Abstract
Cultivar differences have been reported in the protoplast yields from Pisum sativum root cortical explants treated with preparations of commercial cellulase and pectinase. The presence of intracellular starch significantly influenced these protoplast yields. The application of gibberellin before or during the enzymic wall‐degradation increased the protoplast yields from two of the five cultivars tested. For tissues of‘Little Marvel’ pea roots, 10 mg 1 −1 of gibberellin most effectively increased the release of protoplasts when the hormone preceded the enzyme incubation. One mg 1 −1 of gibberellin was most effective at increasing the protoplast release when the tissues were treated with the hormone simultaneously with the wall‐degrading enzymes. Mitotic activity was significantly reduced in protoplasts derived from gibberellin‐treated tissues.
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Claims 5
Claim · model-written summary
Intracellular starch significantly influenced pea root protoplast yields.
- Taxon
- Pisum sativum
Lathyrus oleraceuse1 - Organ / stage
- root cortical explantse1
- Compound
- intracellular starche0
- Outcome
- protoplast yieldse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The presence of intracellular starch significantly influenced these protoplast yields”
e1“Pisum sativum root cortical explants”
Claim · model-written summary
Gibberellin applied before or during wall degradation increased protoplast yields in two of five pea cultivars.
- Taxon
- Pisum sativumLathyrus oleraceuse1
- Organ / stage
- root cortical explantse1
- Treatment
- application of gibberellin before or during the enzymic wall‐degradatione0
- Outcome
- protoplast yieldse0
- Direction
- increasede0
- Qualifier
- two of the five cultivars testede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The application of gibberellin before or during the enzymic wall‐degradation increased the protoplast yields from two of the five cultivars tested”
e1“Pisum sativum root cortical explants”
Claim · model-written summary
In Little Marvel pea roots, 10 mg 1 −1 gibberellin most effectively increased protoplast release when applied before enzyme incubation.
- Taxon
- peaLathyrus oleraceuse0
- Material
- Little Marvele0
- Organ / stage
- rootse0
- Treatment
- 10 mg 1 −1 of gibberelline0
- Outcome
- release of protoplastse0
- Direction
- most effectively increasede0
- Qualifier
- when the hormone preceded the enzyme incubatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“For tissues of‘Little Marvel’ pea roots, 10 mg 1 −1 of gibberellin most effectively increased the release of protoplasts when the hormone preceded the enzyme incubation”
Claim · model-written summary
One mg 1 −1 gibberellin most effectively increased protoplast release when applied simultaneously with wall-degrading enzymes.
- Taxon
- peaLathyrus oleraceuse1
- Material
- Little Marvele1
- Treatment
- One mg 1 −1 of gibberelline0
- Outcome
- protoplast releasee0
- Direction
- most effective at increasinge0
- Qualifier
- simultaneously with the wall‐degrading enzymese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One mg 1 −1 of gibberellin was most effective at increasing the protoplast release when the tissues were treated with the hormone simultaneously with the wall‐degrading enzymes”
e1“For tissues of‘Little Marvel’ pea roots”
Claim · model-written summary
Gibberellin treatment significantly reduced mitotic activity in derived protoplasts.
- Taxon
- Pisum sativumLathyrus oleraceuse1
- Organ / stage
- protoplastse0
- Treatment
- gibberellin‐treated tissuese0
- Outcome
- Mitotic activitye0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mitotic activity was significantly reduced in protoplasts derived from gibberellin‐treated tissues”
e1“Pisum sativum root cortical explants”
The same questions in other papers
class · yield · Lathyrus oleraceus (pea)Positive and negative results
5 papers in the same direction as this paper · 4 in the opposite direction
- no effect
- negative
- no effect
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.