Abstract
Virus-induced gene silencing (VIGS) is increasingly being used as a reverse genetics tool to study functions of specific plant genes. It is especially useful for plants, such as soybean, that are recalcitrant to transformation. Previously, Bean pod mottle virus (BPMV) was shown to be an effective VIGS vector for soybean. However, the reported BPMV vector requires in vitro RNA transcription and inoculation, which is not reliable or amenable to high-throughput applications. To increase the efficiency of the BPMV vector for soybean functional genomics, a DNA-based version was developed. Reported here is the construction of a Cauliflower mosaic virus 35S promoter-driven BPMV vector that is efficient for the study of soybean gene function. The selection of a mild rather than a severe BPMV strain greatly reduced the symptom interference caused by virus infection. The DNA-based BPMV vector was used to silence soybean homologues of genes involved in plant defense, translation, and the cytoskeleton in shoots and in roots. VIGS of the Actin gene resulted in reduced numbers of Soybean mosaic virus infection foci. The results demonstrate the utility of this new vector as an efficient tool for a wide range of applications for soybean functional genomics.
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Claims 3
Claim · model-written summary
A mild rather than a severe BPMV strain greatly reduced the symptom interference caused by virus infection in soybean.
- Taxon
- Soybean
Glycine maxt0 - Treatment
- selection of a mild rather than a severe BPMV straine0
- Interactor
- BPMVBean pod mottle viruse0pathogen
- Outcome
- symptom interference caused by virus infectione0
- Direction
- reducede0
- Comparator
- a severe BPMV straine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The selection of a mild rather than a severe BPMV strain greatly reduced the symptom interference caused by virus infection”
t0“Efficient DNA-Based Viral Gene Silencing Vector for Soybean”
Claim · model-written summary
A DNA-based BPMV vector under a CaMV 35S promoter is efficient for soybean gene-function studies, compared with the reported BPMV vector that needs in vitro RNA transcription.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- efficient for the study of soybean gene functione1
- Comparator
- the reported BPMV vectore2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a Cauliflower mosaic virus 35S promoter-driven BPMV vector that is efficient for the study of soybean gene function”
e2“the reported BPMV vector requires in vitro RNA transcription and inoculation”
t0“Efficient DNA-Based Viral Gene Silencing Vector for Soybean”
Claim · model-written summary
VIGS of the Actin gene reduced the numbers of Soybean mosaic virus infection foci in soybean.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- VIGS of the Actin genee3
- Interactor
- Soybean mosaic virus
Soybean mosaic viruse3pathogen - Gene
- Actine3
- Outcome
- numbers of Soybean mosaic virus infection focie3
- Direction
- reducede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“VIGS of the Actin gene resulted in reduced numbers of Soybean mosaic virus infection foci”
t0“Efficient DNA-Based Viral Gene Silencing Vector for Soybean”
The same questions in other papers
loss of function · Soybean mosaic virus · Glycine max (soybean)Positive and negative results
1 paper in the same direction as this paper · 4 in the opposite direction
- negative
- negative
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.