Abstract
A beta-glucoronidase (GUS)-marked strain of Herbaspirillum seropedicae Z67 was inoculated onto rice seedling cvs. IR42 and IR72. Internal populations peaked at over 10(6) log CFU per gram of fresh weight by 5 to 7 days after inoculation (DAI) but declined to 10(3) to 10(4) log CFU per gram of fresh weight by 28 DAI. GUS staining was most intense on coleoptiles, lateral roots, and at the junctions of some of the main and lateral roots. Bacteria entered the roots via cracks at the points of lateral root emergence, with cv. IR72 appearing to be more aggressively infected than cv. IR42. H. seropedicae subsequently colonized the root intercellular spaces, aerenchyma, and cortical cells, with a few penetrating the stele to enter the vascular tissue. Xylem vessels in leaves and stems were extensively colonized at 2 DAI but, in later harvests (7 and 13 DAI), a host defense reaction was often observed. Dense colonies of H. seropedicae with some bacteria expressing nitrogenase Fe-protein were seen within leaf and stem epidermal cells, intercellular spaces, and substomatal cavities up until 28 DAI. Epiphytic bacteria were also seen. Both varieties showed nitrogenase activity but only with added C, and the dry weights of the inoculated plants were significantly increased. Only cv. IR42 showed a significant (approximately 30%) increase in N content above that of the uninoculated controls, and it also incorporated a significant amount of 15N2.
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Claims 13
Claim · model-written summary
Internal H. seropedicae populations peaked above 10(6) log CFU per gram by 5 to 7 days.
- Taxon
- Rice
Oryza sativat0 - Interactor
- Herbaspirillum seropedicae Z67Herbaspirillum seropedicaee1symbiont
- Outcome
- Internal populationse0
- Direction
- peakede0
- Value
- over 10(6) log CFU per gram of fresh weighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice Seedlings”
e0“Internal populations peaked at over 10(6) log CFU per gram of fresh weight by 5 to 7 days after inoculation (DAI)”
e1“Herbaspirillum seropedicae Z67”
Claim · model-written summary
Internal bacterial populations declined to 10(3) to 10(4) log CFU per gram by 28 days.
- Taxon
- RiceOryza sativat0
- Interactor
- Herbaspirillum seropedicae Z67Herbaspirillum seropedicaee1symbiont
- Outcome
- populationse2
- Direction
- declinede0
- Value
- 10(3) to 10(4) log CFU per gram of fresh weighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice Seedlings”
e0“declined to 10(3) to 10(4) log CFU per gram of fresh weight by 28 DAI”
e1“Herbaspirillum seropedicae Z67”
e2“Internal populations peaked at over 10(6) log CFU per gram of fresh weight by 5 to 7 days after inoculation (DAI)”
Claim · model-written summary
GUS staining was most intense on coleoptiles, lateral roots and junctions of roots.
- Taxon
- RiceOryza sativat0
- Organ / stage
- coleoptiles, lateral rootse0
- Interactor
- Herbaspirillum seropedicae Z67Herbaspirillum seropedicaee1symbiont
- Outcome
- GUS staininge0
- Direction
- most intensee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice Seedlings”
e0“GUS staining was most intense on coleoptiles, lateral roots, and at the junctions of some of the main and lateral roots”
e1“Herbaspirillum seropedicae Z67”
Claim · model-written summary
Bacteria entered roots through cracks at lateral root emergence; IR72 appeared more aggressively infected than IR42.
- Taxon
- RiceOryza sativat0
- Material
- cv. IR72e0
- Organ / stage
- rootse0
- Interactor
- Herbaspirillum seropedicae Z67Herbaspirillum seropedicaee1symbiont
- Outcome
- more aggressively infectede0
- Direction
- more aggressivelye0
- Comparator
- cv. IR42e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice Seedlings”
e0“Bacteria entered the roots via cracks at the points of lateral root emergence, with cv. IR72 appearing to be more aggressively infected than cv. IR42”
e1“Herbaspirillum seropedicae Z67”
Claim · model-written summary
H. seropedicae colonized root intercellular spaces, aerenchyma and cortical cells, and some entered the stele.
- Taxon
- RiceOryza sativat0
- Interactor
- H. seropedicaeHerbaspirillum seropedicaee0symbiont
- Outcome
- colonized the root intercellular spaces, aerenchyma, and cortical cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice Seedlings”
e0“H. seropedicae subsequently colonized the root intercellular spaces, aerenchyma, and cortical cells, with a few penetrating the stele to enter the vascular tissue”
Claim · model-written summary
Leaf and stem xylem vessels were extensively colonized at 2 days after inoculation.
