Abstract
Abstract The constituents of cottonseed pigment glands were fractionated by the use of column chromatography with DEAE cellulose ion exchanger and silicic acid, and a new green pigment was isolated. The acute oral toxicity of the new pigment was determined using rats as experimental animals. The LD‐50 value obtained was 0.66 g/kg of body weight indicating that the new pigment which was named gossyverdurin is the most toxic of any cottonseed pigment so far reported. Gossyverdurin showed absorption maxima at 250, 370, and 560 mॖ. Reaction with para‐anisidine under the conditions used for the determination of gossypol gave an absorption peak similar to that obtained with gossypol indicating that the new compound is structurally related to gossypol. In addition a second peak at 342 mॖ appears on reaction with para‐anisidine indicating important structural differences between gossypol and gossyverdurin.
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Claims 6
Claim · model-written summary
A new green pigment was isolated from cottonseed pigment glands.
Verbatim
t0“cottonseed pigment glands”
e0“a new green pigment was isolated.”
e1“the new pigment which was named gossyverdurin”
Claim · model-written summary
The pigment was named gossyverdurin.
Verbatim
t0“cottonseed pigment glands”
e1“the new pigment which was named gossyverdurin”
Claim · model-written summary
Gossyverdurin had absorption maxima at 250, 370 and 560 mॖ.
Verbatim
t0“cottonseed pigment glands”
e2“Gossyverdurin showed absorption maxima at 250, 370, and 560 mॖ.”
Claim · model-written summary
The para‐anisidine absorption response indicated structural relation to gossypol.
Verbatim
t0“cottonseed pigment glands”
e3“Reaction with para‐anisidine under the conditions used for the determination of gossypol gave an absorption peak similar to that obtained with gossypol indicating that the new compound is structurally related to gossypol.”
Claim · model-written summary
A second 342 mॖ peak with para‐anisidine indicated structural differences from gossypol.
Verbatim
t0“cottonseed pigment glands”
e4“a second peak at 342 mॖ appears on reaction with para‐anisidine indicating important structural differences between gossypol and gossyverdurin.”
Claim · model-written summary
Gossyverdurin was considered the most toxic reported cottonseed pigment.
Verbatim
t0“cottonseed pigment glands”
e5“the most toxic of any cottonseed pigment so far reported”