Abstract
Glyphosate tolerant cell lines were selected from highly embryogenic cell suspension culture ofMedicago sativa L. Resistant cell lines showed significant reduction of embryogenic ability and during long-term culture in the presence of glyphosate gradual loss of this ability was observed. After glyphosate treatment the increased activity of 5-enolpyruvylshikimate-3-phosphate synthase in tolerant cell lines overcame the block in aromatic amino acid synthesis which was observed in control cell lines. Glyphosate caused marked increase in the content of shikimic acid in both control and tolerant cell lines but the accumulation of shikimic acid was considerably lower in tolerant calli. Significant qualitative and quantitative differences were found in the content of individual phenolic acids. The considerable decrease in the amount of cinnamic acid derivates and broader spectrum of hydroxybenzoic acids suggest in tolerant cell lines the activation of alternative pathway not regulated by phenylalanine ammonia lyase. The possible role of altered pool of phenolic acids on the embryogenic ability is discussed.
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Claims 9
Claim · model-written summary
Glyphosate-tolerant alfalfa cell lines showed a significant reduction of embryogenic ability.
- Taxon
- alfalfa
Medicago sativat0 - Material
- Resistant cell linese1
- Outcome
- embryogenic abilitye1
- Direction
- reductione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Changes of shikimate pathway in glyphosate tolerant alfalfa cell lines with reduced embryogenic ability”
e1“Resistant cell lines showed significant reduction of embryogenic ability”
Claim · model-written summary
Gradual loss of embryogenic ability occurred during long-term culture in the presence of glyphosate.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- long-term culture in the presence of glyphosatee1
- Outcome
- loss of this abilitye1
- Direction
- gradual losse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Changes of shikimate pathway in glyphosate tolerant alfalfa cell lines with reduced embryogenic ability”
e1“during long-term culture in the presence of glyphosate gradual loss of this ability was observed”
Claim · model-written summary
After glyphosate treatment, EPSPS activity increased in tolerant cell lines and overcame the aromatic amino acid synthesis block seen in control cell lines.
- Taxon
- alfalfaMedicago sativat0
- Material
- tolerant cell linese1
- Treatment
- glyphosate treatmente1
- Gene
- 5-enolpyruvylshikimate-3-phosphate synthasee1
- Outcome
- activity of 5-enolpyruvylshikimate-3-phosphate synthasee1
- Direction
- increasede1
- Comparator
- control cell linese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Changes of shikimate pathway in glyphosate tolerant alfalfa cell lines with reduced embryogenic ability”
e1“After glyphosate treatment the increased activity of 5-enolpyruvylshikimate-3-phosphate synthase in tolerant cell lines overcame the block in aromatic amino acid synthesis which was observed in control cell lines”
Claim · model-written summary
Glyphosate increased shikimic acid content in both control and tolerant cell lines.
- Taxon
- alfalfaMedicago sativat0
- Material
- control and tolerant cell linese1
- Treatment
- Glyphosatee1
- Outcome
- content of shikimic acide1
- Direction
- increasee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Changes of shikimate pathway in glyphosate tolerant alfalfa cell lines with reduced embryogenic ability”
e1“Glyphosate caused marked increase in the content of shikimic acid in both control and tolerant cell lines but the accumulation of shikimic acid was considerably lower in tolerant calli”
Claim · model-written summary
Shikimic acid accumulation after glyphosate was considerably lower in tolerant calli than in control cells.
- Taxon
- alfalfaMedicago sativat0
- Material
- tolerant callie1
- Treatment
- Glyphosatee1
- Outcome
- accumulation of shikimic acide1
- Direction
- lowere1
- Comparator
- controle1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Changes of shikimate pathway in glyphosate tolerant alfalfa cell lines with reduced embryogenic ability”
e1“Glyphosate caused marked increase in the content of shikimic acid in both control and tolerant cell lines but the accumulation of shikimic acid was considerably lower in tolerant calli”
Claim · model-written summary
Significant qualitative and quantitative differences were found in the content of individual phenolic acids.
- Taxon
- alfalfaMedicago sativat0
- Outcome
- content of individual phenolic acidse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Changes of shikimate pathway in glyphosate tolerant alfalfa cell lines with reduced embryogenic ability”
e1“Significant qualitative and quantitative differences were found in the content of individual phenolic acids”
Claim · model-written summary
Cinnamic acid derivatives decreased in tolerant cell lines.
- Taxon
- alfalfaMedicago sativat0
- Material
- tolerant cell linese1
- Outcome
- amount of cinnamic acid derivatese1
- Direction
- decreasee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Changes of shikimate pathway in glyphosate tolerant alfalfa cell lines with reduced embryogenic ability”
e1“The considerable decrease in the amount of cinnamic acid derivates and broader spectrum of hydroxybenzoic acids suggest in tolerant cell lines the activation of alternative pathway not regulated by phenylalanine ammonia lyase”
Claim · model-written summary
Tolerant cell lines had a broader spectrum of hydroxybenzoic acids.
- Taxon
- alfalfaMedicago sativat0
- Material
- tolerant cell linese1
- Outcome
- spectrum of hydroxybenzoic acidse1
- Direction
- broadere1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Changes of shikimate pathway in glyphosate tolerant alfalfa cell lines with reduced embryogenic ability”
e1“The considerable decrease in the amount of cinnamic acid derivates and broader spectrum of hydroxybenzoic acids suggest in tolerant cell lines the activation of alternative pathway not regulated by phenylalanine ammonia lyase”
Claim · model-written summary
Tolerant cell lines may activate an alternative phenolic pathway not regulated by phenylalanine ammonia lyase.
- Taxon
- alfalfaMedicago sativat0
- Material
- tolerant cell linese1
- Gene
- phenylalanine ammonia lyasee1
- Outcome
- activation of alternative pathwaye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Changes of shikimate pathway in glyphosate tolerant alfalfa cell lines with reduced embryogenic ability”
e1“The considerable decrease in the amount of cinnamic acid derivates and broader spectrum of hydroxybenzoic acids suggest in tolerant cell lines the activation of alternative pathway not regulated by phenylalanine ammonia lyase”
The same questions in other papers
glyphosate · molecular: metabolites · Medicago sativa (alfalfa)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.