Abstract
$Potato$ $virus$ $X$ (PVX) replication is precisely regulated by regulatory viral sequences and by viral and/or host proteins. In a previous study, we identified a 54-kDa cellular tobacco protein that bound to a region within the first 46 nucleotides (nt) of the 5' non-translated region (NTR) of the viral genome. Optimal binding was dependent upon the presence of an ACCA sequence at nt 10-13. To identify host factors that bind to 5' NTR elements including AC-rich sequences as well as stemloop 1 (SL1), we used northwestern blotting and matrixassisted laser desorption/ionization time-of-flight mass spectrometry for peptide mass fingerprinting. We screened several host factors that might affect PVX replication and selected a candidate protein, $Nicotiana$ $tabacum$ WRKY transcription factor 1 (NtWRKY1). We used a $Tobacco$ $rattle$ $virus$ (TRV)-based virus-induced gene silencing (VIGS) system to investigate the role of NtWRKY1 in PVX replication. Silencing of $WRKY1$ in $Nicotiana$ $benthamiana$ caused lethal apical necrosis and allowed an increase in PVX RNA accumulation. This result could reflect the balancing of PVX accumulation in a systemic $N.$ $benthamiana$ host to maintain PVX survival and still produce a suitable appearance of mosaic and mottle symptoms. Our results suggest that PVX may recruit the WRKY transcription factor, which binds to the 5' NTR of viral genomic RNA and acts as a key regulator of viral infection.
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Claims 4
Claim · model-written summary
NtWRKY1 was identified as a candidate host factor affecting PVX replication.
- Taxon
- $Nicotiana$ $tabacum$e0
- Interactor
- PVXPotato virus Xe1pathogen
- Gene
- NtWRKY1e0
- Outcome
- candidate proteine0
Not reportedDirection
Verbatim
e0“selected a candidate protein, $Nicotiana$ $tabacum$ WRKY transcription factor 1 (NtWRKY1)”
e1“host factors that might affect PVX replication”
Claim · model-written summary
Silencing WRKY1 caused lethal apical necrosis in Nicotiana benthamiana.
- Taxon
- $Nicotiana$ $benthamiana$e0
- Treatment
- Silencing of $WRKY1$e0
- Interactor
- PVXPotato virus Xe0pathogen
- Gene
- $WRKY1$e0
- Outcome
- lethal apical necrosise0
Not reportedComparatorDirection
Verbatim
e0“Silencing of $WRKY1$ in $Nicotiana$ $benthamiana$ caused lethal apical necrosis and allowed an increase in PVX RNA accumulation”
Claim · model-written summary
Silencing WRKY1 increased PVX RNA accumulation in Nicotiana benthamiana.
- Taxon
- $Nicotiana$ $benthamiana$e0
- Treatment
- Silencing of $WRKY1$e0
- Interactor
- PVXPotato virus Xe0pathogen
- Gene
- $WRKY1$e0
- Outcome
- PVX RNA accumulatione0
- Direction
- increasee0
Not reportedComparator
Verbatim
e0“Silencing of $WRKY1$ in $Nicotiana$ $benthamiana$ caused lethal apical necrosis and allowed an increase in PVX RNA accumulation”
Claim · model-written summary
The authors suggest PVX recruits WRKY transcription factor binding viral genomic RNA to regulate infection.
- Taxon
- Potato
Solanum tuberosumt0 - Interactor
- PVXPotato virus Xe0pathogen
- Gene
- WRKY transcription factore0
- Outcome
- viral infectione0
- Qualifier
- binds to the 5' NTR of viral genomic RNAe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PVX may recruit the WRKY transcription factor, which binds to the 5' NTR of viral genomic RNA and acts as a key regulator of viral infection”
t0“Potato virus X”
The same questions in other papers
loss of function · Potato virus X · Nicotiana benthamianaPositive and negative results
6 papers in the same direction as this paper · 8 in the opposite direction
- positive
- positive
- positive
11 papers more in this group
See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.