Abstract
The Asparagus officinalis L. asparagine (Asn) synthetase (AS) promoter was analysed for elements responding to carbohydrate and senescence signals. Transgenic Arabidopsis thaliana L. plants containing deletion constructs of the -1958 bp AS promoter linked to the β-glucuronidase (GUS) reporter gene (AS::GUS) were analysed by measuring GUS specific activity. Inclusion of sucrose (Suc), glucose (Glc) or fructose (Fru) in plant media repressed levels of GUS activity in -1958AS::GUS plants, regardless of the light environment, with increases in GUS found 1 d after incubation on Suc-lacking media. Hexokinase is likely to be involved in the signal pathway, as Suc, Glc, Fru, 2-deoxy-d-glucose and mannose were more effective repressors than 3-O-methylglucose, and the hexokinase inhibitor mannoheptulose reduced repression. Plants containing AS::GUS constructs with deletions that reduced the promoter to less than -405 bp did not show low sugar induction. AS::GUS activity was significantly higher in excised leaves induced to senesce by dark storage for 24 h, compared to fresh leaves, for lines containing at least -640 bp of the AS promoter but not those with -523 bp or smaller promoter fragments. Fusion of the -640 to -523 bp region to a -381AS::GUS construct generated a promoter that retained senescence induction but lacked low sugar induction. Alignment of this region to the 33-bp senescence-related sequence of the Arabidopsis and Brassica napus L. SAG12 promoters identified the sequence TTGCACG as being conserved in all the promoters, and which may be an important senescence-responsive element.
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Claims 9
Claim · model-written summary
Sucrose, glucose and fructose repressed GUS activity independently of light.
- Taxon
- Arabidopsis thaliana L.
Arabidopsis thalianae1 - Material
- -1958AS::GUSe0
- Treatment
- sucrose (Suc), glucose (Glc) or fructose (Fru)e0
- Outcome
- GUS activitye0
- Direction
- repressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inclusion of sucrose (Suc), glucose (Glc) or fructose (Fru) in plant media repressed levels of GUS activity in -1958AS::GUS plants, regardless of the light environment”
e1“Transgenic Arabidopsis thaliana L. plants containing deletion constructs of the -1958 bp AS promoter linked to the β-glucuronidase (GUS) reporter gene (AS::GUS) were analysed by measuring GUS specific activity.”
t0“Asparagus officinalis”
Claim · model-written summary
GUS activity increased one day after transfer to sucrose-free medium.
- Taxon
- Arabidopsis thaliana L.Arabidopsis thalianae1
- Treatment
- Suc-lacking mediae0
- Outcome
- GUSe0
- Direction
- increasese0
- Qualifier
- 1 de0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increases in GUS found 1 d after incubation on Suc-lacking media.”
e1“Transgenic Arabidopsis thaliana L. plants containing deletion constructs of the -1958 bp AS promoter linked to the β-glucuronidase (GUS) reporter gene (AS::GUS) were analysed by measuring GUS specific activity.”
t0“Asparagus officinalis”
Claim · model-written summary
Sucrose, glucose, fructose, 2-deoxy-d-glucose and mannose repressed more effectively than 3-O-methylglucose.
- Taxon
- Arabidopsis thaliana L.Arabidopsis thalianae1
- Treatment
- Suc, Glc, Fru, 2-deoxy-d-glucose and mannosee0
- Outcome
- repressorse0
- Direction
- more effectivee0
- Comparator
- 3-O-methylglucosee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Suc, Glc, Fru, 2-deoxy-d-glucose and mannose were more effective repressors than 3-O-methylglucose”
e1“Transgenic Arabidopsis thaliana L. plants containing deletion constructs of the -1958 bp AS promoter linked to the β-glucuronidase (GUS) reporter gene (AS::GUS) were analysed by measuring GUS specific activity.”
t0“Asparagus officinalis”
Claim · model-written summary
Mannoheptulose reduced sugar repression.
