Abstract
The antagonistic interaction between iron (Fe) and phosphorus (P) has been noted in the area of plant nutrition. To understand the physiology and molecular mechanisms of this interaction, we studied the growth performance, nutrient concentration, and gene expression profiles of root and shoot segments derived from 10-d-old rice (Oryza sativa) seedlings under four different nutrient conditions: (1) full strength of Fe and P (+Fe+P); (2) full strength of P and no Fe (-Fe+P); (3) full strength of Fe and no P (+Fe-P); and (4) without both Fe and P (-Fe-P). While removal of Fe in the growth medium resulted in very low shoot and root Fe concentrations, the chlorotic symptoms and retarded seedling growth were only observed on seedlings grown in the presence of P. Microarray data showed that in roots, 7,628 transcripts were significantly changed in abundance in the absence of Fe alone. Interestingly, many of these changes were reversed if P was also absent (-Fe-P), with only approximately 15% overlapping with -Fe alone (-Fe+P). Analysis of the soluble Fe concentration in rice seedling shoots showed that P deficiency resulted in significantly increased Fe availability within the plants. The soluble Fe concentration under -Fe-P conditions was similar to that under +Fe+P conditions. These results provide evidence that the presence of P can affect Fe availability and in turn can influence the regulation of Fe-responsive genes.
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Claims 8
Claim · model-written summary
Removing iron led to very low shoot and root iron concentration.
- Taxon
- rice (Oryza sativa)
Oryza sativae1 - Treatment
- removal of Fee0
- Outcome
- shoot and root Fe concentrationse0
- Direction
- very lowe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“removal of Fe in the growth medium resulted in very low shoot and root Fe concentrations”
e1“rice (Oryza sativa) seedlings”
Claim · model-written summary
Chlorosis and retarded growth after iron removal appeared only when phosphorus was present.
- Taxon
- rice (Oryza sativa)Oryza sativae1
- Outcome
- chlorotic symptoms and retarded seedling growthe0
- Qualifier
- presence of Pe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the chlorotic symptoms and retarded seedling growth were only observed on seedlings grown in the presence of P.”
e1“rice (Oryza sativa) seedlings”
Claim · model-written summary
Iron deficiency alone significantly changed abundance of 7,628 root transcripts.
- Taxon
- rice (Oryza sativa)Oryza sativae1
- Organ / stage
- rootse0
- Treatment
- absence of Fe alonee0
- Outcome
- transcripts were significantly changed in abundancee0
- Value
- 7,628 transcriptse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in roots, 7,628 transcripts were significantly changed in abundance in the absence of Fe alone.”
e1“rice (Oryza sativa) seedlings”
Claim · model-written summary
Many iron-responsive transcript changes reversed with concurrent phosphorus absence.
- Taxon
- rice (Oryza sativa)Oryza sativae1
- Treatment
- P was also absent (-Fe-P)e0
- Outcome
- these changese0
- Direction
- reversede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“many of these changes were reversed if P was also absent (-Fe-P)”
e1“rice (Oryza sativa) seedlings”
Claim · model-written summary
Only approximately 15% of transcript changes overlapped between combined deficiency and iron deficiency alone.
- Taxon
- rice (Oryza sativa)Oryza sativae1
- Outcome
- overlappinge0
- Value
- approximately 15%e0
- Comparator
- -Fe alone (-Fe+P)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only approximately 15% overlapping with -Fe alone (-Fe+P).”
e1“rice (Oryza sativa) seedlings”
Claim · model-written summary
Phosphorus deficiency significantly increased soluble iron availability in seedlings.
- Taxon
- rice (Oryza sativa)Oryza sativae1
- Treatment
- P deficiencye0
- Outcome
- Fe availabilitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“P deficiency resulted in significantly increased Fe availability within the plants.”
e1“rice (Oryza sativa) seedlings”
Claim · model-written summary
Soluble iron under combined deficiency was similar to full iron and phosphorus supply.
- Taxon
- rice (Oryza sativa)Oryza sativae1
- Outcome
- soluble Fe concentratione0
- Direction
- similare0
- Comparator
- +Fe+P conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The soluble Fe concentration under -Fe-P conditions was similar to that under +Fe+P conditions.”
e1“rice (Oryza sativa) seedlings”
Claim · model-written summary
Authors infer that phosphorus presence affects iron availability and regulation of iron-responsive genes.
- Taxon
- rice (Oryza sativa)Oryza sativae1
- Gene
- Fe-responsive genese0
- Compound
- Pe0
- Outcome
- Fe availabilitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the presence of P can affect Fe availability and in turn can influence the regulation of Fe-responsive genes.”
e1“rice (Oryza sativa) seedlings”
The same questions in other papers
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This paper is about lacking the nutrient, so it counts the other way round here.
- positive
- positive
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See all 168 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.