Paper · 1977 · PLANT PHYSIOLOGY

Respiration and the Energy Requirement for Nitrogen Fixation in Nodulated Pea Roots

John D. Mahon

7
claims
15
verbatim quotes
62
citations
3
research areas
✓
Open access

Abstract

Pisum sativum L. cv. Trapper plants were inoculated and grown in a controlled environment on N-free nutrient solution. After 4 weeks N was supplied to treatment plants as NH(4)NO(3), KNO(3), or NH(4)Cl and rates of C(2)H(2) reduction, root + nodule respiration, and leaf photosynthesis were determined 1 week later. The increase in respiration per unit of C(2)H(2) reduction was not affected by either the form of N added or the light conditions during growth, although the basal respiration rate with no C(2)H(2) reduction increased with irradiance level. The mean regression coefficient from plots of respiration versus C(2)H(2) reduction was 0.23 + 0.04 (P [unk] .01) mg of CO(2) (mumol of C(2)H(2) reduced)(-1) which was very similar to the value for the coefficient of respiration associated with nitrogenase activity estimated by subtracting growth and maintenance respiration. Since the rate of N accumulation in N-free nutrient conditions was proportional to the rate of C(2)H(2) reduction, it appears that the method gives a true estimate of the energy requirements for N fixation which for these conditions was equivalent to 17 grams of carbohydrate consumed per gram of N fixed.

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Claims 7

Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

The respiration increase per unit of acetylene reduction was not affected by the form of N added.

Taxon
Pisum sativumLathyrus oleraceuse0
Treatment
the form of N addede1
Outcome
increase in respiration per unit of C(2)H(2) reductione1
Direction
not affectede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Pisum sativum L. cv. Trapper plants were inoculated and grown in a controlled environment on N-free nutrient solution”

✓ located verbatim in the source · abstract, characters 0–117 · show in text ↑
e1

“The increase in respiration per unit of C(2)H(2) reduction was not affected by either the form of N added or the light conditions during growth”

✓ located verbatim in the source · abstract, characters 316–459 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

The respiration increase per unit of acetylene reduction was not affected by light conditions during growth.

Taxon
Pisum sativumLathyrus oleraceuse0
Treatment
light conditions during growthe1
Outcome
increase in respiration per unit of C(2)H(2) reductione1
Direction
not affectede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Pisum sativum L. cv. Trapper plants were inoculated and grown in a controlled environment on N-free nutrient solution”

✓ located verbatim in the source · abstract, characters 0–117 · show in text ↑
e1

“The increase in respiration per unit of C(2)H(2) reduction was not affected by either the form of N added or the light conditions during growth”

✓ located verbatim in the source · abstract, characters 316–459 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Basal respiration without acetylene reduction increased with irradiance level.

Taxon
Pisum sativumLathyrus oleraceuse0
Treatment
irradiance levele2
Outcome
basal respiration rate with no C(2)H(2) reductione2
Direction
increasede2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Pisum sativum L. cv. Trapper plants were inoculated and grown in a controlled environment on N-free nutrient solution”

✓ located verbatim in the source · abstract, characters 0–117 · show in text ↑
e2

“the basal respiration rate with no C(2)H(2) reduction increased with irradiance level”

✓ located verbatim in the source · abstract, characters 470–555 · show in text ↑
Associationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Respiration increased with acetylene reduction, with a regression coefficient of 0.23 + 0.04 mg CO2 per umol C2H2 reduced.

Taxon
Pisum sativumLathyrus oleraceuse0
Material
cv. Trappere0
Outcome
respiration versus C(2)H(2) reductione3
Direction
increaseinferrede3
Value
0.23 + 0.04e3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Pisum sativum L. cv. Trapper plants were inoculated and grown in a controlled environment on N-free nutrient solution”

✓ located verbatim in the source · abstract, characters 0–117 · show in text ↑
e3

“The mean regression coefficient from plots of respiration versus C(2)H(2) reduction was 0.23 + 0.04 (P [unk] .01) mg of CO(2) (mumol of C(2)H(2) reduced)(-1)”

✓ located verbatim in the source · abstract, characters 557–714 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Rate of N accumulation was proportional to the rate of acetylene reduction under N-free nutrient conditions.

Taxon
Pisum sativumLathyrus oleraceuse0
Outcome
rate of N accumulatione4
Direction
increaseinferrede4
Setting
N-free nutrient conditionse4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Pisum sativum L. cv. Trapper plants were inoculated and grown in a controlled environment on N-free nutrient solution”

✓ located verbatim in the source · abstract, characters 0–117 · show in text ↑
e4

“Since the rate of N accumulation in N-free nutrient conditions was proportional to the rate of C(2)H(2) reduction”

✓ located verbatim in the source · abstract, characters 884–997 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

The acetylene reduction method gives a true estimate of the energy requirement for N fixation, 17 grams of carbohydrate per gram of N fixed under these conditions.

Taxon
Pisum sativumLathyrus oleraceuse0
Outcome
energy requirements for N fixatione5
Value
17 grams of carbohydrate consumed per gram of N fixede5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Pisum sativum L. cv. Trapper plants were inoculated and grown in a controlled environment on N-free nutrient solution”

✓ located verbatim in the source · abstract, characters 0–117 · show in text ↑
e5

“the method gives a true estimate of the energy requirements for N fixation which for these conditions was equivalent to 17 grams of carbohydrate consumed per gram of N fixed”

✓ located verbatim in the source · abstract, characters 1015–1188 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

The regression coefficient of 0.23 + 0.04 was very similar to the respiration coefficient of nitrogenase activity estimated by subtracting growth and maintenance respiration.

Taxon
Pisum sativumLathyrus oleraceuse0
Outcome
coefficient of respiration associated with nitrogenase activitye6
Value
0.23 + 0.04e3
Comparator
estimated by subtracting growth and maintenance respiratione6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Pisum sativum L. cv. Trapper plants were inoculated and grown in a controlled environment on N-free nutrient solution”

✓ located verbatim in the source · abstract, characters 0–117 · show in text ↑
e3

“The mean regression coefficient from plots of respiration versus C(2)H(2) reduction was 0.23 + 0.04 (P [unk] .01) mg of CO(2) (mumol of C(2)H(2) reduced)(-1)”

✓ located verbatim in the source · abstract, characters 557–714 · show in text ↑
e6

“which was very similar to the value for the coefficient of respiration associated with nitrogenase activity estimated by subtracting growth and maintenance respiration”

✓ located verbatim in the source · abstract, characters 715–882 · show in text ↑

light · physiology: respiration · Lathyrus oleraceus (pea)Positive and negative results

3 papers: 2 positive, 1 negative or no effect

2 in the opposite direction

  1. positive
  2. positive
See all 3 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.