Abstract
Tissue culture and histological studies were done to ascertain the effects of benzyladenine (BA)-preconditioning on direct shoot regeneration from cotyledonary node explants of mungbean. The highest direct shoot regeneration (93.3%), double-sided shoot initiation (65.0%) and average number of shoots per explant (2.7 shoots) were obtained when Pag-asa 7 explants are excised from seedlings germinated in basal medium (BM) consisting of MS salts and B5 vitamins with 1.0mgl-1 BA, and subsequently cultured in fresh medium of the same composition. Histological inspection of sections from germinating seeds in BM and BM+1.0mgl-1 BA sampled at 1 to 4 days from germination revealed bigger and developmentally more advanced axillary shoots in BA-preconditioned seedlings, a built-in advantage which could attest to the improved regenerability in BA-treated seedling explants over the control (BM only). During shoot initiation from explants, two treatments namely doubling of the concentration BA to 2.0mgl-1 and the addition of a non-ionic surfactant, Pluronic F-68 did not further increase the responses, but gave results comparable with those obtained using BM+1.0mgl-1 BA alone. Conversely, a BA-thidiazuron (TDZ) combination (each at 1.0mgl-1) when added to BM significantly reduced shoot induction to only 48.3% possibly due to increased callusing at the nodes in 55% of the explants. The importance and applicability of the regeneration system in tissue culture and/or genetic manipulations of mungbean and other Asiatic Vigna species is discussed.
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Claims 8
Claim · model-written summary
BA preconditioning produced 93.3% direct shoot regeneration in Pag-asa 7 explants.
- Taxon
- mungbean
.jpg?width=60)
Vigna radiatat0 - Material
- Pag-asa 7e0
- Treatment
- 1.0mgl-1 BAe0
- Outcome
- direct shoot regeneratione0
- Direction
- higheste0
- Value
- 93.3%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“The highest direct shoot regeneration (93.3%), double-sided shoot initiation (65.0%) and average number of shoots per explant (2.7 shoots) were obtained when Pag-asa 7 explants are excised from seedlings germinated in basal medium (BM) consisting of MS salts and B5 vitamins with 1.0mgl-1 BA, and subsequently cultured in fresh medium of the same composition”
t0“mungbean”
Claim · model-written summary
BA preconditioning produced 65.0% double-sided shoot initiation.
- Taxon
- mungbeanVigna radiatat0
- Material
- Pag-asa 7e0
- Treatment
- 1.0mgl-1 BAe0
- Outcome
- double-sided shoot initiatione0
- Direction
- higheste0
- Value
- 65.0%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest direct shoot regeneration (93.3%), double-sided shoot initiation (65.0%) and average number of shoots per explant (2.7 shoots) were obtained when Pag-asa 7 explants are excised from seedlings germinated in basal medium (BM) consisting of MS salts and B5 vitamins with 1.0mgl-1 BA, and subsequently cultured in fresh medium of the same composition”
t0“mungbean”
Claim · model-written summary
BA preconditioning yielded an average of 2.7 shoots per explant.
- Taxon
- mungbeanVigna radiatat0
- Material
- Pag-asa 7e0
- Treatment
- 1.0mgl-1 BAe0
- Outcome
- average number of shoots per explante0
- Direction
- higheste0
- Value
- 2.7 shootse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest direct shoot regeneration (93.3%), double-sided shoot initiation (65.0%) and average number of shoots per explant (2.7 shoots) were obtained when Pag-asa 7 explants are excised from seedlings germinated in basal medium (BM) consisting of MS salts and B5 vitamins with 1.0mgl-1 BA, and subsequently cultured in fresh medium of the same composition”
t0“mungbean”
Claim · model-written summary
BA-preconditioned seedlings had larger and more advanced axillary shoots than basal-medium controls.
- Taxon
- mungbeanVigna radiatat0
- Treatment
- BA-preconditionede0
- Outcome
- axillary shootse0
- Direction
- bigger and developmentally more advancede0
- Comparator
- control (BM only)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“bigger and developmentally more advanced axillary shoots in BA-preconditioned seedlings, a built-in advantage which could attest to the improved regenerability in BA-treated seedling explants over the control (BM only)”
t0“mungbean”
Claim · model-written summary
Doubling BA to 2.0mgl-1 did not further increase shoot initiation responses.
- Taxon
- mungbeanVigna radiatat0
- Treatment
- doubling of the concentration BA to 2.0mgl-1e0
- Outcome
- responsese0
- Direction
- did not further increasee0
- Comparator
- BM+1.0mgl-1 BA alonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“doubling of the concentration BA to 2.0mgl-1 and the addition of a non-ionic surfactant, Pluronic F-68 did not further increase the responses, but gave results comparable with those obtained using BM+1.0mgl-1 BA alone”
t0“mungbean”
Claim · model-written summary
Pluronic F-68 addition did not further increase shoot initiation responses.
- Taxon
- mungbeanVigna radiatat0
- Treatment
- Pluronic F-68e0
- Outcome
- responsese0
- Direction
- did not further increasee0
- Comparator
- BM+1.0mgl-1 BA alonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“doubling of the concentration BA to 2.0mgl-1 and the addition of a non-ionic surfactant, Pluronic F-68 did not further increase the responses, but gave results comparable with those obtained using BM+1.0mgl-1 BA alone”
t0“mungbean”
Claim · model-written summary
BA and TDZ together significantly reduced shoot induction to 48.3%.
- Taxon
- mungbeanVigna radiatat0
- Treatment
- BA-thidiazuron (TDZ) combination (each at 1.0mgl-1)e0
- Outcome
- shoot inductione0
- Direction
- reducede0
- Value
- 48.3%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a BA-thidiazuron (TDZ) combination (each at 1.0mgl-1) when added to BM significantly reduced shoot induction to only 48.3%”
t0“mungbean”
Claim · model-written summary
BA and TDZ together caused callusing in 55% of explants, possibly explaining reduced shoot induction.
- Taxon
- mungbeanVigna radiatat0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“possibly due to increased callusing at the nodes in 55% of the explants”
e1“a BA-thidiazuron (TDZ) combination (each at 1.0mgl-1)”
t0“mungbean”
The same questions in other papers
N-benzyladenine · growth · Vigna radiata (mung bean)Results agree
9 papers in the same direction as this paper
- positive
- positive
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See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.