Abstract
When root tips of fully aerobic, intact maize (Zea mays L.) seedlings are made anaerobic, viability normally is only 24 hours or less at 25 degrees C. We find that viability can be extended to at least 96 hours if seedlings are given a hypoxic pretreatment for 18 hours by sparging the solution with 4% O(2) in nitrogen (v/v) before anoxia. Fully aerobic root tips (sparged with 40% O(2)) had very low alcohol dehydrogenase (ADH) activity (per gram root fresh weight), and the level remained low under anoxia. In hypoxically pretreated roots, however, high levels of ADH activity were induced, and activity rose further during the initial 24 hours of anoxia, and then remained high at about 20 times that of controls in 40% O(2). ADH activity in roots in solution sparged with air (21% O(2)) was about three times that in 40% O(2). Improved viability of hypoxically pretreated root tips was associated with maintenance of a high energy metabolism (ATP concentration, total adenylates, and adenylate energy charge). Roots that were not pretreated lost 94% of the total adenylates and ATP at 24 hours of anoxia. The relation between induced ADH activity, energy metabolism, and improved anoxia-tolerance in acclimated maize root tips is discussed.
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Claims 6
Claim · model-written summary
Hypoxic pretreatment extended maize root-tip viability under anoxia to at least 96 hours, whereas roots not pretreated lost viability within about 24 hours
- Organ / stage
- root tipse0
- Treatment
- hypoxic pretreatment for 18 hourse1
- Outcome
- viabilitye1
- Direction
- extendede1
- Value
- at least 96 hourse1
- Qualifier
- viability normally is only 24 hours or lesse0
- Comparator
- Roots that were not pretreatede3
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When root tips of fully aerobic, intact maize (Zea mays L.) seedlings are made anaerobic, viability normally is only 24 hours or less at 25 degrees C”
e1“viability can be extended to at least 96 hours if seedlings are given a hypoxic pretreatment for 18 hours by sparging the solution with 4% O(2) in nitrogen (v/v) before anoxia”
e3“Roots that were not pretreated lost 94% of the total adenylates and ATP at 24 hours of anoxia”
Claim · model-written summary
Hypoxic pretreatment induced ADH activity in maize root tips, about 20 times that of aerobic controls at 40% O2 during anoxia
- Organ / stage
- root tipse6
- Treatment
- hypoxic pretreatment for 18 hourse1
- Gene
- alcohol dehydrogenase (ADH)e6
- Outcome
- ADH activitye2
- Direction
- inducede2
- Value
- about 20 timese2
- Comparator
- controls in 40% O(2)e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When root tips of fully aerobic, intact maize (Zea mays L.) seedlings are made anaerobic, viability normally is only 24 hours or less at 25 degrees C”
e1“viability can be extended to at least 96 hours if seedlings are given a hypoxic pretreatment for 18 hours by sparging the solution with 4% O(2) in nitrogen (v/v) before anoxia”
e2“In hypoxically pretreated roots, however, high levels of ADH activity were induced, and activity rose further during the initial 24 hours of anoxia, and then remained high at about 20 times that of controls in 40% O(2)”
e6“Fully aerobic root tips (sparged with 40% O(2)) had very low alcohol dehydrogenase (ADH) activity (per gram root fresh weight), and the level remained low under anoxia”
Claim · model-written summary
ADH activity in maize roots sparged with air was about three times that at 40% O2
- Treatment
- sparged with air (21% O(2))e5
- Outcome
- ADH activitye5
- Direction
- higherinferrede5
- Value
- about three timese5
- Comparator
- 40% O(2)e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“ADH activity in roots in solution sparged with air (21% O(2)) was about three times that in 40% O(2)”
e0“When root tips of fully aerobic, intact maize (Zea mays L.) seedlings are made anaerobic, viability normally is only 24 hours or less at 25 degrees C”
Claim · model-written summary
Fully aerobic maize root tips had very low ADH activity, and the level remained low under anoxia
- Organ / stage
- Fully aerobic root tipse6
- Outcome
- alcohol dehydrogenase (ADH) activitye6
- Direction
- very lowe6
- Qualifier
- remained low under anoxiae6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“Fully aerobic root tips (sparged with 40% O(2)) had very low alcohol dehydrogenase (ADH) activity (per gram root fresh weight), and the level remained low under anoxia”
e0“When root tips of fully aerobic, intact maize (Zea mays L.) seedlings are made anaerobic, viability normally is only 24 hours or less at 25 degrees C”
Claim · model-written summary
Improved viability of hypoxically pretreated maize root tips was associated with maintained high energy metabolism
- Treatment
- hypoxically pretreated root tipse4
- Outcome
- viabilitye4
- Direction
- Improvede4
- Qualifier
- maintenance of a high energy metabolisme4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Improved viability of hypoxically pretreated root tips was associated with maintenance of a high energy metabolism”
e0“When root tips of fully aerobic, intact maize (Zea mays L.) seedlings are made anaerobic, viability normally is only 24 hours or less at 25 degrees C”
Claim · model-written summary
Maize root tips that were not pretreated lost 94% of total adenylates and ATP at 24 hours of anoxia
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Roots that were not pretreated lost 94% of the total adenylates and ATP at 24 hours of anoxia”
e0“When root tips of fully aerobic, intact maize (Zea mays L.) seedlings are made anaerobic, viability normally is only 24 hours or less at 25 degrees C”
The same questions in other papers
flooding · growth · Zea mays (maize)Results differ
11 papers in the same direction as this paper · 56 in the opposite direction
- negative
- negative
- negative
64 papers more in this group
See all 68 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.