Abstract
Plant transient expression using virus-based vectors is advantageous when high level of gene expression is desired within a short time. In this study, a new system, named “air-brush,” has been developed to facilitate a scale-up production of recombinant proteins in plants. GFP was expressed successfully in Nicotiana benthamiana (Nb) plants by air-brushing an Agrobacterium suspension that contained the TMV-based vector p35S-30B-GFP. Key factors influencing the gene expression were optimized, including the Agrobacterium cell density, seedling age, and the growth temperature of plant materials. In addition, the pharmaceutical protein human acidic fibroblast growth factor (ha FGF) was also expressed in Nb plants by the air-brush system. The results demonstrated that using this system is highly advantageous; it is convenient, quick, easily scaled-up, and has a higher expression efficiency than leaf infiltration.
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Claims 3
Claim · model-written summary
The air-brush system has a higher expression efficiency than leaf infiltration and is convenient, quick and easily scaled up.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Outcome
- expression efficiencye1
- Direction
- highere1
- Qualifier
- convenient, quick, easily scaled-upe1
- Comparator
- leaf infiltratione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GFP was expressed successfully in Nicotiana benthamiana (Nb) plants by air-brushing an Agrobacterium suspension”
e1“it is convenient, quick, easily scaled-up, and has a higher expression efficiency than leaf infiltration”
Claim · model-written summary
GFP was expressed successfully in Nicotiana benthamiana plants by air-brushing an Agrobacterium suspension.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Treatment
- air-brushing an Agrobacterium suspensione0
- Gene
- GFPe0
- Outcome
- GFP was expressed successfullye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GFP was expressed successfully in Nicotiana benthamiana (Nb) plants by air-brushing an Agrobacterium suspension”
Claim · model-written summary
The human acidic fibroblast growth factor (ha FGF) was expressed in Nicotiana benthamiana plants by the air-brush system.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Treatment
- by the air-brush systeme2
- Gene
- human acidic fibroblast growth factor (ha FGF)e2
- Outcome
- expressed in Nb plantse2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GFP was expressed successfully in Nicotiana benthamiana (Nb) plants by air-brushing an Agrobacterium suspension”
e2“the pharmaceutical protein human acidic fibroblast growth factor (ha FGF) was also expressed in Nb plants by the air-brush system”
The same questions in other papers
Agrobacterium · molecular: expression · Nicotiana benthamianaPositive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.