Abstract
Cycloheximide inhibits the incorporation of acetate-2- 14 C into protein and into asparagine in corn root tips. It also causes an accumulation of glutamine and, over a concentration range of 0.4 to 5.0 μg/ml, a transient accumulation of the neutral and basic amino acids. In mature sections, cycloheximide inhibits protein synthesis but causes an increase in the incorporation of radioactivity into both glutamine and asparagine. Azaserine, a glutamine analogue, also inhibits the formation of asparagine in root-tip sections but has only a minor effect on protein synthesis. In mature root sections, there is an accumulation of glutamine but no effect on asparagine formation when azaserine is used. Glutamine additions to root tips or mature root sections affect neither asparagine formation nor protein synthesis. We conclude that cycloheximide is behaving as a glutamine analogue in its effect on asparagine biosynthesis, and that its effect as a glutamine analogue is lost as cells mature.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 12
Claim · model-written summary
Cycloheximide inhibited labelled acetate incorporation into protein in corn root tips.
- Taxon
- corn
Zea mays subsp. mayse0 - Organ / stage
- root tipse0
- Treatment
- Cycloheximidee0
- Outcome
- incorporation of acetate-2- 14 C into proteine0
- Direction
- inhibitse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cycloheximide inhibits the incorporation of acetate-2- 14 C into protein and into asparagine in corn root tips”
Claim · model-written summary
Cycloheximide inhibited labelled acetate incorporation into asparagine in corn root tips.
- Taxon
- cornZea mays subsp. mayse0
- Organ / stage
- root tipse0
- Treatment
- Cycloheximidee0
- Outcome
- asparaginee0
- Direction
- inhibitse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cycloheximide inhibits the incorporation of acetate-2- 14 C into protein and into asparagine in corn root tips”
Claim · model-written summary
Cycloheximide caused glutamine accumulation in corn root tips.
- Organ / stage
- rootst0
- Treatment
- cycloheximidet0
- Outcome
- accumulation of glutaminee0
- Direction
- accumulatione0
Zea maysNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It also causes an accumulation of glutamine and, over a concentration range of 0.4 to 5.0 μg/ml, a transient accumulation of the neutral and basic amino acids”
t0“cycloheximide on amide formation in maize roots”
Claim · model-written summary
Cycloheximide transiently accumulated neutral and basic amino acids in corn root tips at 0.4 to 5.0 μg/ml.
- Organ / stage
- rootst0
- Treatment
- cycloheximidet0
- Outcome
- transient accumulation of the neutral and basic amino acidse0
- Direction
- accumulatione0
- Value
- 0.4 to 5.0 μg/mle0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It also causes an accumulation of glutamine and, over a concentration range of 0.4 to 5.0 μg/ml, a transient accumulation of the neutral and basic amino acids”
t0“cycloheximide on amide formation in maize roots”
Claim · model-written summary
Cycloheximide inhibited protein synthesis in mature root sections.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In mature sections, cycloheximide inhibits protein synthesis but causes an increase in the incorporation of radioactivity into both glutamine and asparagine”
t0“maize roots”
Claim · model-written summary
Cycloheximide increased radioactive incorporation into glutamine and asparagine in mature root sections.
- Organ / stage
- mature sectionse0
- Treatment
- cycloheximidee0
- Outcome
- incorporation of radioactivity into both glutamine and asparaginee0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In mature sections, cycloheximide inhibits protein synthesis but causes an increase in the incorporation of radioactivity into both glutamine and asparagine”
t0“maize roots”
Claim · model-written summary
Azaserine inhibited asparagine formation in root-tip sections.
- Organ / stage
- root-tip sectionse0
- Treatment
- Azaserinee0
- Outcome
- formation of asparaginee0
- Direction
- inhibitse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Azaserine, a glutamine analogue, also inhibits the formation of asparagine in root-tip sections but has only a minor effect on protein synthesis”
t0“maize roots”
Claim · model-written summary
Azaserine had only a minor effect on protein synthesis in root-tip sections.
- Organ / stage
- root-tip sectionse0
- Treatment
- Azaserinee0
- Outcome
- protein synthesise0
- Qualifier
- only a minor effecte0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Azaserine, a glutamine analogue, also inhibits the formation of asparagine in root-tip sections but has only a minor effect on protein synthesis”
t0“maize roots”
Claim · model-written summary
Azaserine caused glutamine accumulation in mature root sections.
- Organ / stage
- mature root sectionse0
- Treatment
- azaserinee0
- Outcome
- accumulation of glutaminee0
- Direction
- accumulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In mature root sections, there is an accumulation of glutamine but no effect on asparagine formation when azaserine is used”
t0“maize roots”
Claim · model-written summary
Azaserine did not affect asparagine formation in mature root sections.
- Organ / stage
- mature root sectionse0
- Treatment
- azaserinee0
- Outcome
- asparagine formatione0
- Direction
- no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In mature root sections, there is an accumulation of glutamine but no effect on asparagine formation when azaserine is used”
t0“maize roots”
Claim · model-written summary
Glutamine additions did not affect asparagine formation or protein synthesis in root tips or mature sections.
- Organ / stage
- root tips or mature root sectionse0
- Treatment
- Glutamine additionse0
- Outcome
- asparagine formation nor protein synthesise0
- Direction
- affect neithere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Glutamine additions to root tips or mature root sections affect neither asparagine formation nor protein synthesis”
t0“maize roots”
Claim · model-written summary
The authors infer that cycloheximide acts as a glutamine analogue for asparagine biosynthesis, with this effect lost as cells mature.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We conclude that cycloheximide is behaving as a glutamine analogue in its effect on asparagine biosynthesis, and that its effect as a glutamine analogue is lost as cells mature”
t0“maize roots”
The same questions in other papers
glutamine · quality: protein · Zea mays (maize)Positive and negative results
1 in the opposite direction
- positive
cycloheximide · molecular: metabolites · Zea mays (maize)Results agree
1 paper in the same direction as this paper
- negative
cycloheximide · quality: protein · Zea mays (maize)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.