- Taxon
- Arabidopsis thaliana L.Arabidopsis thalianae1
- Treatment
- mannoheptulosee0
- Outcome
- repressione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the hexokinase inhibitor mannoheptulose reduced repression.”
e1“Transgenic Arabidopsis thaliana L. plants containing deletion constructs of the -1958 bp AS promoter linked to the β-glucuronidase (GUS) reporter gene (AS::GUS) were analysed by measuring GUS specific activity.”
t0“Asparagus officinalis”
Claim · model-written summary
Promoter deletions below -405 bp abolished low sugar induction.
- Taxon
- Arabidopsis thaliana L.Arabidopsis thalianae1
- Treatment
- deletions that reduced the promoter to less than -405 bpe0
- Gene
- AS::GUSe0
- Outcome
- low sugar inductione0
- Direction
- did not showe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plants containing AS::GUS constructs with deletions that reduced the promoter to less than -405 bp did not show low sugar induction.”
e1“Transgenic Arabidopsis thaliana L. plants containing deletion constructs of the -1958 bp AS promoter linked to the β-glucuronidase (GUS) reporter gene (AS::GUS) were analysed by measuring GUS specific activity.”
t0“Asparagus officinalis”
Claim · model-written summary
Dark storage raised GUS activity in senescing excised leaves with at least -640 bp promoter.
- Taxon
- Arabidopsis thaliana L.Arabidopsis thalianae1
- Organ / stage
- excised leavese0
- Treatment
- dark storage for 24 he0
- Outcome
- AS::GUS activitye0
- Direction
- highere0
- Qualifier
- at least -640 bpe0
- Comparator
- fresh leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AS::GUS activity was significantly higher in excised leaves induced to senesce by dark storage for 24 h, compared to fresh leaves, for lines containing at least -640 bp of the AS promoter but not those with -523 bp or smaller promoter fragments.”
e1“Transgenic Arabidopsis thaliana L. plants containing deletion constructs of the -1958 bp AS promoter linked to the β-glucuronidase (GUS) reporter gene (AS::GUS) were analysed by measuring GUS specific activity.”
t0“Asparagus officinalis”
Claim · model-written summary
The -640 to -523 bp promoter fusion retained senescence induction.
- Taxon
- Arabidopsis thaliana L.Arabidopsis thalianae1
- Gene
- -381AS::GUSe0
- Outcome
- retained senescence inductione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fusion of the -640 to -523 bp region to a -381AS::GUS construct generated a promoter that retained senescence induction but lacked low sugar induction.”
e1“Transgenic Arabidopsis thaliana L. plants containing deletion constructs of the -1958 bp AS promoter linked to the β-glucuronidase (GUS) reporter gene (AS::GUS) were analysed by measuring GUS specific activity.”
t0“Asparagus officinalis”
Claim · model-written summary
The -640 to -523 bp promoter fusion lacked low sugar induction.
- Taxon
- Arabidopsis thaliana L.Arabidopsis thalianae1
- Gene
- -381AS::GUSe0
- Outcome
- lacked low sugar inductione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fusion of the -640 to -523 bp region to a -381AS::GUS construct generated a promoter that retained senescence induction but lacked low sugar induction.”
e1“Transgenic Arabidopsis thaliana L. plants containing deletion constructs of the -1958 bp AS promoter linked to the β-glucuronidase (GUS) reporter gene (AS::GUS) were analysed by measuring GUS specific activity.”
t0“Asparagus officinalis”
Claim · model-written summary
TTGCACG was conserved across aligned senescence-related promoters.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- TTGCACGe0
- Outcome
- conserved in all the promoterse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Alignment of this region to the 33-bp senescence-related sequence of the Arabidopsis and Brassica napus L. SAG12 promoters identified the sequence TTGCACG as being conserved in all the promoters, and which may be an important senescence-responsive element.”
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See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.