Abstract
Purified, right side‐out plasmalemma vesicles were isolated from 7‐day‐old roots of dark‐grown wheat ( Triticum aestivum L. cv. Drabant) by aqueous polymer two‐phase partitioning. The oxygen consumption by these vesicles at pH 6.5 in the presence of 1 m M NADH [12–29 nmol (mg protein) −1 min −1 ] was 66% inhibited by 1 m M KCN and ca 40% by 1 m M EDTA. It was unaffected by rotenone, antimycin A, carbonyl cyanide trifluoromethoxyphenylhydrazone (FCCP), mersalyl, chlorotetracycline + Ca 2+ , and EGTA. Salicylhydroxamic acid (SHAM) and its analogue, m ‐chlorobenzhydroxamic acid, stimulated the rate of oxygen consumption 10–20 fold in the presence of 1 m M NAD(P)H with an apparent K m (SHAM) of ca 40 μ M (with NADH). The dependence of O 2 consumption on NADH concentration in the presence of SHAM (2 m M ) was sigmoidal, possibly due to endogenous catalase activity, and half‐maximal rate was obtained at 1.5 m M . In the absence of SHAM the rate increased with increasing acidity and no pH optimum was detectable between pH 4.5 and 8.5. In the presence of SHAM an optimum was observed at pH 6.5 and 0.8 mol of H 2 O 2 was produced for every 1 mol O 2 consumed. Endogenous catalase converted this H 2 O 2 to O 2 and after complete conversion the stoichiometry was 2 mol NADH consumed for every mol O 3 . SHAM was not consumed in the reaction. The possible involvement of a cytochrome P‐450/420 system is discussed.
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Claims 11
Claim · model-written summary
Salicylhydroxamic acid and its analogue stimulated oxygen consumption 10–20 fold in the presence of NAD(P)H.
- Taxon
- Triticum aestivum L.
Triticum aestivume0 - Treatment
- Salicylhydroxamic acid (SHAM)e1
- Outcome
- rate of oxygen consumptione1
- Direction
- stimulatede1
- Value
- 10–20 folde1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dark‐grown wheat ( Triticum aestivum L. cv. Drabant)”
e1“Salicylhydroxamic acid (SHAM) and its analogue, m ‐chlorobenzhydroxamic acid, stimulated the rate of oxygen consumption 10–20 fold in the presence of 1 m M NAD(P)H”
Claim · model-written summary
KCN inhibited oxygen consumption by 66% in wheat root plasmalemma vesicles.
- Taxon
- Triticum aestivum L.Triticum aestivume0
- Treatment
- 1 m M KCNe2
- Outcome
- oxygen consumptione2
- Direction
- inhibitede2
- Value
- 66%e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dark‐grown wheat ( Triticum aestivum L. cv. Drabant)”
e2“The oxygen consumption by these vesicles at pH 6.5 in the presence of 1 m M NADH [12–29 nmol (mg protein) −1 min −1 ] was 66% inhibited by 1 m M KCN and ca 40% by 1 m M EDTA”
Claim · model-written summary
EDTA inhibited oxygen consumption by about 40% in wheat root plasmalemma vesicles.
- Taxon
- Triticum aestivum L.Triticum aestivume0
- Treatment
- 1 m M EDTAe2
- Outcome
- oxygen consumptione2
- Direction
- inhibitede2
- Value
- ca 40%e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dark‐grown wheat ( Triticum aestivum L. cv. Drabant)”
e2“The oxygen consumption by these vesicles at pH 6.5 in the presence of 1 m M NADH [12–29 nmol (mg protein) −1 min −1 ] was 66% inhibited by 1 m M KCN and ca 40% by 1 m M EDTA”
Claim · model-written summary
Rotenone, antimycin A and FCCP did not affect oxygen consumption by wheat root plasmalemma vesicles.
