Abstract
The environmental sensitivity of the processes associated with the import of photosynthate by developing soybean seeds was investigated within intact fruit and with excised, immature embryos. Intact pods of field-grown (Glycine max [L.] Merr.) Amsoy 71 soybeans were subjected to localized regimes of 0, 21, or 100% O(2) and 15, 25, or 35 degrees C during pulsechase translocation experiments and, 2.5 hours later, the uptake and distribution of (14)C-photosynthate among dissected fruit tissues determined. In other experiments, excised embryos were incubated in [(14)C]sucrose solutions under various experimental conditions to separate the effects of these treatments on accumulation by the embryos from those which may operate on phloem unloading in the maternal seedcoat.Import of (14)C-photosynthate by intact soybean fruit was both temperature- and O(2)-dependent. This dependency was shown to occur only within the seeds; import by the pod walls was essentially insensitive to fruit temperature or O(2) treatments. The embryos of anaerobic fruit were completely unlabeled, regardless of fruit temperature. But under anaerobic in vitro incubation conditions, uptake of [(14)C]sucrose in excised embryos was only 30% less than that in aerobic in vitro conditions. The data suggest that, within intact fruit, anoxia prevented sucrose efflux from the seed coat phloem and any subsequent uptake by the embryo. The demonstrated energy dependence of phloem unloading may reflect requirements for membrane integrity or energy metabolism in the companion cell-sieve element complex, consistent with a facilitated unloading process.Collectively, these data characterize the environmental sensitivity of photosynthate import in developing soybean fruit. They imply that environmental regulation of import may occur at both the embryo level and at the phloem terminals within the seed coat.
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Claims 8
Claim · model-written summary
Photosynthate import by intact soybean fruit depended on temperature and oxygen.
- Taxon
- soybean
Glycine maxe0 - Treatment
- temperature- and O(2)-dependente0
- Outcome
- Import of (14)C-photosynthate by intact soybean fruite0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Import of (14)C-photosynthate by intact soybean fruit was both temperature- and O(2)-dependent.”
Claim · model-written summary
Photosynthate import into soybean seeds depended on temperature and oxygen.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- seedse0
- Treatment
- fruit temperature or O(2) treatmentse0
- Outcome
- dependencye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This dependency was shown to occur only within the seeds; import by the pod walls was essentially insensitive to fruit temperature or O(2) treatments.”
t0“Developing Soybean Seeds”
Claim · model-written summary
Photosynthate import into soybean pod walls was essentially insensitive to fruit temperature or oxygen treatments.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- pod wallse0
- Treatment
- fruit temperature or O(2) treatmentse0
- Outcome
- import by the pod wallse0
- Direction
- essentially insensitivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“import by the pod walls was essentially insensitive to fruit temperature or O(2) treatments.”
t0“Developing Soybean Seeds”
Claim · model-written summary
Embryos in anaerobic soybean fruit were completely unlabeled regardless of fruit temperature.
- Taxon
- soybeanGlycine maxt0
- Treatment
- anaerobic fruite0
- Outcome
- embryos of anaerobic fruit were completely unlabelede0
- Qualifier
- regardless of fruit temperaturee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The embryos of anaerobic fruit were completely unlabeled, regardless of fruit temperature.”
t0“Developing Soybean Seeds”
Claim · model-written summary
Under anaerobic incubation, excised soybean embryos took up 30% less labeled sucrose than under aerobic conditions.
- Taxon
- soybeanGlycine maxt0
- Treatment
- anaerobic in vitro incubation conditionse0
- Outcome
- uptake of [(14)C]sucrose in excised embryose0
- Direction
- lesse0
- Value
- 30%e0
- Comparator
- aerobic in vitro conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“under anaerobic in vitro incubation conditions, uptake of [(14)C]sucrose in excised embryos was only 30% less than that in aerobic in vitro conditions.”
t0“Developing Soybean Seeds”
Claim · model-written summary
Anoxia appears to prevent sucrose efflux from soybean seed coat phloem and subsequent embryo uptake in intact fruit.
- Taxon
- soybeanGlycine maxt0
- Treatment
- anoxiae0
- Outcome
- sucrose efflux from the seed coat phloem and any subsequent uptake by the embryoe0
- Direction
- preventede0
- Qualifier
- within intact fruite0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The data suggest that, within intact fruit, anoxia prevented sucrose efflux from the seed coat phloem and any subsequent uptake by the embryo.”
t0“Developing Soybean Seeds”
Claim · model-written summary
Energy dependent phloem unloading may reflect membrane integrity or energy metabolism requirements in soybean seed coats.
- Taxon
- soybeanGlycine maxt0
- Outcome
- energy dependence of phloem unloadinge0
- Qualifier
- requirements for membrane integrity or energy metabolism in the companion cell-sieve element complexe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The demonstrated energy dependence of phloem unloading may reflect requirements for membrane integrity or energy metabolism in the companion cell-sieve element complex, consistent with a facilitated unloading process.”
t0“Developing Soybean Seeds”
Claim · model-written summary
Environmental regulation of soybean photosynthate import may occur both in embryos and at seed coat phloem terminals.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- seed coate0
- Outcome
- environmental regulation of importe0
- Qualifier
- at both the embryo level and at the phloem terminals within the seed coate0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“They imply that environmental regulation of import may occur at both the embryo level and at the phloem terminals within the seed coat.”
t0“Developing Soybean Seeds”
The same questions in other papers
in vitro · nutrient: content · Glycine max (soybean)Positive and negative results
6 in the opposite direction
- positive
- positive
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.