Abstract
Despite the recent discovery that trehalose synthesis is widespread in higher plants very little is known about its physiological significance. Here we report on an Arabidopsis mutant (tps1), disrupted in a gene encoding the first enzyme of trehalose biosynthesis (trehalose-6-phosphate synthase). The tps1 mutant is a recessive embryo lethal. Embryo morphogenesis is normal but development is retarded and stalls early in the phase of cell expansion and storage reserve accumulation. TPS1 is transiently up-regulated at this same developmental stage and is required for the full expression of seed maturation marker genes (2S2 and OLEOSN2). Sucrose levels also increase rapidly in seeds during the onset of cell expansion. In Saccharomyces cerevisiae trehalose-6-phosphate (T-6-P) is required to regulate sugar influx into glycolysis via the inhibition of hexokinase and a deficiency in TPS1 prevents growth on sugars (Thevelein and Hohmann, 1995). The growth of Arabidopsis tps1-1 embryos can be partially rescued in vitro by reducing the sucrose level. However, T-6-P is not an inhibitor of AtHXK1 or AtHXK2. Nor does reducing hexokinase activity rescue tps1-1 embryo growth. Our data establish for the first time that an enzyme of trehalose metabolism is essential in plants and is implicated in the regulation of sugar metabolism/embryo development via a different mechanism to that reported in S. cerevisiae.
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Claims 8
Claim · model-written summary
The Arabidopsis tps1 mutant is recessive embryo lethal.
- Taxon
- Arabidopsis
Arabidopsist0 - Material
- tps1 mutante0
- Outcome
- recessive embryo lethale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The tps1 mutant is a recessive embryo lethal”
t0“Arabidopsis”
Claim · model-written summary
tps1 embryo morphogenesis is normal, while development is delayed and stalls at early cell expansion and storage reserve accumulation.
- Taxon
- ArabidopsisArabidopsist0
- Material
- tps1e1
- Outcome
- development is retarded and stalls early in the phase of cell expansion and storage reserve accumulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Embryo morphogenesis is normal but development is retarded and stalls early in the phase of cell expansion and storage reserve accumulation”
e1“The tps1 mutant is a recessive embryo lethal”
t0“Arabidopsis”
Claim · model-written summary
TPS1 is transiently up-regulated during early cell expansion and storage reserve accumulation.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- phase of cell expansion and storage reserve accumulatione1
- Gene
- TPS1e0
- Outcome
- TPS1 is transiently up-regulatede0
- Direction
- up-regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TPS1 is transiently up-regulated at this same developmental stage”
e1“phase of cell expansion and storage reserve accumulation”
t0“Arabidopsis”
Claim · model-written summary
TPS1 is needed for full expression of seed maturation marker genes 2S2 and OLEOSN2.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- TPS1e1
- Outcome
- full expression of seed maturation marker genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“is required for the full expression of seed maturation marker genes (2S2 and OLEOSN2)”
e1“TPS1 is transiently up-regulated”
t0“Arabidopsis”
Claim · model-written summary
Seed sucrose levels rise rapidly at the onset of cell expansion.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- seedse0
- Outcome
- Sucrose levelse0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sucrose levels also increase rapidly in seeds during the onset of cell expansion”
t0“Arabidopsis”
Claim · model-written summary
Reducing sucrose partially rescues Arabidopsis tps1-1 embryo growth in vitro.
- Taxon
- ArabidopsisArabidopsise0
- Material
- tps1-1e0
- Treatment
- reducing the sucrose levele0
- Outcome
- growth of Arabidopsis tps1-1 embryose0
- Direction
- partially rescuede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The growth of Arabidopsis tps1-1 embryos can be partially rescued in vitro by reducing the sucrose level”
Claim · model-written summary
T-6-P does not inhibit AtHXK1 or AtHXK2.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- AtHXK1 or AtHXK2e0
- Outcome
- not an inhibitor of AtHXK1 or AtHXK2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“T-6-P is not an inhibitor of AtHXK1 or AtHXK2”
t0“Arabidopsis”
Claim · model-written summary
Reducing hexokinase activity does not rescue tps1-1 embryo growth.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- reducing hexokinase activitye0
- Gene
- hexokinasee0
- Outcome
- tps1-1 embryo growthe0
- Direction
- Nor doese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nor does reducing hexokinase activity rescue tps1-1 embryo growth”
t0“Arabidopsis”
The same questions in other papers
sucrose · growth · ArabidopsisPositive and negative results
8 papers in the same direction as this paper · 8 in the opposite direction
- negative
- positive
- negative
13 papers more in this group
See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.