- Taxon
- Triticum aestivum L.Triticum aestivume0
- Treatment
- rotenone, antimycin A, carbonyl cyanide trifluoromethoxyphenylhydrazone (FCCP)e3
- Outcome
- oxygen consumptione2
- Direction
- unaffectede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dark‐grown wheat ( Triticum aestivum L. cv. Drabant)”
e2“The oxygen consumption by these vesicles at pH 6.5 in the presence of 1 m M NADH [12–29 nmol (mg protein) −1 min −1 ] was 66% inhibited by 1 m M KCN and ca 40% by 1 m M EDTA”
e3“It was unaffected by rotenone, antimycin A, carbonyl cyanide trifluoromethoxyphenylhydrazone (FCCP), mersalyl, chlorotetracycline + Ca 2+ , and EGTA”
Claim · model-written summary
Mersalyl, chlorotetracycline with Ca 2+, and EGTA did not affect oxygen consumption by wheat root plasmalemma vesicles.
- Taxon
- Triticum aestivum L.Triticum aestivume0
- Treatment
- mersalyl, chlorotetracycline + Ca 2+ , and EGTAe3
- Outcome
- oxygen consumptione2
- Direction
- unaffectede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dark‐grown wheat ( Triticum aestivum L. cv. Drabant)”
e2“The oxygen consumption by these vesicles at pH 6.5 in the presence of 1 m M NADH [12–29 nmol (mg protein) −1 min −1 ] was 66% inhibited by 1 m M KCN and ca 40% by 1 m M EDTA”
e3“It was unaffected by rotenone, antimycin A, carbonyl cyanide trifluoromethoxyphenylhydrazone (FCCP), mersalyl, chlorotetracycline + Ca 2+ , and EGTA”
Claim · model-written summary
The apparent Km for SHAM was about 40 μM with NADH.
- Taxon
- Triticum aestivum L.Triticum aestivume0
- Outcome
- apparent K m (SHAM)e4
- Value
- ca 40 μMe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dark‐grown wheat ( Triticum aestivum L. cv. Drabant)”
e4“apparent K m (SHAM) of ca 40 μM (with NADH)”
Claim · model-written summary
The dependence of oxygen consumption on NADH concentration with SHAM was sigmoidal, with half-maximal rate at 1.5 mM NADH.
- Taxon
- Triticum aestivum L.Triticum aestivume0
- Outcome
- dependence of O 2 consumption on NADH concentratione5
- Value
- 1.5 m Me5
- Qualifier
- sigmoidale5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dark‐grown wheat ( Triticum aestivum L. cv. Drabant)”
e5“The dependence of O 2 consumption on NADH concentration in the presence of SHAM (2 m M ) was sigmoidal, possibly due to endogenous catalase activity, and half‐maximal rate was obtained at 1.5 m M”
Claim · model-written summary
With SHAM present, the pH optimum of the reaction was pH 6.5.
- Taxon
- Triticum aestivum L.Triticum aestivume0
- Outcome
- optimume6
- Value
- pH 6.5e6
- Qualifier
- In the presence of SHAMe6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dark‐grown wheat ( Triticum aestivum L. cv. Drabant)”
e6“In the presence of SHAM an optimum was observed at pH 6.5”
Claim · model-written summary
About 0.8 mol of H2O2 was produced per mol of O2 consumed in the presence of SHAM.
- Taxon
- Triticum aestivum L.Triticum aestivume0
- Outcome
- H 2 O 2 was producede7
- Value
- 0.8 mole7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dark‐grown wheat ( Triticum aestivum L. cv. Drabant)”
e7“0.8 mol of H 2 O 2 was produced for every 1 mol O 2 consumed”
Claim · model-written summary
After complete catalase conversion of H2O2, the stoichiometry was 2 mol NADH consumed per mol O2.
- Taxon
- Triticum aestivum L.Triticum aestivume0
- Outcome
- stoichiometrye1
- Value
- 2 mol NADHe1
- Qualifier
- after complete conversione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dark‐grown wheat ( Triticum aestivum L. cv. Drabant)”
e1“Endogenous catalase converted this H 2 O 2 to O 2 and after complete conversion the stoichiometry was 2 mol NADH consumed for every mol O 3”
Claim · model-written summary
SHAM was not consumed in the reaction.
- Taxon
- Triticum aestivum L.Triticum aestivume0
- Outcome
- SHAM was not consumede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dark‐grown wheat ( Triticum aestivum L. cv. Drabant)”
e2“SHAM was not consumed in the reaction”
The same questions in other papers
rotenone · pest · Triticum aestivum (wheat)Positive and negative results
1 in the opposite direction
- positive
calcium(2+) · pest · Triticum aestivum (wheat)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.