Abstract
Root formation in rice transformants overexpressing mutated EL5 (mEL5) was severely inhibited because of meristematic cell death. Cell death was caused by nitrogen sources, particularly nitrate forms, in the culture medium. Nitrite treatment increased the cytokinin contents in roots, but mEL5 contained more cytokinins than non-transformants. Transcriptome profiling showed overlaps between nitrite-responsive genes in non-transformants and genes with altered expression in untreated mEL5. These results indicate that impairment of EL5 function activates nitrogen signaling despite the absence of a nitrogen source. Physical interaction between the EL5 C-terminal region and a cytosolic glyceraldehyde-3-phosphate dehydrogenase, OsGapC2, was demonstrated in vitro and in vivo. Elucidation of the role of glyceraldehyde-3-phosphate dehydrogenase in oxidative cell death in plants is expected in future.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
Root formation in rice transformants overexpressing mEL5 was severely inhibited.
- Taxon
- rice
Oryza sativae1 - Material
- transformantse1
- Treatment
- overexpressing mutated EL5 (mEL5)e1
- Gene
- EL5e1
- Outcome
- Root formatione1
- Direction
- severely inhibitede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Root formation in rice transformants overexpressing mutated EL5 (mEL5) was severely inhibited because of meristematic cell death”
Claim · model-written summary
Nitrogen sources, particularly nitrate forms, caused cell death in the culture medium in rice.
- Taxon
- riceOryza sativae1
- Organ / stage
- meristematice1
- Treatment
- nitrogen sources, particularly nitrate formse2
- Outcome
- Cell deathe2
- Setting
- culture mediume2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Root formation in rice transformants overexpressing mutated EL5 (mEL5) was severely inhibited because of meristematic cell death”
e2“Cell death was caused by nitrogen sources, particularly nitrate forms, in the culture medium”
Claim · model-written summary
Nitrite treatment increased cytokinin contents in rice roots.
- Taxon
- riceOryza sativae1
- Organ / stage
- rootse3
- Treatment
- Nitrite treatmente3
- Compound
- cytokinine3
- Outcome
- cytokinin contentse3
- Direction
- increasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Root formation in rice transformants overexpressing mutated EL5 (mEL5) was severely inhibited because of meristematic cell death”
e3“Nitrite treatment increased the cytokinin contents in roots”
Claim · model-written summary
mEL5 rice contained more cytokinins than non-transformants.
- Taxon
- riceOryza sativae1
- Material
- mEL5e4
- Compound
- cytokininse4
- Outcome
- cytokininse4
- Direction
- moree4
- Comparator
- non-transformantse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Root formation in rice transformants overexpressing mutated EL5 (mEL5) was severely inhibited because of meristematic cell death”
e4“mEL5 contained more cytokinins than non-transformants”
Claim · model-written summary
Nitrite-responsive genes in non-transformants overlapped with genes with altered expression in untreated mEL5 rice.
- Taxon
- riceOryza sativae1
- Material
- non-transformantse5
- Gene
- nitrite-responsive genese5
- Outcome
- overlaps between nitrite-responsive genese5
- Comparator
- untreated mEL5e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Root formation in rice transformants overexpressing mutated EL5 (mEL5) was severely inhibited because of meristematic cell death”
e5“Transcriptome profiling showed overlaps between nitrite-responsive genes in non-transformants and genes with altered expression in untreated mEL5”
Claim · model-written summary
Impairment of EL5 function is interpreted to activate nitrogen signalling in rice even without a nitrogen source.
- Taxon
- riceOryza sativae1
- Gene
- EL5e6
- Outcome
- nitrogen signalinge6
- Qualifier
- despite the absence of a nitrogen sourcee6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Root formation in rice transformants overexpressing mutated EL5 (mEL5) was severely inhibited because of meristematic cell death”
e6“These results indicate that impairment of EL5 function activates nitrogen signaling despite the absence of a nitrogen source”
Claim · model-written summary
The EL5 C-terminal region physically interacts with OsGapC2 in vitro and in vivo.
- Taxon
- riceOryza sativae1
- Gene
- OsGapC2e7
- Outcome
- Physical interactione7
- Qualifier
- in vitro and in vivoe7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Root formation in rice transformants overexpressing mutated EL5 (mEL5) was severely inhibited because of meristematic cell death”
e7“Physical interaction between the EL5 C-terminal region and a cytosolic glyceraldehyde-3-phosphate dehydrogenase, OsGapC2, was demonstrated in vitro and in vivo”
The same questions in other papers
nitrogen · cytology · Oryza sativa (rice)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.