Paper · 1966 · Nematologica

Analysis of Host-Parasite Relationships of Root-Knot Nematodes By Single-Larva Inoculations of Excised Tomato Roots

Victor H. Dropkin; William R. Boone

11
claims
11
verbatim quotes
32
citations
1
research areas
—
Not open access

Abstract

This report describes a bacteria-free culture unit to be used in large numbers for detailed analysis of host-parasite relationships of Meloidogyne sp. The unit consists of an excised tomato root growing in agar medium in a test tube to which one nematode larva has been added. Almost every nematode which penetrates induces a gall; a high proportion of these galls have eggs externally after 3 weeks of incubation at 28° C. Galls appear from the 1st to the 9th day, eggs from the 18th to the 26th day, and larvae from the 25th to the 32nd day after inoculation. Infectivity of Meloidogyne incognita acrita was 53%. Variations in nutrient composition had no marked effects except that high potassium stimulated egg production without affecting incidence of gall formation; high concentrations of iodine increased brown discoloration of galls. Addition of juice from a resistant plant (Cornus florida) depressed the infectivity of M. incognita acrita for tomato roots. A resistant variety of tomato, Nemared, maintained its resistance to Meloidogyne in excised root culture.

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Claims 11

Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Most penetrating nematodes induced galls.

Interactor
nematodeNematodae0pathogen
Outcome
induces a galle0

Not reportedTaxon

Verbatim

e0

“Almost every nematode which penetrates induces a gall”

✓ located verbatim in the source · abstract, characters 277–330 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Many galls bore external eggs after three weeks.

Outcome
galls have eggs externallye0
Qualifier
after 3 weeks of incubation at 28° Ce0

Not reportedTaxon

Verbatim

e0

“a high proportion of these galls have eggs externally after 3 weeks of incubation at 28° C”

✓ located verbatim in the source · abstract, characters 332–422 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Galls appeared on days 1 through 9.

Outcome
Galls appeare0
Value
1st to the 9th daye0

Not reportedTaxon

Verbatim

e0

“Galls appear from the 1st to the 9th day”

✓ located verbatim in the source · abstract, characters 424–464 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Eggs appeared on days 18 through 26.

Outcome
eggse0
Value
18th to the 26th daye0

Not reportedTaxon

Verbatim

e0

“eggs from the 18th to the 26th day”

✓ located verbatim in the source · abstract, characters 466–500 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Larvae appeared on days 25 through 32.

Outcome
larvaee0
Value
25th to the 32nd daye0

Not reportedTaxon

Verbatim

e0

“larvae from the 25th to the 32nd day after inoculation”

✓ located verbatim in the source · abstract, characters 506–560 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Meloidogyne incognita acrita infectivity was 53%.

Interactor
Meloidogyne incognita acritaMeloidogyne incognitae0pathogen
Outcome
Infectivity of Meloidogyne incognita acritae0
Value
53%e0

Not reportedTaxon

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Meloidogyne incognita
Meloidogyne incognita · InteractorPhoto by William Wergin and Richard Sayre. Colorized by Stephen Ausmus. U.S. Department of Agriculture · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“Infectivity of Meloidogyne incognita acrita was 53%”

✓ located verbatim in the source · abstract, characters 562–613 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

High potassium stimulated nematode egg production.

Treatment
high potassiume0
Outcome
egg productione0
Direction
stimulatede0

Not reportedTaxonComparator

Verbatim

e0

“high potassium stimulated egg production without affecting incidence of gall formation”

✓ located verbatim in the source · abstract, characters 684–770 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

High potassium did not affect gall formation incidence.

Treatment
high potassiume0
Outcome
incidence of gall formatione0
Direction
without affectinge0

Not reportedTaxonComparator

Verbatim

e0

“high potassium stimulated egg production without affecting incidence of gall formation”

✓ located verbatim in the source · abstract, characters 684–770 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

High iodine increased brown gall discoloration.

Treatment
high concentrations of iodinee0
Outcome
brown discoloration of gallse0
Direction
increasede0

Not reportedTaxonComparator

Verbatim

e0

“high concentrations of iodine increased brown discoloration of galls”

✓ located verbatim in the source · abstract, characters 772–840 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Cornus florida juice reduced M. incognita acrita infectivity on tomato roots.

Treatment
juice from a resistant plant (Cornus florida)e0
Interactor
M. incognita acritaMeloidogyne incognitae0pathogen
Outcome
infectivity of M. incognita acritae0
Direction
depressede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗
Meloidogyne incognita
Meloidogyne incognita · InteractorPhoto by William Wergin and Richard Sayre. Colorized by Stephen Ausmus. U.S. Department of Agriculture · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“Addition of juice from a resistant plant (Cornus florida) depressed the infectivity of M. incognita acrita for tomato roots”

✓ located verbatim in the source · abstract, characters 842–965 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Nemared retained resistance in excised root culture.

Material
Nemarede0
Interactor
MeloidogyneMeloidogynee0pathogen
Outcome
resistance to Meloidogynee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗
Meloidogyne
Meloidogyne · Interactor · picture of the genus (one of its species)Photo by William Wergin and Richard Sayre. Colorized by Stephen Ausmus. U.S. Department of Agriculture · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“A resistant variety of tomato, Nemared, maintained its resistance to Meloidogyne in excised root culture”

✓ located verbatim in the source · abstract, characters 967–1071 · show in text ↑

diiodine · pest · Solanum lycopersicum (tomato)Results agree

2 papers: 0 positive, 2 negative or no effect

1 paper in the same direction as this paper

  1. negative
See all 2 papers →

potassium · disease · Solanum lycopersicum (tomato)Positive and negative results

19 papers: 15 positive, 4 negative or no effect

3 papers in the same direction as this paper · 15 in the opposite direction

  1. positive
  2. positive
  3. positive

15 papers more in this group

See all 19 papers →

potassium · pest · Solanum lycopersicum (tomato)Results agree

2 papers: 0 positive, 2 negative or no effect

1 paper in the same direction as this paper

  1. no effect
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.