Abstract
Hexokinase (HXK, EC 2.7.1.1) plays an important role in the metabolism and glucose signalling. To examine the characteristics of HXK gene family in rice, the subcellular localizations of ten hexokinases (OsHXK1 - OsHXK10) were determined using OsHXK::GFP fusion proteins in tobacco mesophyll protoplasts. As was previously demonstrated, OsHXK4 was detected in the chloroplast stroma, OsHXK5 and OsHXK6 in the mitochondria, and OsHXK7 and OsHXK10 in the cytoplasm. In the present study, OsHXKs were clearly divided into three types (A, B, C) based on their N-terminal sequences. The new type-C HXKs in plants, OsHXK1, OsHXK7 and OsHXK8, which lack the plastidic transit peptide and the membrane anchor domain, were detected not only in the cytoplasm but also in the nucleus. The type-B HXKs, OsHXK2, OsHXK3, OsHXK9 and OsHXK10, which contained a membrane anchor domain, were distinctly localized in the mitochondria. These results suggest that OsHXKs localized in different cell compartments may be involved in the glucose signalling-related gene expression during growth and development of rice.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
OsHXKs were divided into three types by N-terminal sequence.
- Taxon
- rice
Oryza sativae1 - Gene
- OsHXKse0
- Outcome
- three types (A, B, C)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsHXKs were clearly divided into three types (A, B, C) based on their N-terminal sequences.”
e1“HXK gene family in rice”
Claim · model-written summary
OsHXK1 localized in cytoplasm and nucleus.
- Taxon
- riceOryza sativae1
- Gene
- OsHXK1e0
- Outcome
- detected not only in the cytoplasm but also in the nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The new type-C HXKs in plants, OsHXK1, OsHXK7 and OsHXK8, which lack the plastidic transit peptide and the membrane anchor domain, were detected not only in the cytoplasm but also in the nucleus.”
e1“HXK gene family in rice”
Claim · model-written summary
OsHXK7 localized in cytoplasm and nucleus.
- Taxon
- riceOryza sativae1
- Gene
- OsHXK7e0
- Outcome
- detected not only in the cytoplasm but also in the nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The new type-C HXKs in plants, OsHXK1, OsHXK7 and OsHXK8, which lack the plastidic transit peptide and the membrane anchor domain, were detected not only in the cytoplasm but also in the nucleus.”
e1“HXK gene family in rice”
Claim · model-written summary
OsHXK8 localized in cytoplasm and nucleus.
- Taxon
- riceOryza sativae1
- Gene
- OsHXK8e0
- Outcome
- detected not only in the cytoplasm but also in the nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The new type-C HXKs in plants, OsHXK1, OsHXK7 and OsHXK8, which lack the plastidic transit peptide and the membrane anchor domain, were detected not only in the cytoplasm but also in the nucleus.”
e1“HXK gene family in rice”
Claim · model-written summary
OsHXK2 localized in mitochondria.
- Taxon
- riceOryza sativae1
- Gene
- OsHXK2e0
- Outcome
- localized in the mitochondriae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The type-B HXKs, OsHXK2, OsHXK3, OsHXK9 and OsHXK10, which contained a membrane anchor domain, were distinctly localized in the mitochondria.”
e1“HXK gene family in rice”
Claim · model-written summary
OsHXK3 localized in mitochondria.
- Taxon
- riceOryza sativae1
- Gene
- OsHXK3e0
- Outcome
- localized in the mitochondriae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The type-B HXKs, OsHXK2, OsHXK3, OsHXK9 and OsHXK10, which contained a membrane anchor domain, were distinctly localized in the mitochondria.”
e1“HXK gene family in rice”
Claim · model-written summary
OsHXK9 localized in mitochondria.
- Taxon
- riceOryza sativae1
- Gene
- OsHXK9e0
- Outcome
- localized in the mitochondriae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The type-B HXKs, OsHXK2, OsHXK3, OsHXK9 and OsHXK10, which contained a membrane anchor domain, were distinctly localized in the mitochondria.”
e1“HXK gene family in rice”
Claim · model-written summary
OsHXK10 localized in mitochondria.
- Taxon
- riceOryza sativae1
- Gene
- OsHXK10e0
- Outcome
- localized in the mitochondriae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The type-B HXKs, OsHXK2, OsHXK3, OsHXK9 and OsHXK10, which contained a membrane anchor domain, were distinctly localized in the mitochondria.”
e1“HXK gene family in rice”
Claim · model-written summary
Compartment-specific OsHXKs may participate in glucose-signaling gene expression during rice development.
- Taxon
- riceOryza sativae0
- Gene
- OsHXKse0
- Outcome
- glucose signalling-related gene expressione0
- Qualifier
- may be involvede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsHXKs localized in different cell compartments may be involved in the glucose signalling-related gene expression during growth and development of rice.”