- Taxon
- RiceOryza sativat0
- Organ / stage
- Xylem vessels in leaves and stemse0
- Interactor
- Herbaspirillum seropedicae Z67Herbaspirillum seropedicaee1symbiont
- Outcome
- extensively colonizede0
- Qualifier
- 2 DAIe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice Seedlings”
e0“Xylem vessels in leaves and stems were extensively colonized at 2 DAI”
e1“Herbaspirillum seropedicae Z67”
Claim · model-written summary
A host defense reaction was often observed at 7 and 13 days.
- Taxon
- RiceOryza sativat0
- Interactor
- Herbaspirillum seropedicae Z67Herbaspirillum seropedicaee1symbiont
- Outcome
- host defense reactione0
- Qualifier
- 7 and 13 DAIe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice Seedlings”
e0“in later harvests (7 and 13 DAI), a host defense reaction was often observed”
e1“Herbaspirillum seropedicae Z67”
Claim · model-written summary
Dense H. seropedicae colonies included bacteria expressing nitrogenase Fe-protein through 28 days.
- Taxon
- RiceOryza sativat0
- Organ / stage
- leaf and stem epidermal cellse0
- Interactor
- H. seropedicaeHerbaspirillum seropedicaee0symbiont
- Gene
- nitrogenase Fe-proteine0
- Outcome
- bacteria expressing nitrogenase Fe-proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice Seedlings”
e0“Dense colonies of H. seropedicae with some bacteria expressing nitrogenase Fe-protein were seen within leaf and stem epidermal cells, intercellular spaces, and substomatal cavities up until 28 DAI”
Claim · model-written summary
Epiphytic bacteria were also observed.
- Taxon
- RiceOryza sativat0
- Interactor
- Herbaspirillum seropedicae Z67Herbaspirillum seropedicaee1symbiont
- Outcome
- Epiphytic bacteriae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice Seedlings”
e0“Epiphytic bacteria were also seen”
e1“Herbaspirillum seropedicae Z67”
Claim · model-written summary
Both rice varieties showed nitrogenase activity only when carbon was added.
- Taxon
- RiceOryza sativat0
- Interactor
- Herbaspirillum seropedicae Z67Herbaspirillum seropedicaee1symbiont
- Outcome
- nitrogenase activitye0
- Qualifier
- only with added Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice Seedlings”
e0“Both varieties showed nitrogenase activity but only with added C”
e1“Herbaspirillum seropedicae Z67”
Claim · model-written summary
Inoculated rice plants had significantly increased dry weight.
- Taxon
- riceOryza sativae1
- Treatment
- Herbaspirillum seropedicae Z67 was inoculatede1
- Interactor
- Herbaspirillum seropedicae Z67Herbaspirillum seropedicaee1symbiont
- Outcome
- dry weightse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice Seedlings”
e0“the dry weights of the inoculated plants were significantly increased”
e1“A beta-glucoronidase (GUS)-marked strain of Herbaspirillum seropedicae Z67 was inoculated onto rice seedling cvs. IR42 and IR72”
Claim · model-written summary
Only IR42 had about 30% higher nitrogen content than uninoculated controls.
- Taxon
- riceOryza sativae1
- Material
- cv. IR42e0
- Treatment
- Herbaspirillum seropedicae Z67 was inoculatede1
- Interactor
- Herbaspirillum seropedicae Z67Herbaspirillum seropedicaee1symbiont
- Outcome
- N contente0
- Direction
- increasee0
- Value
- approximately 30%e0
- Comparator
- uninoculated controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice Seedlings”
e0“Only cv. IR42 showed a significant (approximately 30%) increase in N content above that of the uninoculated controls”
e1“Herbaspirillum seropedicae Z67 was inoculated onto rice seedling cvs. IR42 and IR72”
Claim · model-written summary
IR42 incorporated a significant amount of 15N2.
- Taxon
- riceOryza sativae1
- Material
- cv. IR42e0
- Interactor
- Herbaspirillum seropedicae Z67Herbaspirillum seropedicaee1symbiont
- Outcome
- incorporated a significant amount of 15N2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Only cv. IR42 showed a significant (approximately 30%) increase in N content above that of the uninoculated controls, and it also incorporated a significant amount of 15N2”
e1“Herbaspirillum seropedicae Z67 was inoculated onto rice seedling cvs. IR42 and IR72”
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