Abstract
The effects of osmotic dehydration in wheat leaves ( Triticum aestivum L. cv. Longchun No. 10) on the photochemical function and protein metabolism of PSII were studied with isolated thylakoid and PSII membranes. The results indicated that PSII was rather resistant to water stress as mild water deficit in leaves did nut significantly affect its activity. However, extreme stress conditions such as 40% decrease in relative water content (RWC) or 1.8 MPa in water potential (Ψ) caused ca 50% reduction in O 2 evolution and ca 25% inhibition of DCIP (2.6‐dichlorophenol indophenol) photoreduction of PSII. In addition, it was found that the inhibited DCIP photoreduction of PSII could not be reversed by DPC (2.2‐diphenylcarbazide), a typical electron donor to PSII, suggesting that water stress did not affect electron donation to PSII. Urea‐SDS‐PAGE and western blot analysis showed that the steady slate levels of major PSII proteins, including the D1 and D2 proteins in the PSII reaction center, declined on a chlorophyll basis with increasing water stress, possibly as a result of increased degradation. In vitro translation experiments and quantitative analysis of chloroplast RNAs indicated that the potential synthesis of chloroplast proteins from their mRNAs was impaired by water stress. From the results it is concluded that the effects of water stress on PSII protein metabolism, especially on the reaction center proteins, may account for the damage to PSII photochemistry.
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Claims 8
Claim · model-written summary
Mild leaf water deficit did not significantly affect PSII activity in wheat.
- Taxon
- wheat
Triticum aestivumt0 - Treatment
- mild water deficite0
- Outcome
- activitye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mild water deficit in leaves did nut significantly affect its activity”
t0“wheat leaves”
Claim · model-written summary
Extreme water stress reduced PSII oxygen evolution by about 50%.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- extreme stress conditionse0
- Outcome
- O 2 evolutione0
- Direction
- reductione0
- Value
- ca 50%e0
- Qualifier
- 40% decrease in relative water content (RWC) or 1.8 MPa in water potential (Ψ)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“extreme stress conditions such as 40% decrease in relative water content (RWC) or 1.8 MPa in water potential (Ψ) caused ca 50% reduction in O 2 evolution”
t0“wheat leaves”
Claim · model-written summary
Extreme water stress inhibited PSII DCIP photoreduction by about 25%.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- extreme stress conditionse0
- Outcome
- DCIP (2.6‐dichlorophenol indophenol) photoreduction of PSIIe0
- Direction
- inhibitione0
- Value
- ca 25%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“extreme stress conditions such as 40% decrease in relative water content (RWC) or 1.8 MPa in water potential (Ψ) caused ca 50% reduction in O 2 evolution and ca 25% inhibition of DCIP (2.6‐dichlorophenol indophenol) photoreduction of PSII”
t0“wheat leaves”
Claim · model-written summary
DPC did not reverse inhibited PSII DCIP photoreduction.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- DPC (2.2‐diphenylcarbazide)e0
- Outcome
- inhibited DCIP photoreduction of PSIIe0
- Direction
- could not be reversede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the inhibited DCIP photoreduction of PSII could not be reversed by DPC (2.2‐diphenylcarbazide)”
t0“wheat leaves”
Claim · model-written summary
Water stress was inferred not to affect electron donation to PSII.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- water stresse0
- Outcome
- electron donation to PSIIe0
- Direction
- did not affecte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggesting that water stress did not affect electron donation to PSII”
t0“wheat leaves”
Claim · model-written summary
Major PSII proteins, including D1 and D2, declined on a chlorophyll basis as water stress increased.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- water stresse0
- Gene
- D1 and D2 proteinse0
- Outcome
- steady slate levels of major PSII proteinse0
- Direction
- declinede0
- Qualifier
- on a chlorophyll basise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“steady slate levels of major PSII proteins, including the D1 and D2 proteins in the PSII reaction center, declined on a chlorophyll basis with increasing water stress”
t0“wheat leaves”
Claim · model-written summary
Water stress impaired the potential synthesis of chloroplast proteins from their mRNAs.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- water stresse0
- Outcome
- potential synthesis of chloroplast proteins from their mRNAse0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the potential synthesis of chloroplast proteins from their mRNAs was impaired by water stress”
t0“wheat leaves”
Claim · model-written summary
Water-stress effects on PSII protein metabolism may explain damage to PSII photochemistry.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- water stresse0
- Outcome
- damage to PSII photochemistrye0
- Qualifier
- effects of water stress on PSII protein metabolisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the effects of water stress on PSII protein metabolism, especially on the reaction center proteins, may account for the damage to PSII photochemistry”
t0“wheat leaves”
The same questions in other papers
drought · molecular: metabolites · Triticum aestivum (wheat)Positive and negative results
68 papers in the same direction as this paper · 56 in the opposite direction
- no effect
- positive
- positive
121 papers more in this group
See all 125 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.