Abstract
Based on information from the Arabidopsis model system, a putative transcriptional activator of cuticle formation (TaSHN1) was selected among the expressed sequence tags in wheat (Triticum aestivum L.). RT-PCR indicated the preferential expression of this gene in the basal, but not in the middle parts of wheat leaves. This leaf region is a likely site of cuticle formation in cereals. TaSHN1 was cloned and expressed in Arabidopsis, resulting in shiny leaf surfaces and the overproliferation of cuticular material as observed by electron microscopy. Unlike the Arabidopsis WAX INDUCER/SHINE1 (WIN/SHN1) gene, TaSHN1 triggered disorganized cuticular ultrastructure in the transgenic leaves, with the continuous layers replaced by large electrodense bodies embedded in amorphous lipid material. Toluidine blue staining and dark-adapted water release indicated increased cuticular permeability in TaSHN1-expressing Arabidopsis leaves. The expression of TaSHN1 resulted in a moderate decrease of the total number of stomata per unit leaf area in comparison with the wild type. Drought tolerance of Arabidopsis was unaffected by the transgene. The data indicate that this putative wheat orthologue of WIN/SHN transcription factors (TaSHN1) elicited both overlapping and new, distinctive phenotypes compared to other WIN/SHN-overexpressing plants. TaSHN1 transgenic Arabidopsis lines should provide a rich source of material for further comparative biochemical, physiological, and genetic studies.
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Claims 6
Claim · model-written summary
TaSHN1 expression was preferential in the basal parts of wheat leaves and not in the middle parts.
- Taxon
- wheat
Triticum aestivume2 - Organ / stage
- basale2
- Gene
- TaSHN1e1
- Outcome
- expressione2
- Direction
- preferentiale2
- Comparator
- middle parts of wheat leavese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a putative transcriptional activator of cuticle formation (TaSHN1)”
e2“RT-PCR indicated the preferential expression of this gene in the basal, but not in the middle parts of wheat leaves”
Claim · model-written summary
Expression of TaSHN1 in Arabidopsis gave shiny leaf surfaces and overproliferation of cuticular material.
- Taxon
- Arabidopsis
Arabidopsise1 - Treatment
- TaSHN1e1
- Gene
- TaSHN1e1
- Outcome
- shiny leaf surfaces and the overproliferation of cuticular materiale1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“TaSHN1 was cloned and expressed in Arabidopsis, resulting in shiny leaf surfaces and the overproliferation of cuticular material”
Claim · model-written summary
Unlike Arabidopsis WIN/SHN1, TaSHN1 in Arabidopsis triggered disorganized cuticular ultrastructure.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- TaSHN1e1
- Gene
- TaSHN1e1
- Outcome
- disorganized cuticular ultrastructuree1
- Qualifier
- continuous layers replaced by large electrodense bodies embedded in amorphous lipid materiale1
- Comparator
- WIN/SHN1e1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Unlike the Arabidopsis WAX INDUCER/SHINE1 (WIN/SHN1) gene, TaSHN1 triggered disorganized cuticular ultrastructure in the transgenic leaves, with the continuous layers replaced by large electrodense bodies embedded in amorphous lipid material”
Claim · model-written summary
TaSHN1-expressing Arabidopsis leaves showed increased cuticular permeability.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- TaSHN1-expressinge1
- Gene
- TaSHN1e1
- Outcome
- cuticular permeabilitye1
- Direction
- increasede1
- Qualifier
- Toluidine blue staining and dark-adapted water releasee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Toluidine blue staining and dark-adapted water release indicated increased cuticular permeability in TaSHN1-expressing Arabidopsis leaves”
Claim · model-written summary
TaSHN1 expression moderately decreased the total number of stomata per unit leaf area compared with the wild type.
- Taxon
- ArabidopsisArabidopsise2
- Treatment
- expression of TaSHN1e1
- Gene
- TaSHN1e1
- Outcome
- total number of stomata per unit leaf areae1
- Direction
- decreasee1
- Qualifier
- moderatee1
- Comparator
- wild typee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The expression of TaSHN1 resulted in a moderate decrease of the total number of stomata per unit leaf area in comparison with the wild type”
e2“TaSHN1 was cloned and expressed in Arabidopsis”
Claim · model-written summary
Drought tolerance of Arabidopsis was unaffected by the transgene.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- transgenee1
- Outcome
- Drought tolerancee1
- Direction
- unaffectede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Drought tolerance of Arabidopsis was unaffected by the transgene”
The same questions in other papers
transgenic · tolerance: abiotic · ArabidopsisPositive and negative results
1 paper in the same direction as this paper · 19 in the opposite direction
- positive
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See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.