Abstract
The effects of cold on protein and RNA metabolism in leaves of rice (Oryza sativa L.) seedlings were investigated. Treatment with a diurnal cycle of 15/10 degrees C or 11/6 degrees C for up to 1 week resulted in progressive changes in the protein synthesis pattern after in vivo labeling of intact rice leaves with [(35)S]methionine. These changes were reversed when the seedlings were returned to normal growth temperatures. While de novo accumulation of several abundant proteins was suppressed, some polypeptides were consistently found to be cold-induced. Synthesis of ribulose 1,5-bisphosphate carboxylase (Rubisco) was drastically reduced after 7 days of cold. Using immunoprecipitation of Rubisco, evidence was obtained that the suppression was greater for the small subunit (over 90%) than for the large subunit (80%), indicating a partial loss of coordination in their synthesis. Preformed Rubisco as well as other cold-suppressed proteins were stable for up to 7 days at 11/6 degrees C. Cold-sensitive rice cultivars responded with similar but more drastic changes in the protein synthesis pattern when compared to cold-tolerant varieties. The suppression of Rubisco synthesis by cold was shown to result from reduced levels of the mRNAs encoding both subunits; their decrease paralleled the lower protein synthesis of each. The levels of other chloroplast-encoded mRNAs, especially psaB, and of the nuclear encoded chlorophyll a/b binding protein, also strongly decreased in the cold, whereas the transcripts of the mitochondrial genes apt9, coxIII, and most nuclear genes analyzed were unaffected or only slightly reduced. These data indicate that some chloroplast functions are disturbed during cold stress. One nuclear gene known to be induced by water stress and ABA (Rab21) was also found to be induced by cold treatment.
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Claims 11
Claim · model-written summary
Cold progressively changed leaf protein synthesis patterns, reversibly on warming.
- Taxon
- rice
Oryza sativae0 - Organ / stage
- leavese0
- Treatment
- 15/10 degrees C or 11/6 degrees Ce0
- Outcome
- protein synthesis patterne0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment with a diurnal cycle of 15/10 degrees C or 11/6 degrees C for up to 1 week resulted in progressive changes in the protein synthesis pattern after in vivo labeling of intact rice leaves with [(35)S]methionine.”
Claim · model-written summary
Cold suppressed accumulation of several abundant proteins.
- Taxon
- riceOryza sativat0
- Outcome
- de novo accumulation of several abundant proteinse0
- Direction
- suppressede0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“While de novo accumulation of several abundant proteins was suppressed”
t0“Rice Leaves”
Claim · model-written summary
Cold induced some polypeptides.
- Taxon
- riceOryza sativat0
- Treatment
- colde0
- Outcome
- some polypeptidese0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“some polypeptides were consistently found to be cold-induced”
t0“Rice Leaves”
Claim · model-written summary
After seven cold days, Rubisco synthesis fell more than 90% for the small subunit.
- Taxon
- riceOryza sativat0
- Gene
- small subunite0
- Outcome
- suppressione0
- Direction
- greatere0
- Value
- over 90%e0
- Comparator
- large subunite0
Not reportedTreatment
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the suppression was greater for the small subunit (over 90%) than for the large subunit (80%)”
t0“Rice Leaves”
Claim · model-written summary
After seven cold days, Rubisco large-subunit synthesis was suppressed by 80%.
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the suppression was greater for the small subunit (over 90%) than for the large subunit (80%)”
t0“Rice Leaves”
Claim · model-written summary
Preformed Rubisco and other cold-suppressed proteins remained stable for seven days at 11/6 degrees C.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Preformed Rubisco as well as other cold-suppressed proteins were stable for up to 7 days at 11/6 degrees C.”
t0“Rice Leaves”
Claim · model-written summary
Cold-sensitive cultivars had more drastic synthesis-pattern changes than cold-tolerant varieties.
- Taxon
- riceOryza sativae0
- Material
- Cold-sensitive rice cultivarse0
- Outcome
- changes in the protein synthesis patterne0
- Direction
- more drastice0
- Comparator
- cold-tolerant varietiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cold-sensitive rice cultivars responded with similar but more drastic changes in the protein synthesis pattern when compared to cold-tolerant varieties.”
Claim · model-written summary
Cold reduced mRNAs encoding both Rubisco subunits, paralleling reduced protein synthesis.
- Taxon
- riceOryza sativat0
- Treatment
- colde0
- Gene
- Rubiscoe0
- Outcome
- suppression of Rubisco synthesise0
- Qualifier
- reduced levels of the mRNAs encoding both subunitse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The suppression of Rubisco synthesis by cold was shown to result from reduced levels of the mRNAs encoding both subunits; their decrease paralleled the lower protein synthesis of each.”
t0“Rice Leaves”
Claim · model-written summary
Cold strongly decreased psaB and chlorophyll a/b binding protein transcripts.
- Taxon
- riceOryza sativat0
- Treatment
- colde0
- Gene
- psaBe0
- Outcome
- levels of other chloroplast-encoded mRNAse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The levels of other chloroplast-encoded mRNAs, especially psaB, and of the nuclear encoded chlorophyll a/b binding protein, also strongly decreased in the cold”
t0“Rice Leaves”
Claim · model-written summary
Mitochondrial apt9 and coxIII and most nuclear transcripts were unaffected or slightly reduced by cold.
- Taxon
- riceOryza sativat0
- Gene
- apt9, coxIIIe0
- Outcome
- transcriptse0
- Direction
- unaffected or only slightly reducede0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transcripts of the mitochondrial genes apt9, coxIII, and most nuclear genes analyzed were unaffected or only slightly reduced.”
t0“Rice Leaves”
Claim · model-written summary
Cold induced Rab21 expression.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One nuclear gene known to be induced by water stress and ABA (Rab21) was also found to be induced by cold treatment.”
t0“Rice Leaves”
The same questions in other papers
cold · molecular: metabolites · Oryza sativa (rice)Positive and negative results
9 papers in the same direction as this paper · 11 in the opposite direction
- positive
- positive
- positive
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See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.