Paper · 2006 · Journal of Agricultural and Food Chemistry

Quantification of Jasmonic Acid by SPME in Tomato Plants Stressed by Ozone

Claudia Zadra; Andrea Borgogni; Cesare Marucchini

4
claims
8
verbatim quotes
41
citations
2
research areas
—
Not open access

Abstract

Jasmonates are signalling molecules induced in plants as a response to various biotic and/or abiotic stresses. As ozone is known to activate defense responses in plants, we have monitored the concentration of jasmonic acid in tomato leaves during and after an acute exposure to this abiotic elicitor. In this experiment, we observed that the maximum induction of jasmonic acid in O3-fumigated plants occurred 9 h after the end of treatment and the concentration of jasmonic acid in stressed plants increased 13-fold. However, the level of endogenous methyl-jasmonate was constant during the observed period. The extraction and quantification of jasmonic acid as its methyl ester was performed by headspace-solid-phase microextraction (or HS-SPME) in combination with GC-FID and GC-MS. The sensitivity (LOD = 2 ng/g) of this method permitted the detection and quantification of jasmonic acid present in plant tissues at very low concentrations.

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Claims 4

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Jasmonic acid induction in ozone-fumigated tomato peaked 9 h after treatment.

Treatment
O3-fumigatede0
Outcome
maximum induction of jasmonic acide0
Direction
maximume0
Value
9 he0

Not reportedOrgan / stage

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the maximum induction of jasmonic acid in O3-fumigated plants occurred 9 h after the end of treatment”

✓ located verbatim in the source · abstract, characters 338–439 · show in text ↑
t0

“Tomato Plants”

✓ located verbatim in the source · title, characters 43–56 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Jasmonic acid concentration increased 13-fold in stressed tomato.

Treatment
Ozonet0
Outcome
concentration of jasmonic acide0
Direction
increasede0
Value
13-folde0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the concentration of jasmonic acid in stressed plants increased 13-fold”

✓ located verbatim in the source · abstract, characters 444–515 · show in text ↑
t0

“Tomato Plants Stressed by Ozone”

✓ located verbatim in the source · title, characters 43–74 · show in text ↑
Observationobservedoriginal resultno effectmeasured

Claim · model-written summary

Endogenous methyl-jasmonate remained constant during observation.

Outcome
level of endogenous methyl-jasmonatee0
Direction
constante0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the level of endogenous methyl-jasmonate was constant during the observed period”

✓ located verbatim in the source · abstract, characters 526–606 · show in text ↑
t0

“Tomato Plants”

✓ located verbatim in the source · title, characters 43–56 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

The method detected jasmonic acid in plant tissues at an LOD of 2 ng/g.

Outcome
detection and quantification of jasmonic acide0
Value
2 ng/ge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The sensitivity (LOD = 2 ng/g) of this method permitted the detection and quantification of jasmonic acid present in plant tissues at very low concentrations.”

✓ located verbatim in the source · abstract, characters 785–943 · show in text ↑
t0

“Tomato Plants”

✓ located verbatim in the source · title, characters 43–56 · show in text ↑

ozone · physiology: hormones · Solanum lycopersicum (tomato)Positive and negative results

4 papers: 2 positive, 2 negative or no effect

1 paper in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. no effect
  3. positive
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.