Paper · 2000 · Food Additives & Contaminants

Deoxynivalenol and ochratoxin A in German wheat and changes of level in relation to storage parameters

Barbara Birzele; Alexander Prange; Johannes KrÄmer

7
claims
14
verbatim quotes
126
citations
3
research areas
—
Not open access

Abstract

The occurrence of the mycotoxins deoxynivalenol (DON) and ochratoxin A (OTA) in the winter wheat of 1997 and 1998 grown under organic farming conditions was investigated using ELISAs (R-Biopharm) for quantification. The influence of delayed drying of the grain after harvest on the development of DON and OTA was determined in storage trials (moisture: 17% and 20%; temperature: 20 degrees C; duration: four and six weeks). The Tox5 PCR assay was used both to detect Fusarium species with the potential to produce trichothecenes and as a measure of their relative DNA content during the storage trials. The intensity of the PCR signals was correlated with the DON concentration. Fusarium species were identified microscopically by standard methods. All the freshly harvested grain samples were contaminated with DON and showed further increases in the DON concentration during storage. OTA contamination was found in 14.3% of the 1997 samples and in 24.1% of the 1998 samples. OTA increased during storage trials of the 1997 samples but not in the 1998 samples.

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Claims 7

Observationobservedoriginal resultmeasured

Claim · model-written summary

All freshly harvested wheat grain samples contained DON.

Organ / stage
freshly harvested graine0
Outcome
contaminated with DONe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“All the freshly harvested grain samples were contaminated with DON and showed further increases in the DON concentration during storage.”

✓ located verbatim in the source · abstract, characters 749–885 · show in text ↑
t0

“German wheat”

✓ located verbatim in the source · title, characters 35–47 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

DON concentration increased during wheat grain storage.

Organ / stage
graine0
Treatment
during storagee0
Outcome
DON concentratione0
Direction
increasese0
Comparator
freshly harvested grain samplese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“All the freshly harvested grain samples were contaminated with DON and showed further increases in the DON concentration during storage.”

✓ located verbatim in the source · abstract, characters 749–885 · show in text ↑
t0

“German wheat”

✓ located verbatim in the source · title, characters 35–47 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

OTA contamination occurred in 14.3% of 1997 wheat samples.

Outcome
OTA contaminatione0
Value
14.3%e0
Qualifier
1997 samplese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“OTA contamination was found in 14.3% of the 1997 samples and in 24.1% of the 1998 samples.”

✓ located verbatim in the source · abstract, characters 886–976 · show in text ↑
t0

“German wheat”

✓ located verbatim in the source · title, characters 35–47 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

OTA contamination occurred in 24.1% of 1998 wheat samples.

Outcome
OTA contaminatione0
Value
24.1%e0
Qualifier
1998 samplese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“OTA contamination was found in 14.3% of the 1997 samples and in 24.1% of the 1998 samples.”

✓ located verbatim in the source · abstract, characters 886–976 · show in text ↑
t0

“German wheat”

✓ located verbatim in the source · title, characters 35–47 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

OTA increased during storage of the 1997 wheat samples.

Treatment
storage trialse0
Outcome
OTAe0
Direction
increasede0
Qualifier
1997 samplese0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“OTA increased during storage trials of the 1997 samples but not in the 1998 samples.”

✓ located verbatim in the source · abstract, characters 977–1061 · show in text ↑
t0

“German wheat”

✓ located verbatim in the source · title, characters 35–47 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

OTA did not increase during storage of the 1998 wheat samples.

Treatment
storage trialse0
Outcome
OTAe0
Direction
note0
Qualifier
1998 samplese0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“OTA increased during storage trials of the 1997 samples but not in the 1998 samples.”

✓ located verbatim in the source · abstract, characters 977–1061 · show in text ↑
t0

“German wheat”

✓ located verbatim in the source · title, characters 35–47 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

PCR signal intensity correlated with DON concentration.

Outcome
DON concentratione0
Qualifier
intensity of the PCR signalse0

Not reportedSettingDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The intensity of the PCR signals was correlated with the DON concentration.”

✓ located verbatim in the source · abstract, characters 603–678 · show in text ↑
t0

“German wheat”

✓ located verbatim in the source · title, characters 35–47 · show in text ↑

storage · disease · Triticum aestivum (wheat)Positive and negative results

13 papers: 2 positive, 11 negative or no effect

10 papers in the same direction as this paper · 2 in the opposite direction

  1. positive
  2. positive
  3. no effect

9 papers more in this group

See all 13 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.