Abstract
The sequence of nitrate reductase (EC 1.6.6.1) mRNA from the plant Arabidopsis thaliana has been determined. A 3.0-kilobase-long cDNA was isolated from a lambda gt10 cDNA library of Arabidopsis leaf poly(A)+ RNA. The cDNA hybridized to a 3.2-kilobase mRNA whose level increased 15-fold in response to treatment of the plant with nitrate. An open reading frame encoding a 917 amino acid protein was found in the sequence. This protein is very similar to tobacco nitrate reductase, being greater than 80% identical within a section of 450 amino acids. By comparing the Arabidopsis protein sequence with other protein sequences, three functional domains were deduced: (i) a molybdenum-pterin-binding domain that is similar to the molybdenum-pterin-binding domain of rat liver sulfite oxidase, (ii) a heme-binding domain that is similar to proteins in the cytochrome b5 superfamily, and (iii) an FAD-binding domain that is similar to NADH-cytochrome b5 reductase.
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Claims 5
Claim · model-written summary
The Arabidopsis nitrate reductase mRNA sequence was determined and a 3.0-kilobase cDNA isolated.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Organ / stage
- leafe0
- Gene
- nitrate reductasee0
- Outcome
- cDNAe0
- Value
- 3.0-kilobase-longe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The sequence of nitrate reductase (EC 1.6.6.1) mRNA from the plant Arabidopsis thaliana has been determined. A 3.0-kilobase-long cDNA was isolated from a lambda gt10 cDNA library of Arabidopsis leaf poly(A)+ RNA.”
Claim · model-written summary
Nitrate treatment increased the level of the 3.2-kilobase nitrate reductase mRNA 15-fold.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- treatment of the plant with nitratee0
- Outcome
- mRNA whose levele0
- Direction
- increasede0
- Value
- 15-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cDNA hybridized to a 3.2-kilobase mRNA whose level increased 15-fold in response to treatment of the plant with nitrate.”
t0“Arabidopsis thaliana”
Claim · model-written summary
The nitrate reductase sequence contained an open reading frame encoding 917 amino acids.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- open reading framee0
- Value
- 917 amino acide0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An open reading frame encoding a 917 amino acid protein was found in the sequence.”
t0“Arabidopsis thaliana”
Claim · model-written summary
Arabidopsis nitrate reductase was greater than 80% identical to tobacco nitrate reductase over 450 amino acids.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- nitrate reductaset0
- Outcome
- identicale0
- Value
- greater than 80%e0
- Qualifier
- 450 amino acidse0
- Comparator
- tobacco nitrate reductasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This protein is very similar to tobacco nitrate reductase, being greater than 80% identical within a section of 450 amino acids.”
t0“Arabidopsis thaliana mRNA encoding nitrate reductase”
Claim · model-written summary
Sequence comparisons identified molybdenum-pterin, heme and FAD-binding domains in Arabidopsis nitrate reductase.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- three functional domainse0
- Value
- threee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“three functional domains were deduced: (i) a molybdenum-pterin-binding domain that is similar to the molybdenum-pterin-binding domain of rat liver sulfite oxidase, (ii) a heme-binding domain that is similar to proteins in the cytochrome b5 superfamily, and (iii) an FAD-binding domain that is similar to NADH-cytochrome b5 reductase.”
t0“Arabidopsis thaliana”
The same questions in other papers
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- no effect
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See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.