Abstract
Mugineic acid family phytosiderophores (MAs) are metal chelators that are produced in graminaceous plants in response to iron (Fe) deficiency, but current evidence regarding secretion of MAs during zinc (Zn) deficiency is contradictory. Our studies using HPLC analysis showed that Zn deficiency induces the synthesis and secretion of MAs in barley plants. The levels of the HvNAS1, HvNAAT-A, HvNAAT-B, HvIDS2 and HvIDS3 transcripts, which encode the enzymes involved in the synthesis of MAs, were increased in Zn-deficient roots. Studies of the genes involved in the methionine cycle using microarray analysis showed that the transcripts of these genes were increased in both Zn-deficient and Fe-deficient barley roots, probably allowing the plant to meet its demand for methionine, a precursor in the synthesis of MAs. In addition, HvNAAT-B transcripts were detected in Zn-deficient shoots, but not in those that were deficient in Fe. Increased synthesis of MAs in Zn-deficient barley was not due to a deficiency of Fe, because Zn-deficient barley accumulated more Fe than did the control plants, ferritin transcripts were increased in Zn-deficient plants, and Zn deficiency promoted Fe transport from root to shoot. Moreover, analysis using the positron-emitting tracer imaging system (PETIS) confirmed that more 62Zn(II)-MAs than 62Zn2+ were absorbed by the roots of Zn-deficient barley plants. These data suggest that the increased biosynthesis and secretion of MAs arising from a shortage of Zn are not due to an induced Fe deficiency, and that secreted MAs are effective in absorbing Zn from the soil.
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Claims 8
Claim · model-written summary
Zinc deficiency induced synthesis and secretion of MAs in barley.
- Taxon
- barley plants
.jpg?width=60)
Hordeum vulgaree0 - Treatment
- Zn deficiencye0
- Outcome
- synthesis and secretion of MAse0
- Direction
- inducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Zn deficiency induces the synthesis and secretion of MAs in barley plants”
Claim · model-written summary
HvNAS1, HvNAAT-A, HvNAAT-B, HvIDS2 and HvIDS3 transcript levels increased in zinc-deficient roots.
- Taxon
- barleyHordeum vulgaret0
- Organ / stage
- rootse0
- Treatment
- Zn-deficiente0
- Gene
- HvNAS1, HvNAAT-A, HvNAAT-B, HvIDS2 and HvIDS3e0
- Outcome
- transcriptse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The levels of the HvNAS1, HvNAAT-A, HvNAAT-B, HvIDS2 and HvIDS3 transcripts, which encode the enzymes involved in the synthesis of MAs, were increased in Zn-deficient roots.”
t0“barley”
Claim · model-written summary
Methionine-cycle transcripts increased in zinc-deficient and iron-deficient barley roots.
- Taxon
- barleyHordeum vulgaret0
- Organ / stage
- rootse0
- Treatment
- Zn-deficient and Fe-deficiente0
- Outcome
- transcripts of these genese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transcripts of these genes were increased in both Zn-deficient and Fe-deficient barley roots”
t0“barley”
Claim · model-written summary
HvNAAT-B transcripts were detected in zinc-deficient shoots but not iron-deficient shoots.
- Taxon
- barleyHordeum vulgaret0
- Organ / stage
- shootse0
- Gene
- HvNAAT-Be0
- Outcome
- HvNAAT-B transcripts were detected in Zn-deficient shoots, but not in those that were deficient in Fee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HvNAAT-B transcripts were detected in Zn-deficient shoots, but not in those that were deficient in Fe”
t0“barley”
Claim · model-written summary
Zinc-deficient barley accumulated more iron than control plants.
- Taxon
- barleyHordeum vulgaree0
- Treatment
- Zn-deficiente0
- Outcome
- Fee0
- Direction
- moree0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Zn-deficient barley accumulated more Fe than did the control plants”
Claim · model-written summary
Ferritin transcripts increased in zinc-deficient plants.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- Zn-deficiente0
- Gene
- ferritine0
- Outcome
- ferritin transcriptse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ferritin transcripts were increased in Zn-deficient plants”
t0“barley”
Claim · model-written summary
Zinc deficiency promoted iron transport from root to shoot.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- Zn deficiencye0
- Outcome
- Fe transport from root to shoote0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Zn deficiency promoted Fe transport from root to shoot”
t0“barley”
Claim · model-written summary
Zinc-deficient barley roots absorbed more 62Zn(II)-MAs than 62Zn2+.
- Taxon
- barley plantsHordeum vulgaree0
- Organ / stage
- rootse0
- Outcome
- 62Zn(II)-MAse0
- Direction
- moree0
- Comparator
- 62Zn2+e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“more 62Zn(II)-MAs than 62Zn2+ were absorbed by the roots of Zn-deficient barley plants”
The same questions in other papers
zinc atom · molecular: metabolites · Hordeum vulgare (barley)Positive and negative results
3 papers in the same direction as this paper · 5 in the opposite direction
This paper is about lacking the nutrient, so it counts the other way round here.
- positive
- positive
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.