Abstract
Glyphosate ( N ‐phosphonomethyl‐glycine) is the active ingredient the nonselective herbicide Roundup. The sensitivity of crop plants to glyphosate has limited its in‐season use as a postemergence herbicide. The extension of the use of Roundup herbicide to allow in‐season application in major crops such as soybeans [ Glycine max (L.) Merr.] would provide new weed control options for farmers. A glyphosate‐tolerant soybean line, 40‐3‐2, was obtained through expression of the bacterial 5‐enolpyruvylshikimate‐3‐phosphate synthase (EPSP synthase, EPSPS) enzyme from Agrobacterium sp. strain CP4. Line 40‐3‐2 is highly tolerant to glyphosate, showing no visual injury after application of up to 1.68 kg acid equivalent (a.e.) ha −1 of glyphosate under field conditions. Molecular characterization studies determined that the single genetic insert in line 40‐3‐2 contains only a portion of the cauliflower mosaic virus 35S promoter (P‐E35S), the Petunia hybrida EPSPS chloroplast transit peptide (CTP), the CP4 EPSPS gene, and a portion of the 3' nontranslated region of the nopaline synthase gene (NOS 3') terminator. Inheritance studies have shown that the transgene behaves as a single dominant gene and is stable over several generations.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 4
Claim · model-written summary
Soybean line 40‐3‐2 was obtained by expressing bacterial EPSPS.
- Taxon
- soybean
Glycine maxe0 - Material
- 40‐3‐2e0
- Gene
- 5‐enolpyruvylshikimate‐3‐phosphate synthasee0
- Outcome
- glyphosate‐tolerant soybean linee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A glyphosate‐tolerant soybean line, 40‐3‐2, was obtained through expression of the bacterial 5‐enolpyruvylshikimate‐3‐phosphate synthase (EPSP synthase, EPSPS) enzyme from Agrobacterium sp. strain CP4.”
Claim · model-written summary
Line 40‐3‐2 showed no visual injury after glyphosate application up to 1.68 kg acid equivalent per hectare in the field.
- Taxon
- SoybeanGlycine maxt0
- Material
- 40‐3‐2e0
- Treatment
- application of up to 1.68 kg acid equivalent (a.e.) ha −1 of glyphosatee0
- Outcome
- no visual injurye0
- Setting
- field conditionse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Line 40‐3‐2 is highly tolerant to glyphosate, showing no visual injury after application of up to 1.68 kg acid equivalent (a.e.) ha −1 of glyphosate under field conditions.”
t0“Soybean Line”
Claim · model-written summary
The single insert in line 40‐3‐2 contained portions of P‐E35S and NOS 3′ and the Petunia CTP and CP4 EPSPS gene.
- Taxon
- SoybeanGlycine maxt0
- Material
- 40‐3‐2e0
- Gene
- CP4 EPSPS genee0
- Outcome
- single genetic inserte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Molecular characterization studies determined that the single genetic insert in line 40‐3‐2 contains only a portion of the cauliflower mosaic virus 35S promoter (P‐E35S), the Petunia hybrida EPSPS chloroplast transit peptide (CTP), the CP4 EPSPS gene, and a portion of the 3' nontranslated region of the nopaline synthase gene (NOS 3') terminator.”
t0“Soybean Line”
Claim · model-written summary
The transgene in line 40‐3‐2 behaved as a single dominant gene and was stable over several generations.
- Taxon
- SoybeanGlycine maxt0
- Gene
- transgenee0
- Outcome
- single dominant gene and is stable over several generationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inheritance studies have shown that the transgene behaves as a single dominant gene and is stable over several generations.”
t0“Soybean Line”
The same questions in other papers
glyphosate · crop injury · Glycine max (soybean)Results differ
9 papers in the same direction as this paper · 22 in the opposite direction · 1 reporting no difference from an alternative
- negative
- no effect
- negative
29 papers more in this group
See all 33 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.