Paper · 1967 · Journal of the American Oil Chemists Society

Limiting temperature and relative humidity for growth and production of aflatoxin and free fatty acids byAspergillus flavus in sterile peanuts

Urban L. Diener; Norman D. Davis

9
claims
18
verbatim quotes
93
citations
2
research areas
—
Not open access

Abstract

Abstract Sound mature kernels, broken mature kernels, immature kernels, and unshelled Early Runner peanuts were heat‐treated in controlled environment cabinets and inoculated with spores of Aspergillus flavus . Treatments were incubated at 97‐99% relative humidity at different temperatures ranging from 5 to 55C and also at 30C with relative humidities ranging from 55 to 99%. Samples were removed after 7 and 21 days and assayed for aflatoxin, free fatty acids, and peanut kernel moisture. The limiting relative humidity for aflatoxin production by A. flavus was 85ŷ1% relative humidity for 21 days at 30C. The limiting low temperature for visible growth and aflatoxin production by the fungus was 13ŷ1C for 21 days at 97‐99% relative humidity. Damaged kernels, however, developed some afllatoxin in 21 days at 12C. The maximum temperature for aflatoxin production was 41.5ŷ1.5C for 21 days at 97‐99% relative humidity. Fungus growth and sporulation at 43C were equal to that at 40C, but no aflatoxin was produced. Moisture content of immature kernels was higher at equilibrium with the same relative humidity than the moisture content of sound mature kernels, damaged kernels, or kernels from unshelled peanuts. There appeared to be no proportional quantitative correlation between synthesis of aflatoxin and production of free fatty acids in nonliving peanuts, but no aflatoxin was produced without a simultaneous increase in free fatty acids.

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Claims 9

Observationobservedoriginal resultmeasured

Claim · model-written summary

The limiting relative humidity for aflatoxin production by A. flavus was 85 ± 1% for 21 days at 30C.

Taxon
peanutsArachis hypogaeat0
Interactor
A. flavusAspergillus flavuse1pathogen
Outcome
limiting relative humidity for aflatoxin productione1
Value
85ŷ1% relative humiditye1
Qualifier
for 21 days at 30Ce1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arachis hypogaea
Arachis hypogaea · Taxon葉子 · CC0 · iNaturalist ↗
Aspergillus flavus
Aspergillus flavus · InteractorUS gov · Public domain · Wikimedia Commons ↗

Verbatim

t0

“in sterile peanuts”

✓ located verbatim in the source · title, characters 124–142 · show in text ↑
e1

“The limiting relative humidity for aflatoxin production by A. flavus was 85ŷ1% relative humidity for 21 days at 30C”

✓ located verbatim in the source · abstract, characters 492–607 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The limiting low temperature for visible growth and aflatoxin production was 13 ± 1C for 21 days.

Taxon
peanutsArachis hypogaeat0
Outcome
limiting low temperature for visible growth and aflatoxin productione2
Value
13ŷ1Ce2
Qualifier
for 21 dayse2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arachis hypogaea
Arachis hypogaea · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

t0

“in sterile peanuts”

✓ located verbatim in the source · title, characters 124–142 · show in text ↑
e2

“The limiting low temperature for visible growth and aflatoxin production by the fungus was 13ŷ1C for 21 days”

✓ located verbatim in the source · abstract, characters 609–717 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Damaged kernels developed some aflatoxin after 21 days at 12C.

Taxon
peanutsArachis hypogaeat0
Organ / stage
Damaged kernelse3
Outcome
afllatoxine3
Qualifier
in 21 days at 12Ce3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arachis hypogaea
Arachis hypogaea · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

t0

“in sterile peanuts”

✓ located verbatim in the source · title, characters 124–142 · show in text ↑
e3

“Damaged kernels, however, developed some afllatoxin in 21 days at 12C”

✓ located verbatim in the source · abstract, characters 747–816 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The maximum temperature for aflatoxin production was 41.5 ± 1.5C for 21 days.

Taxon
peanutsArachis hypogaeat0
Outcome
maximum temperature for aflatoxin productione4
Value
41.5ŷ1.5Ce4
Qualifier
for 21 dayse4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arachis hypogaea
Arachis hypogaea · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

t0

“in sterile peanuts”

✓ located verbatim in the source · title, characters 124–142 · show in text ↑
e4

“The maximum temperature for aflatoxin production was 41.5ŷ1.5C for 21 days”

✓ located verbatim in the source · abstract, characters 818–892 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Fungal growth and sporulation at 43C were equal to those at 40C.

Taxon
peanutsArachis hypogaeat0
Treatment
43Ce5
Outcome
Fungus growth and sporulatione5
Direction
equale5
Comparator
40Ce5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arachis hypogaea
Arachis hypogaea · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

t0

“in sterile peanuts”

✓ located verbatim in the source · title, characters 124–142 · show in text ↑
e5

“Fungus growth and sporulation at 43C were equal to that at 40C, but no aflatoxin was produced”

✓ located verbatim in the source · abstract, characters 922–1015 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

No aflatoxin was produced at 43C.

Taxon
peanutsArachis hypogaeat0
Treatment
43Ce5
Outcome
no aflatoxin was producede5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arachis hypogaea
Arachis hypogaea · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

t0

“in sterile peanuts”

✓ located verbatim in the source · title, characters 124–142 · show in text ↑
e5

“Fungus growth and sporulation at 43C were equal to that at 40C, but no aflatoxin was produced”

✓ located verbatim in the source · abstract, characters 922–1015 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Moisture content of immature kernels was higher at equilibrium with the same relative humidity than in sound mature, damaged or unshelled-peanut kernels.

Taxon
peanutsArachis hypogaeat0
Organ / stage
immature kernelse6
Outcome
moisture contente6
Direction
highere6
Qualifier
at equilibrium with the same relative humiditye6
Comparator
sound mature kernels, damaged kernels, or kernels from unshelled peanutse6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arachis hypogaea
Arachis hypogaea · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

t0

“in sterile peanuts”

✓ located verbatim in the source · title, characters 124–142 · show in text ↑
e6

“Moisture content of immature kernels was higher at equilibrium with the same relative humidity than the moisture content of sound mature kernels, damaged kernels, or kernels from unshelled peanuts”

✓ located verbatim in the source · abstract, characters 1017–1213 · show in text ↑
Associationobservedoriginal resultno effectmeasured

Claim · model-written summary

No proportional quantitative correlation was found between aflatoxin synthesis and free fatty acid production in nonliving peanuts.

Taxon
peanutsArachis hypogaeat0
Material
nonliving peanutse7
Compound
free fatty acidse7
Outcome
proportional quantitative correlation between synthesis of aflatoxin and production of free fatty acidse7

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arachis hypogaea
Arachis hypogaea · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

t0

“in sterile peanuts”

✓ located verbatim in the source · title, characters 124–142 · show in text ↑
e7

“There appeared to be no proportional quantitative correlation between synthesis of aflatoxin and production of free fatty acids in nonliving peanuts”

✓ located verbatim in the source · abstract, characters 1215–1363 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Aflatoxin was not produced without a simultaneous increase in free fatty acids.

Taxon
peanutsArachis hypogaeat0
Compound
free fatty acidse1
Outcome
aflatoxine1
Qualifier
without a simultaneous increasee1

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arachis hypogaea
Arachis hypogaea · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

t0

“in sterile peanuts”

✓ located verbatim in the source · title, characters 124–142 · show in text ↑
e1

“but no aflatoxin was produced without a simultaneous increase in free fatty acids”

✓ located verbatim in the source · abstract, characters 1365–1446 · show in text ↑