to that produced in response to the fungus”
Claim · model-written summary
Psoralen treatment gave an average of 0.19 DNA adducts per kb of pea genomic DNA.
- Taxon
- peaLathyrus oleraceuse3
- Outcome
- adductse3
- Value
- 0.19 adducts per kbe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Under these inducing conditions, there was an average of 0.19 adducts per kb of pea genomic DNA”
Claim · model-written summary
Accumulation of pisatin and DRR gene RNA by psoralen required photoactivation.
- Taxon
- pea plantLathyrus oleraceuse1
- Treatment
- psoralene4
- Outcome
- accumulation of pisatine4
- Direction
- requirede4
- Qualifier
- photoactivatione4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In the pea plant ( Pisum sativum ), compounds that intercalate into DNA induce the production of ∼20 major proteins similar to the pattern induced during nonhost disease resistance to the bean fungal pathogen, Fusarium solani f.sp. phaseoli”
e4“The accumulation of pisatin and the RNA of several DRR genes by psoralen required photoactivation”
Claim · model-written summary
Covalent binding of psoralen to DNA is suggested to be necessary for the induction.
- Taxon
- pea plantLathyrus oleraceuse1
- Outcome
- inductione5
- Qualifier
- covalent binding to DNAe5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In the pea plant ( Pisum sativum ), compounds that intercalate into DNA induce the production of ∼20 major proteins similar to the pattern induced during nonhost disease resistance to the bean fungal pathogen, Fusarium solani f.sp. phaseoli”
e5“which suggests that covalent binding to DNA was necessary for induction”
Claim · model-written summary
Psoralen treatment with these compounds led to accumulation of RNA homologous to disease-resistance response genes in pea.
- Taxon
- peaLathyrus oleraceuse6
- Treatment
- these compoundse6
- Outcome
- RNA homologous to several disease‐resistance response (DRR) genese6
- Direction
- accumulatee6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“The pea phytoalexin, pisatin, as well as RNA homologous to several disease‐resistance response (DRR) genes accumulate following treatment with these compounds”
Claim · model-written summary
Pisatin accumulates in pea following treatment with these compounds.
- Taxon
- peaLathyrus oleraceuse6
- Treatment
- these compoundse6
- Outcome
- pisatine6
- Direction
- accumulatee6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“The pea phytoalexin, pisatin, as well as RNA homologous to several disease‐resistance response (DRR) genes accumulate following treatment with these compounds”
The same questions in other papers
uv · molecular: metabolites · Lathyrus oleraceus (pea)Positive and negative results
6 papers in the same direction as this paper · 11 in the opposite direction
- no effect
- no effect
- negative
14 papers more in this group
See all 18 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.