Abstract
Radioactive sucrose, l-leucine, l-glutamate, and gamma-aminobutyrate were applied exogenously to abraded areas of soybean leaves. The three amino acids were translocated with similar velocities and mass transfer rates on a molar basis, although they were metabolized differently in the sink tissue. The concentration dependence of leucine translocation showed a triphasic saturation response, while sucrose translocation showed a biphasic saturation response to increasing concentration. Apparent K(m) and V(max) for leucine and sucrose loading in the phloem differed. Both leucine and sucrose translocation were inhibited by uncouplers, high K(+), and p-chloromercuribenzenesulfonic acid. Treatment with 0.8 m sorbitol had little effect on sucrose translocation but stimulated leucine translocation, indicating an apoplastic route of loading for leucine. No effect on mass transfer rates was observed when sucrose and amino acids were applied exogenously together. These data provide evidence that phloem loading of amino acids and sucrose is mediated by different and separate carriers, both being dependent on an energy-requiring mechanism.
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Claims 10
Claim · model-written summary
Three amino acids translocated at similar velocity and molar mass transfer rates.
- Taxon
- Soybean
Glycine maxt0 - Outcome
- similar velocities and mass transfer ratese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The three amino acids were translocated with similar velocities and mass transfer rates on a molar basis”
t0“Soybean”
Claim · model-written summary
The amino acids were metabolized differently in sink tissue.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- metabolized differentlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“although they were metabolized differently in the sink tissue”
t0“Soybean”
Claim · model-written summary
Leucine translocation showed triphasic concentration saturation.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- triphasic saturation responsee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The concentration dependence of leucine translocation showed a triphasic saturation response”
t0“Soybean”
Claim · model-written summary
Sucrose translocation showed biphasic concentration saturation.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- biphasic saturation responsee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sucrose translocation showed a biphasic saturation response to increasing concentration”
t0“Soybean”
Claim · model-written summary
Leucine and sucrose loading had different apparent kinetic parameters.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- K(m) and V(max)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Apparent K(m) and V(max) for leucine and sucrose loading in the phloem differed”
t0“Soybean”
Claim · model-written summary
Uncouplers, high potassium, and p-chloromercuribenzenesulfonic acid inhibited both translocations.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- uncouplers, high K(+), and p-chloromercuribenzenesulfonic acide0
- Outcome
- leucine and sucrose translocatione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both leucine and sucrose translocation were inhibited by uncouplers, high K(+), and p-chloromercuribenzenesulfonic acid”
t0“Soybean”
Claim · model-written summary
Sorbitol had little effect on sucrose translocation.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- 0.8 m sorbitole0
- Outcome
- sucrose translocatione0
- Direction
- little effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment with 0.8 m sorbitol had little effect on sucrose translocation”
t0“Soybean”
Claim · model-written summary
Sorbitol stimulated leucine translocation.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- 0.8 m sorbitole0
- Outcome
- leucine translocatione0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment with 0.8 m sorbitol had little effect on sucrose translocation but stimulated leucine translocation”
t0“Soybean”
Claim · model-written summary
Joint application of sucrose and amino acids did not alter mass transfer rates.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- sucrose and amino acids were applied exogenously togethere0
- Outcome
- mass transfer ratese0
- Direction
- No effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No effect on mass transfer rates was observed when sucrose and amino acids were applied exogenously together”
t0“Soybean”
Claim · model-written summary
The authors infer different energy dependent carriers for sucrose and amino acids.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- phloem loading of amino acids and sucrosee0
- Qualifier
- different and separate carrierse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“phloem loading of amino acids and sucrose is mediated by different and separate carriers, both being dependent on an energy-requiring mechanism”
t0“Soybean”
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See all 36 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.