Abstract
Solutions were obtained from the cell wall free space of red light-grown cucumber (Cucumis sativus L.) hypocotyl sections by a low-speed centrifugation technique. The centrifugate contained NAD and peroxidase but no detectable cytoplasmic contamination, as indicated by the absence of the activity of glucose-6-phosphate dehydrogenase from the cell wall solution. Peroxidase activity centrifuged from the cell wall of red light-grown cucumber hypocotyl section could be resolved into at least three cathodic isoforms and two anodic isoforms by isoelectric focusing. Treatment of red light-grown cucumber seedlings with a 10-minute pulse of high-intensity blue light increased the level of cell wall peroxidase by about 60% and caused a qualitative change in the anodic isoforms of this enzyme. The increase in peroxidase activity was detectable within 25 minutes after the start of the blue light pulse, was maximal at 35 minutes, and declined to control levels by 45 minutes of irradiation. The inhibitory effect of blue light on hypocotyl elongation was more rapid than the effect of blue light on total wall peroxidase activity, leading to the conclusion that growth and peroxidase activity are not causally related.
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Claims 9
Claim · model-written summary
Cucumber hypocotyl cell wall solution contained NAD.
Not reportedTaxon
Verbatim
e0“The centrifugate contained NAD and peroxidase but no detectable cytoplasmic contamination, as indicated by the absence of the activity of glucose-6-phosphate dehydrogenase from the cell wall solution.”
t0“Cucumis Hypocotyls”
Claim · model-written summary
Cucumber hypocotyl cell wall solution contained peroxidase.
Not reportedTaxon
Verbatim
e0“The centrifugate contained NAD and peroxidase but no detectable cytoplasmic contamination, as indicated by the absence of the activity of glucose-6-phosphate dehydrogenase from the cell wall solution.”
t0“Cucumis Hypocotyls”
Claim · model-written summary
Cucumber hypocotyl cell wall solution contained no detectable cytoplasmic contamination.
Not reportedTaxon
Verbatim
e0“The centrifugate contained NAD and peroxidase but no detectable cytoplasmic contamination, as indicated by the absence of the activity of glucose-6-phosphate dehydrogenase from the cell wall solution.”
t0“Cucumis Hypocotyls”
Claim · model-written summary
Cucumber hypocotyl wall peroxidase resolved into at least three cathodic and two anodic isoforms.
- Taxon
- cucumber
Cucumis sativuse0 - Organ / stage
- hypocotyl sectione0
- Gene
- Peroxidasee0
- Outcome
- at least three cathodic isoforms and two anodic isoformse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Peroxidase activity centrifuged from the cell wall of red light-grown cucumber hypocotyl section could be resolved into at least three cathodic isoforms and two anodic isoforms by isoelectric focusing.”
Claim · model-written summary
A ten minute blue light pulse increased cucumber cell wall peroxidase about 60%.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- a 10-minute pulse of high-intensity blue lighte0
- Gene
- peroxidasee0
- Outcome
- level of cell wall peroxidasee0
- Direction
- increasede0
- Value
- about 60%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment of red light-grown cucumber seedlings with a 10-minute pulse of high-intensity blue light increased the level of cell wall peroxidase by about 60% and caused a qualitative change in the anodic isoforms of this enzyme.”
Claim · model-written summary
Blue light changed the anodic peroxidase isoforms in cucumber seedlings.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- a 10-minute pulse of high-intensity blue lighte0
- Gene
- peroxidasee0
- Outcome
- qualitative change in the anodic isoformse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment of red light-grown cucumber seedlings with a 10-minute pulse of high-intensity blue light increased the level of cell wall peroxidase by about 60% and caused a qualitative change in the anodic isoforms of this enzyme.”
Claim · model-written summary
Cucumber wall peroxidase activity rose within 25 minutes, peaked at 35 minutes and returned to control levels by 45 minutes.
- Outcome
- peroxidase activitye0
- Direction
- increasee0
- Qualifier
- within 25 minutes after the start of the blue light pulse, was maximal at 35 minutes, and declined to control levels by 45 minutes of irradiatione0
Not reportedTaxon
Verbatim
e0“The increase in peroxidase activity was detectable within 25 minutes after the start of the blue light pulse, was maximal at 35 minutes, and declined to control levels by 45 minutes of irradiation.”
t0“Cucumis Hypocotyls”
Claim · model-written summary
Blue light inhibited cucumber hypocotyl elongation more rapidly than it altered wall peroxidase activity.
Not reportedTaxonComparator
Verbatim
e0“The inhibitory effect of blue light on hypocotyl elongation was more rapid than the effect of blue light on total wall peroxidase activity, leading to the conclusion that growth and peroxidase activity are not causally related.”
t0“Cucumis Hypocotyls”
Claim · model-written summary
The authors concluded cucumber hypocotyl growth and wall peroxidase activity were not causally related.
Not reportedTaxon
Verbatim
e0“The inhibitory effect of blue light on hypocotyl elongation was more rapid than the effect of blue light on total wall peroxidase activity, leading to the conclusion that growth and peroxidase activity are not causally related.”
t0“Cucumis Hypocotyls”
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See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.