Abstract
Arabidopsis transcription factors, MYB28, MYB29 and MYB76, positively regulate aliphatic glucosinolate (AGSL) biosynthesis. Mutual transcriptional regulation among these MYB genes makes it difficult to elucidate their individual function simply by analyzing knock-out mutants or ectopically overexpressing lines of these genes. In this study, we constructed transgenic lines expressing each MYB gene driven by its own promoter in the myb28myb29 background, where the expression of the endogenous MYB28, MYB29 and MYB76 was repressed with no AGSL accumulation. In leaves, transgenic MYB28 expression activated AGSL biosynthetic genes and restored accumulation of AGSLs with short side chains. Transgenic MYB29 expression activated the same biosynthetic pathway, but induction of the genes involved in side chain elongation was weaker than that by MYB28, resulting in a weaker recovery of AGSLs. Neither MYB28 nor MYB29 recovered long-chain AGSL accumulation. MYB76 was considered to require both MYB28 and MYB29 for its normal level of expression in leaves, and could not activate AGSL biosynthesis on its own. Interestingly, the accumulation in seeds of long- and short-chain AGSLs was restored by transgenic expression of MYB28 and MYB76, respectively. A sulfur stress experiment indicated that MYB28 expression was induced by sulfur deficiency, while the expression levels of MYB29 and MYB76 were positively correlated with sulfur concentration. This study illustrated how the individual MYBs work in regulating AGSL biosynthesis when expressed alone under normal transcriptional regulation.
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Claims 11
Claim · model-written summary
MYB28 expression activated biosynthetic genes and restored short-chain AGSL accumulation in leaves.
- Taxon
- Arabidopsis
Arabidopsist0 - Organ / stage
- leavese0
- Treatment
- transgenic MYB28 expressione0
- Gene
- MYB28e0
- Outcome
- AGSL biosynthetic genes and restored accumulation of AGSLs with short side chainse0
- Direction
- restorede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In leaves, transgenic MYB28 expression activated AGSL biosynthetic genes and restored accumulation of AGSLs with short side chains”
t0“Arabidopsis”
Claim · model-written summary
MYB29 activated the same pathway but induced side chain elongation genes less strongly than MYB28.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Transgenic MYB29 expressione0
- Gene
- MYB29e0
- Outcome
- induction of the genes involved in side chain elongatione0
- Direction
- weakere0
- Comparator
- MYB28e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic MYB29 expression activated the same biosynthetic pathway, but induction of the genes involved in side chain elongation was weaker than that by MYB28”
t0“Arabidopsis”
Claim · model-written summary
MYB29 gave weaker AGSL recovery than MYB28.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Transgenic MYB29 expressione2
- Gene
- MYB29e2
- Outcome
- recovery of AGSLse0
- Direction
- weakere0
- Comparator
- MYB28e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“resulting in a weaker recovery of AGSLs”
e1“than that by MYB28”
e2“Transgenic MYB29 expression”
t0“Arabidopsis”
Claim · model-written summary
Neither MYB28 nor MYB29 restored long-chain AGSL accumulation.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- MYB28e0
- Outcome
- long-chain AGSL accumulatione0
- Direction
- Neithere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Neither MYB28 nor MYB29 recovered long-chain AGSL accumulation”
t0“Arabidopsis”
Claim · model-written summary
MYB76 required MYB28 and MYB29 for normal leaf expression, according to the authors.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- leavese0
- Gene
- MYB76e0
- Outcome
- normal level of expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MYB76 was considered to require both MYB28 and MYB29 for its normal level of expression in leaves”
t0“Arabidopsis”
Claim · model-written summary
MYB76 alone did not activate AGSL biosynthesis.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- MYB76e0
- Gene
- MYB76e0
- Outcome
- AGSL biosynthesise0
- Direction
- could not activatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MYB76 was considered to require both MYB28 and MYB29 for its normal level of expression in leaves, and could not activate AGSL biosynthesis on its own”
t0“Arabidopsis”
Claim · model-written summary
MYB28 expression restored long-chain AGSL accumulation in seeds.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- seedse0
- Treatment
- transgenic expression of MYB28e0
- Gene
- MYB28e0
- Outcome
- long- and short-chain AGSLse0
- Direction
- restorede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the accumulation in seeds of long- and short-chain AGSLs was restored by transgenic expression of MYB28 and MYB76, respectively”
t0“Arabidopsis”
Claim · model-written summary
MYB76 expression restored short-chain AGSL accumulation in seeds.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- seedse0
- Treatment
- transgenic expression of MYB28 and MYB76e0
- Gene
- MYB76e0
- Outcome
- long- and short-chain AGSLse0
- Direction
- restorede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the accumulation in seeds of long- and short-chain AGSLs was restored by transgenic expression of MYB28 and MYB76, respectively”
t0“Arabidopsis”
Claim · model-written summary
Sulfur deficiency induced MYB28 expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- sulfur deficiencye0
- Gene
- MYB28e0
- Outcome
- MYB28 expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MYB28 expression was induced by sulfur deficiency”
t0“Arabidopsis”
Claim · model-written summary
MYB29 expression correlated positively with sulfur concentration.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- MYB29e0
- Compound
- sulfure0
- Outcome
- expression levels of MYB29e0
- Direction
- positively correlatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression levels of MYB29 and MYB76 were positively correlated with sulfur concentration”
t0“Arabidopsis”
Claim · model-written summary
MYB76 expression correlated positively with sulfur concentration.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- MYB76e0
- Compound
- sulfure0
- Outcome
- expression levels of MYB29 and MYB76e0
- Direction
- positively correlatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression levels of MYB29 and MYB76 were positively correlated with sulfur concentration”
t0“Arabidopsis”
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